A label-free electrochemical DNA biosensor based on covalent immobilization of salmonella DNA sequences on the nanoporous glassy carbon electrode.

Herein, an easy and cost-effective approach to the immobilization of probe was performed. The amino modified salmonella ssDNA probe sequence was covalently linked with carboxylic group on the surface of nanoporous glassy carbon electrode to prepare the DNA biosensor. The differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques were used for the determination of salmonella DNA in the concentration ranges of 10-400pM and 1-400pM with limits of detection of 2.1pM and 0.15pM, respectively.

[1]  Xingyong Xu,et al.  A novel protocol for covalent immobilization of thionine on glassy carbon electrode and its application in hydrogen peroxide biosensor. , 2010, Biosensors & bioelectronics.

[2]  Takehiko Kitamori,et al.  Microbead-based rolling circle amplification in a microchip for sensitive DNA detection. , 2010, Lab on a chip.

[3]  Wei Cheng,et al.  A colorimetric assay method for invA gene of Salmonella using DNAzyme probe self-assembled gold nanoparticles as single tag , 2014 .

[4]  Tao Yang,et al.  A DNA electrochemical sensor based on nanogold-modified poly-2,6-pyridinedicarboxylic acid film and detection of PAT gene fragment. , 2007, Analytical biochemistry.

[5]  Dan Wu,et al.  A novel label-free electrochemical immunosensor based on graphene and thionine nanocomposite , 2010 .

[6]  R. Scharff,et al.  Economic burden from health losses due to foodborne illness in the United States. , 2012, Journal of food protection.

[7]  K. Ikebukuro,et al.  Amperometric DNA sensor using the pyrroquinoline quinone glucose dehydrogenase-avidin conjugate. , 2002, Biosensors & bioelectronics.

[8]  Martin A M Gijs,et al.  Label-free detection of DNA with interdigitated micro-electrodes in a fluidic cell. , 2008, Lab on a chip.

[9]  M. Zhang,et al.  Surface manipulation for improving the sensitivity and selectivity of glassy carbon electrodes by electrochemical treatment. , 2009, Biosensors & bioelectronics.

[10]  M. Ligaj,et al.  Electrochemical DNA biosensor for the detection of pathogenic bacteria Aeromonas hydrophila , 2014 .

[11]  S. Admassie,et al.  Electrochemically pretreated glassy carbon electrode for electrochemical detection of fenitrothion in tap water and human urine , 2013 .

[12]  Kae Sato,et al.  Bead-based padlock rolling circle amplification for single DNA molecule counting. , 2013, Analytical biochemistry.

[13]  R. Yazdanparast,et al.  Development and evaluation of a novel nucleic acid sequence-based amplification method using one specific primer and one degenerate primer for simultaneous detection of Salmonella Enteritidis and Salmonella Typhimurium. , 2013, Analytica chimica acta.

[14]  Kevin W Plaxco,et al.  Rapid, sequence-specific detection of unpurified PCR amplicons via a reusable, electrochemical sensor. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[15]  Tao Yang,et al.  Highly sensitive electrochemical impedance spectroscopic detection of DNA hybridization based on Au(nano)-CNT/PAN(nano) films. , 2009, Talanta.

[16]  Karel Vytras,et al.  Enzyme-Linked Electrochemical Detection of PCR-Amplified Nucleotide Sequences Using Disposable Screen-Printed Sensors. Applications in Gene Expression Monitoring , 2008, Sensors.

[17]  Wei Zhang,et al.  An enhanced sensing platform for ultrasensitive impedimetric detection of target genes based on ordered FePt nanoparticles decorated carbon nanotubes. , 2013, Biosensors & bioelectronics.

[18]  B. Haghighi,et al.  Direct electron transfer from glucose oxidase immobilized on a nano-porous glassy carbon electrode , 2011 .

[19]  Kuangwen Hsieh,et al.  Integrated microfluidic electrochemical DNA sensor. , 2009, Analytical chemistry.

[20]  Seunghun Hong,et al.  DNA sensors based on CNT-FET with floating electrodes , 2012 .

[21]  R. Engstrom,et al.  Characterization of electrochemically pretreated glassy carbon electrodes , 1984 .

[22]  R. Yazdanparast,et al.  Non-crosslinking gold nanoprobes for detection of nucleic acid sequence-based amplification products. , 2012, Analytical biochemistry.

[23]  M. S. Qureshi,et al.  A novel impedimetric biosensor based on graphene oxide/gold nanoplatform for detection of DNA arrays , 2013 .

[24]  Mehmet Lütfi Yola,et al.  A novel and sensitive electrochemical DNA biosensor based on Fe@Au nanoparticles decorated graphene oxide , 2014 .

[25]  M. Brémont,et al.  Veterinary Research is now a full Open Access journal , 2011, Veterinary research.

[26]  Meenakshi Choudhary,et al.  Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid , 2013 .

[27]  Li Niu,et al.  Green-synthesized gold nanoparticles decorated graphene sheets for label-free electrochemical impedance DNA hybridization biosensing. , 2011, Biosensors & bioelectronics.

[28]  D. Pang,et al.  Surface structure-related electrochemical behaviors of glassy carbon electrodes , 2008 .

[29]  A. Guadalupe,et al.  Synthesis, characterization and use of Ru-Fc intercalation complex as an electrochemical label for the detection of pathogen-DNA , 2010 .

[30]  C. Soeller,et al.  Conducting polymers for electrochemical DNA sensing. , 2009, Biomaterials.

[31]  V. Rai,et al.  Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp. , 2012, Talanta.

[32]  Ke-Jing Huang,et al.  A sensitive electrochemical DNA biosensor based on silver nanoparticles-polydopamine@graphene composite , 2014 .

[33]  Alessandra Bonanni,et al.  Genomagnetic assay based on label-free electrochemical detection using magneto-composite electrodes , 2006 .

[34]  C. Iversen,et al.  Inclusivity, exclusivity and limit of detection of commercially available real-time PCR assays for the detection of Salmonella. , 2013, International journal of food microbiology.

[35]  Yu Bai,et al.  Rapid and simultaneous detection of Salmonella, Shigella, and Staphylococcus aureus in fresh pork using a multiplex real-time PCR assay based on immunomagnetic separation , 2014 .

[36]  Bo Liedberg,et al.  Label-free, electrochemical detection of methicillin-resistant Staphylococcus aureus DNA with reduced graphene oxide-modified electrodes. , 2011, Biosensors & bioelectronics.

[37]  M. Wiedmann,et al.  Animal contact as a source of human non-typhoidal salmonellosis , 2011, Veterinary research.

[38]  Wei Cheng,et al.  Label-free and high-sensitive detection of Salmonella using a surface plasmon resonance DNA-based biosensor. , 2012, Journal of biotechnology.

[39]  C. Lei,et al.  Electrochemical immobilization of horseradish peroxidase on an electro-activated glassy carbon electrode , 1996 .