Growth of the adult goldfish eye. II. Increase in retinal cell number

The retinas of adult goldfish, one to four years of age, 4–23 cm in length, were examined with standard paraffin histology to determine if new cells were being added with growth. Retinal cell nuclei were counted and the area of the retina was measured. An analysis of cell densities in various regions throughout the retina showed that the cells are distributed nearly homogeneously. The density (No./mm2 of retinal surface) of ganglion cells, inner nuclear layer cells and cones decreases with growth, but the density of rods remains constant. Thus the rods account for a larger proportion of the cells in larger retinas. The total number of cells per retina increases: the ganglion cells from 60,000 to 350,000; the inner nuclear layer cells from 1,500,000 to 4,000,000; the cones from 250,000 to 1,400,000; the rods from 1,500,000 to 15,000,000. This increase in the number of retinal neurons implies the formation of even more new synapses, and suggests the adult goldfish retina as a model for both neuro‐ and synaptogenesis.

[1]  T. Tamura.  A study of visual perception in fish, especially on resolving power and accommodation , 1957 .

[2]  A. Coulombre,et al.  Correlations of structural and biochemical changes in the developing retina of the chick. , 1955, The American journal of anatomy.

[3]  J. Blaxter,et al.  The Development of the Retina and Retinomotor Responses in the Herring , 1967, Journal of the Marine Biological Association of the United Kingdom.

[4]  M. Murray,et al.  Changes in the morphology and amino acid incorporation of regenerating goldfish optic neurons. , 1969, Experimental neurology.

[5]  G. L. Walls,et al.  The Vertebrate Eye and Its Adaptive Radiation. , 2013 .

[6]  G. M. Hope,et al.  Goldfish retina structure and function in extended cold. , 1971, Experimental neurology.

[7]  J. Blaxter.  The Eyes of Larval Fish , 1975 .

[8]  C. P. O'connell.  The structure of the eye of Sardinops caerulea, Engraulis mordax, and four other pelagic marine teleosts , 1963, Journal of morphology.

[9]  P. R. Johns.  Growth of the adult goldfish eye. III. Source of the new retinal cells , 1977, The Journal of comparative neurology.

[10]  J. Silver,et al.  The role of cell death during morphogenesis of the mammalian eye , 1973, Journal of morphology.

[11]  R. A. Weale,et al.  The Vertebrate Retina. Principles of Structure and Function , 1974 .

[12]  Ida Mann,et al.  The Development of the Human Eye , 1928, Nature.

[13]  M. Wilson.  Optic nerve fibre counts and retinal ganglion cell counts during development of Xenopus laevis (Daudin). , 1971, Quarterly journal of experimental physiology and cognate medical sciences.

[14]  B. Konigsmark,et al.  Methods for counting neurons , 1970 .

[15]  William K. Stell,et al.  Cone structure and visual pigment content in the retina of the goldfish , 1976, Vision Research.

[16]  H. O. Schwassmann.  Refractive State, Accommodation, and Resolving Power of the Fish Eye , 1975 .

[17]  J. Northrop,et al.  The chemistry and physiology of growth , 1949 .

[18]  G. K. Smelser.  The Vertebrate Eye and Its Adaptive Radiation. , 1943 .

[19]  O. A. Trowell,et al.  A method for differential cell counting in certain organs , 1959, The Anatomical record.

[20]  A. Glücksmann,et al.  DEVELOPMENT AND DIFFERENTIATION OF THE TADPOLE EYE , 1940, The British journal of ophthalmology.

[21]  F. Hester.  Visual contrast thresholds of the goldfish (Carassius auratus). , 1968, Vision research.

[22]  S. Easter,et al.  Growth of the adult goldfish eye—I: Optics , 1977, Vision Research.

[23]  R. Marc,et al.  The chromatic organization of the goldfish cone mosaic , 1976, Vision Research.

[24]  M. Abercrombie.  Estimation of nuclear population from microtome sections , 1946, The Anatomical record.