Comparative effects of two multi-enzyme combinations and a Bacillus probiotic on growth performance, digestibility of energy and nutrients, disappearance of non-starch polysaccharides, and gut microflora in broiler chickens

&NA; The efficacy of two exogenous enzyme combinations and a multi‐strain Bacillus probiotic (DFM) on the growth performance, nutrient digestibility, disappearance of non‐starch polysaccharides (NSP) and gut microbial composition was investigated in broilers. One‐day old Ross 308 chicks were assigned to 36 pens with 22 birds/pen and 6 pens/treatment (Experiment 1) or 36 cages with 8 birds/cage and 6 cages/treatment (Experiment 2). Treatment additives were added to nutritionally complete corn/soy based starter (d 1 to 21) and finisher (d 22 to 42) diets. Treatments included 1) a control diet containing 500 FTU/kg phytase (CTL), 2) CTL + xylanase (2,000 U/kg) and amylase (200 U/kg; XA), 3) CTL+XA + protease (4000 U/g; XAP), 4) CTL+DFM (150,000 cfu/g of 3 strains of Bacillus spp), 5) CTL+DFM+XA, and 6) CTL+DFM+XAP. Supplementation with DFM increased BW, BWG, and FI compared with the CTL (P < 0.05); XAP, but not XA, resulted in increased final BW, BWG and FI compared to the control (P < 0.05). XA and XAP improved apparent ileal digestibility (AID) of starch and fat on d 22 to 42 with XAP improving AMEn (by ˜82 kcal) compared with CTL birds (P < 0.01). DFM+XAP improved apparent ileal digestible energy (AIDE), AID of fat and starch on d 22 to 42, and additionally had a greater than additive effect on AIDE and AMEn. Supplementation with DFM+XAP reduced the ileal and total tract flow of insoluble arabinose and additionally total tract flow of soluble and insoluble xylose and total galactose (P < 0.05); similar effects of XA+DFM were not seen or were lower in magnitude, suggesting that the protease component plays an important role in increasing the availability of NSP for hydrolysis. Supplementation with DFM alone did not affect gut bacterial populations, but XA and XAP reduced numbers of Campylobacter species (by > 2.5 log cfu/g; P < 0.001) and Bacteroides (P < 0.02) in the cecum compared with CTL birds.

[1]  A. Wealleans,et al.  Performance, gut morphology and microbiology effects of a Bacillus probiotic, avilamycin and their combination in mixed grain broiler diets , 2017, British poultry science.

[2]  V. Ravindran,et al.  Effect of exogenous xylanase, amylase, and protease as single or combined activities on nutrient digestibility and growth performance of broilers fed corn/soy diets , 2016, Poultry science.

[3]  A. Awati,et al.  Direct-fed microbial and its combination with xylanase, amylase, and protease enzymes in comparison with AGPs on broiler growth performance and foot-pad lesion development , 2016 .

[4]  O. Olukosi,et al.  Metabolizable energy content of wheat distillers' dried grains with solubles supplemented with or without a mixture of carbohydrases and protease for broilers and turkeys. , 2015, Poultry science.

[5]  S. Dalsgaard,et al.  Contribution of protein, starch, and fat to the apparent ileal digestible energy of corn- and wheat-based broiler diets in response to exogenous xylanase and amylase without or with protease1 , 2014, Poultry science.

[6]  V. Ravindran,et al.  Growth performance, nutrient utilization, and digesta characteristics in broiler chickens fed corn or wheat diets without or with supplemental xylanase. , 2014, Poultry science.

[7]  R M Gous,et al.  Modeling as a research tool in poultry science. , 2014, Poultry science.

[8]  E. Kiarie,et al.  The role of added feed enzymes in promoting gut health in swine and poultry , 2013, Nutrition Research Reviews.

[9]  P. Utterback,et al.  Comparative effects of dietary carbohydrases without or with protease on the ileal digestibility of energy and amino acids and AMEn in young broilers , 2013 .

[10]  A. Awati,et al.  A direct fed microbial containing a combination of three-strain Bacillus sp. can be used as an alternative to feed antibiotic growth promoters in broiler production , 2013 .

[11]  A. Cowieson,et al.  Exogenous enzymes and their effects on intestinal microbiology , 2012 .

[12]  S. Ricke,et al.  Direct-Fed Microbials and Prebiotics for Animals: Science and Mechanisms of Action , 2012 .

[13]  A. Cowieson,et al.  BOARD-INVITED REVIEW: opportunities and challenges in using exogenous enzymes to improve nonruminant animal production. , 2011, Journal of animal science.

[14]  Z. Kruk,et al.  Nutritive value of corn distillers dried grains with solubles as an ingredient of poultry diets: A review , 2010 .

[15]  U. Babu,et al.  Effects of direct-fed microbials on growth performance, gut morphometry, and immune characteristics in broiler chickens. , 2010, Poultry science.

[16]  W. Horwitz,et al.  Official methods of analysis of AOAC International , 2010 .

[17]  Pat Baird,et al.  Health benefits of dietary fiber. , 2009, Nutrition reviews.

[18]  V. Ravindran,et al.  Influence of dietary electrolyte balance and microbial phytase on growth performance, nutrient utilization, and excreta quality of broiler chickens. , 2008, Poultry science.

[19]  E. Topp,et al.  Development of a rapid quantitative PCR assay for direct detection and quantification of culturable and non-culturable Escherichia coli from agriculture watersheds. , 2007, Journal of microbiological methods.

[20]  K. Rudi,et al.  Comparison of chicken gut colonisation by the pathogens Campylobacter jejuni and Clostridium perfringens by real-time quantitative PCR. , 2006, Molecular and cellular probes.

[21]  V. Ravindran,et al.  Apparent ileal digestibility of amino acids in dietary ingredients for broiler chickens , 2005 .

[22]  A. Palva,et al.  Development of an extensive set of 16S rDNA‐targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real‐time PCR , 2004, Journal of applied microbiology.

[23]  Mogens Madsen,et al.  Detection of Campylobacter spp. in ChickenFecal Samples by Real-TimePCR , 2004, Journal of Clinical Microbiology.

[24]  B. Deplancke,et al.  Molecular ecological analysis of porcine ileal microbiota responses to antimicrobial growth promoters. , 2003, Journal of animal science.

[25]  D. Newell,et al.  Sources of Campylobacter Colonization in Broiler Chickens , 2003, Applied and Environmental Microbiology.

[26]  M. Gracia,et al.  Alpha-amylase supplementation of broiler diets based on corn. , 2003, Poultry science.

[27]  A. Chwalibog.  Substrate oxidation and retention in pigs and poultry , 2002 .

[28]  A. Eyigor,et al.  Implementation of real‐time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry , 2002, Letters in applied microbiology.

[29]  Katharine G. Field,et al.  A PCR Assay To Discriminate Human and Ruminant Feces on the Basis of Host Differences in Bacteroides-Prevotella Genes Encoding 16S rRNA , 2000, Applied and Environmental Microbiology.

[30]  M. Beck,et al.  The effect of dietary wheat middlings and enzyme supplementation II: apparent nutrient digestibility, digestive tract size, gut viscosity, and gut morphology in two strains of leghorn hens. , 1999, Poultry science.

[31]  M. Bedford,et al.  Exogenous enzymes for pigs and poultry , 1998, Nutrition Research Reviews.

[32]  J. Wiseman,et al.  Determination of titanium dioxide added as an inert marker in chicken digestibility studies , 1996 .

[33]  G. Pesti.  Nutrient requirements of poultry , 1995 .

[34]  M. Quigley,et al.  Determination of dietary fibre as non-starch polysaccharides with gas-liquid chromatographic, high-performance liquid chromatographic or spectrophotometric measurement of constituent sugars. , 1994, The Analyst.

[35]  S A McEwen,et al.  Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. , 1992, Molecular and cellular probes.

[36]  M. Choct,et al.  Anti-nutritive activities of cereal non-starch polysaccharides in broiler diets and strategies minimizing their effects , 1991 .

[37]  The health benefits of dietary fibre. , 1991, Nursing RSA = Verpleging RSA.

[38]  K. Leske,et al.  Effect of oligosaccharide-free soybean meal on true metabolizable energy and fiber digestion in adult roosters. , 1990, Poultry science.

[39]  J. J. Rackis,et al.  Determination of the total pepsin-pancreatin indigestible content (dietary fiber) of soybean products, wheat bran, and corn bran. , 1979, Journal of agricultural and food chemistry.

[40]  F. Hill,et al.  Comparison of metabolizable energy and productive energy determinations with growing chicks. , 1958, The Journal of nutrition.