Genetic Engineering Techniques in Fruit Science

Today, fruit science have well been established in world trade networks and sophisticated cultural and postharvest technologies that allow fruits to be enjoyed throughout much of the year, instead of mere weeks per year like our ancestors experienced. Especially modern biotechnological methods including genetic engineering technologies have been taken part in breeding strategies of fruit crops. Several biotechnological methods can be applied to plant to have better ones in the process of fruit breeding. Genetic engineering is a powerful tool for plant improvement and has the potential to allow the integration of desirable characteristics into existing genomes. Transformation technology developed a path to transfer important genes into plant genome for enhancing resistance against fungal, viral pathogens, other pests, drought, and salinity as well as silencing undesirable genes and improvement in nutrient acquisition. Different gene transfer techniques could be employed for fruit species. As well as direct and indirect transformation, modern genome editing methods recently have been used in plant science. In this review, we illustrated how to use these technologies in fruit science. Keywords— Agrocbacterium, direct gene transfer, CRISPR-CAS9, TALENs, ZFN.

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