The effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on rat testicular function

Wireless mobile phones and other telecommunication devices are used extensively in daily life. We therefore examined the effects of combined exposure to radiofrequency electromagnetic fields (RF-EMF) on rat testicular function, specifically with respect to sensitive processes such as spermatogenesis. Male rats were exposed to single code division multiple access (CDMA) and wideband code division multiple access (WCDMA) RF signals for 12 weeks. The RF exposure schedule comprised 45 min/day, 5 days/week for a total of 12 weeks. The whole-body average specific absorption rate (SAR) of CDMA and WCDMA was 2.0 W/kg each or 4.0 W/kg in total. We then investigated the correlates of testicular function such as sperm count in the cauda epididymis, testosterone concentration in the blood serum, malondialdehyde concentrations in the testes and epididymis, frequency of spermatogenesis stages, and appearance of apoptotic cells in the testes. We also immunoblotted for p53, bcl2, GADD45, cyclin G, and HSP70 in the testes of sham- and combined RF-exposed animals. Based on the results, we concluded that simultaneous exposure to CDMA and WCDMA RF-EMFs at 4.0 W/kg SAR did not have any observable adverse effects on rat spermatogenesis.

[1]  T. Okura,et al.  Troglitazone induces apoptosis via the p53 and Gadd45 pathway in vascular smooth muscle cells. , 2000, European journal of pharmacology.

[2]  A. Khadartsev,et al.  Effect of low-intensity extremely high frequency radiation on reproductive function in Wistar rats , 2006, Bulletin of Experimental Biology and Medicine.

[3]  Ashok Agarwal,et al.  Effect of cell phone usage on semen analysis in men attending infertility clinic: an observational study. , 2008, Fertility and sterility.

[4]  A. Ersay,et al.  Whole-body microwave exposure emitted by cellular phones and testicular function of rats , 1999, Urological Research.

[5]  Fehmi Ozguner,et al.  Biological and morphological effects on the reproductive organ of rats after exposure to electromagnetic field. , 2005, Saudi medical journal.

[6]  David Brusick,et al.  Genotoxicity of radiofrequency radiation , 1998, Environmental and molecular mutagenesis.

[7]  C. Rhoden,et al.  Effects of subchronic exposure to radio frequency from a conventional cellular telephone on testicular function in adult rats. , 2007, The Journal of urology.

[8]  H. Matloub,et al.  Effects of cellular phone emissions on sperm motility in rats. , 2007, Fertility and sterility.

[9]  G. Priante,et al.  Stage-specific expression of Gadd45 induced by X-irradiation in rat spermatogenesis , 2002, International journal of radiation biology.

[10]  L. Simmons,et al.  Image content influences men's semen quality , 2005, Biology Letters.

[11]  S F Cleary,et al.  Absorbed energy distribution from radiofrequency electromagnetic radiation in a mammalian cell model: effect of membrane-bound water. , 1995, Bioelectromagnetics.

[12]  Andreas Karabarbounis,et al.  Effect of GSM 900-MHz Mobile Phone Radiation on the Reproductive Capacity of Drosophila melanogaster , 2004 .

[13]  A. Pál,et al.  IS THERE A RELATIONSHIP BETWEEN CELL PHONE USE AND SEMEN QUALITY? , 2005, Archives of andrology.

[14]  C. Prives,et al.  A role of cyclin G in the process of apoptosis , 1999, Oncogene.

[15]  M. Bansal,et al.  Effect of oxidative stress on the spermatogenic process and hsp70 expressions in mice testes. , 2003, Indian journal of biochemistry & biophysics.

[16]  R. Hess,et al.  Quantitative and qualitative characteristics of the stages and transitions in the cycle of the rat seminiferous epithelium: light microscopic observations of perfusion-fixed and plastic-embedded testes. , 1990, Biology of reproduction.

[17]  D. McRee,et al.  Reproduction in male Japanese quail exposed to microwave radiation during embryogeny. , 1983, Radiation research.

[18]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[19]  E. Ulukaya,et al.  Mobile phone exposure does not induce apoptosis on spermatogenesis in rats. , 2008, Archives of medical research.

[20]  R. Kapp,et al.  An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals. A report of the U.S. Environmental Protection Agency Gene-Tox Program. , 1983, Mutation research.

[21]  Jeong-Ki Pack,et al.  One-year, simultaneous combined exposure of CDMA and WCDMA radiofrequency electromagnetic fields to rats , 2011, International journal of radiation biology.

[22]  K. Sarge,et al.  Regulation of hsp expression during rodent spermatogenesis , 1997, Cellular and Molecular Life Sciences CMLS.

[23]  A. Gautam,et al.  Demonstration of sperm head shape abnormality and clastogenic potential of cypermethrin. , 2004, Journal of environmental biology.

[24]  Osman Erogul,et al.  Effects of electromagnetic radiation from a cellular phone on human sperm motility: an in vitro study. , 2006, Archives of medical research.

[25]  P. Mathur,et al.  Lindane induces testicular apoptosis in adult Wistar rats through the involvement of Fas-FasL and mitochondria-dependent pathways. , 2009, Toxicology.

[26]  K. G. Adiyodi,et al.  Spermatogenesis and sperm function , 1983 .

[27]  J. Paick.  Role of Reactive Oxygen Species in Male Infertility , 2003 .

[28]  A. Giwercman,et al.  Identifying environmental risk to male reproductive function by occupational sperm studies: logistics and design options. , 1996, Occupational and environmental medicine.

[29]  E. Sheiner,et al.  Effect of occupational exposures on male fertility: literature review. , 2003, Industrial health.