Biomechanical and structural characteristics of canine femoral and tibial cartilage.

To analyze the interrelationships between the structure, composition, and mechanical properties of articular cartilage, canine knee (n = 10) femoral and tibial cartilages were used as experimental tissues. The biomechanical properties, instant shear modulus (IM), and equilibrium shear modulus (EM) of articular cartilage were investigated using an in situ indentation creep technique. The local variations in the concentration of glycosaminoglycans (GAGs) in the cartilage were measured with a microspectrophotometer after safranin-O staining of histological sections. Using a computer-based quantitative polarized light microscopy method, area-specific measurements of the optical path difference were performed to quantitate collagen-related optical retardation (gamma) of cartilage zones. The IM and EM were 131.3 and 51.2% higher (p < 0.001) in the femoral cartilage than in the tibial cartilage, respectively. The mean thickness of the superficial zone and the relative proportion of the superficial zone from the total uncalcified cartilage was 107.1 and 155.3% higher (p < 0.001) at the femoral test points than in the tibial ones, respectively. The mean thickness of the tibial uncalcified cartilage was 21.1% higher (p < 0.001) than the thickness of the femoral cartilage. The GAG concentration of the tibial cartilage was higher (14.8%, p < 0.001) than that of the femoral cartilage, especially in the superficial zone (50.0%, p < 0.05), whereas the gamma of the collagen network in the superficial zone of the femoral cartilage was 64.7% higher (p < 0.001) than in the tibial cartilage. The percent relative thickness and retardation gamma of the superficial zone correlated positively with the indentation stiffness of the canine knee articular cartilage. These observations indicate that cartilage is structurally inhomogenous and layered tissue and the local organization of collagen and GAG concentration of the articular cartilage regulate the biomechanical properties of the tissue. The structure and composition of the superficial articular cartilage significantly affects the indentation response of the canine knee articular cartilage.

[1]  I. Kiviranta,et al.  Moderate running exercise augments glycosaminoglycans and thickness of articular cartilage in the knee joint of young beagle dogs , 1988, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[2]  Van C. Mow,et al.  The Biomechanical Function of the Collagen Fibril Ultrastructure of Articular Cartilage , 1978 .

[3]  F. J. Dzida,et al.  Comparative study of the intrinsic mechanical properties of the human acetabular and femoral head cartilage , 1991, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[4]  C. R. Orford,et al.  Differential response to compressive loads of zones of canine hyaline articular cartilage: micromechanical, light and electron microscopic studies. , 1988, Annals of the rheumatic diseases.

[5]  H. Helminen,et al.  Local stimulation of proteoglycan synthesis in articular cartilage explants by dynamic compression in vitro , 1992, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[6]  J. Arokoski,et al.  Softening of the lateral condyle articular cartilage in the canine knee joint after long distance (up to 40 km/day) running training lasting one year. , 1994, International journal of sports medicine.

[7]  J Mizrahi,et al.  The "instantaneous" deformation of cartilage: effects of collagen fiber orientation and osmotic stress. , 1986, Biorheology.

[8]  E B Hunziker,et al.  Optical and mechanical determination of Poisson's ratio of adult bovine humeral articular cartilage. , 1997, Journal of biomechanics.

[9]  V C Mow,et al.  Variations in the intrinsic mechanical properties of human articular cartilage with age, degeneration, and water content. , 1982, The Journal of bone and joint surgery. American volume.

[10]  W C Hayes,et al.  Viscoelastic properties of human articular cartilage. , 1971, Journal of applied physiology.

[11]  B B Seedhom,et al.  Transmission of the Load in the Knee Joint with Special Reference to the Role of the Menisci Part I: Anatomy, Analysis and Apparatus , 1979 .

[12]  A F Mak,et al.  Viscoelastic properties of proteoglycan subunits and aggregates in varying solution concentrations. , 1984, Journal of biomechanics.

[13]  B. Weightman,et al.  Mechanical and biochemical properties of human articular cartilage in osteoarthritic femoral heads and in autopsy specimens. , 1986, The Journal of bone and joint surgery. British volume.

[14]  T. Brown,et al.  In vitro contact stress distribution on the femoral condyles , 1984, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[15]  G E Kempson,et al.  The effects of selective matrix degradation on the short-term compressive properties of adult human articular cartilage. , 1992, Biochimica et biophysica acta.

[16]  H J Helminen,et al.  Indentation study of the biochemical properties of articular cartilage in the canine knee. , 1987, Engineering in medicine.

[17]  B B Seedhom,et al.  Transmission of the Load in the Knee Joint with Special Reference to the Role of the Menisci: Part II: Experimental Results, Discussion and Conclusions , 1979 .

[18]  A. Maroudas,et al.  Measurement of swelling pressure in cartilage and comparison with the osmotic pressure of constituent proteoglycans. , 1981, Biorheology.

[19]  J. Buckwalter,et al.  Interspecies comparisons of in situ intrinsic mechanical properties of distal femoral cartilage , 1991, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[20]  H J Helminen,et al.  Biomechanical properties of the canine knee articular cartilage as related to matrix proteoglycans and collagen. , 1988, Engineering in medicine.

[21]  I. Kiviranta,et al.  Topographical variation of glycosaminoglycan content and cartilage thickness in canine knee (stifle) joint cartilage. Application of the microspectrophotometric method. , 1987, Journal of anatomy.

[22]  M. Freeman,et al.  Correlations between stiffness and the chemical constituents of cartilage on the human femoral head. , 1970, Biochimica et Biophysica Acta.

[23]  B B Seedhom,et al.  The stiffness of normal articular cartilage and the predominant acting stress levels: implications for the aetiology of osteoarthrosis. , 1993, British journal of rheumatology.

[24]  G. Kempson Age-related changes in the tensile properties of human articular cartilage: a comparative study between the femoral head of the hip joint and the talus of the ankle joint. , 1991, Biochimica et biophysica acta.

[25]  V C Mow,et al.  Tensile properties of human knee joint cartilage: I. Influence of ionic conditions, weight bearing, and fibrillation on the tensile modulus , 1986, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[26]  W M Lai,et al.  Fluid transport and mechanical properties of articular cartilage: a review. , 1984, Journal of biomechanics.

[27]  T Lapveteläinen,et al.  Decreased birefringence of the superficial zone collagen network in the canine knee (stifle) articular cartilage after long distance running training, detected by quantitative polarised light microscopy. , 1996, Annals of the rheumatic diseases.

[28]  V. Mow,et al.  Mechanical behavior of articular cartilage in shear is altered by transection of the anterior cruciate ligament , 1995, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.