Simultaneous determination of iodide and creatinine in human urine by flow analysis with an on-line sample treatment column.

This work presents the first flow system for direct analysis of iodide and creatinine suitable for screening of human urine samples. The system had a mini-column packed with strong anion exchange resin for on-line extraction of iodide. After injection of a sample on the column the unretained urine sample was analyzed for creatinine in one section of the flow system using the Jaffe's reaction with spectrometric detection at 520 nm. Iodide was eluted off with 1.42 mL 5 M NaNO3. A 150 μL fraction of the eluate was analyzed in another section of the same flow system for iodide using the kinetic-spectrometric Sandell-Kolthoff reaction. At the optimum condition, the sample throughput was 12 samples per h. The linear working range covered the normal levels of iodide and creatinine in human urine: 0-200 μg I L(-1) and 50-1200 mg creatinine L(-1), respectively. Recoveries tested in 10 samples were 87-104% for iodide and 89-104% for creatinine. Bland-Altman plots (n = 50) showed that the scatter of the differences between values obtained by this method and those of reference methods, for both iodide and creatinine, was within mean ± 2SD.

[1]  Haiying Chen,et al.  Headspace single-drop microextraction coupled with gas chromatography electron capture detection of butanone derivative for determination of iodine in milk powder and urine , 2013, Chemical Papers.

[2]  S. Kapur,et al.  Iodine and creatinine testing in urine dried on filter paper. , 2013, Analytica chimica acta.

[3]  Fang Zeng,et al.  Carbon dots-based fluorescent probes for sensitive and selective detection of iodide , 2013, Microchimica Acta.

[4]  V. Piersoel,et al.  Urine iodide determination by ion-pair reversed-phase high performance liquid chromatography and pulsed amperometric detection. , 2012, Talanta.

[5]  E. Favaloro,et al.  A robust method for testing urinary iodine using a microtitre robotic system. , 2011, Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements.

[6]  M. Zimmermann,et al.  Ten repeat collections for urinary iodine from spot samples or 24-hour samples are needed to reliably estimate individual iodine status in women. , 2011, Journal of NutriLife.

[7]  P. Dasgupta,et al.  Review of analytical methods for the quantification of iodine in complex matrices. , 2011, Analytica chimica acta.

[8]  M. Hedayati,et al.  Rapid microwave digestion and microplate reading format method for urinary iodine determination , 2011, Clinical chemistry and laboratory medicine.

[9]  S. Kanno,et al.  Determination of iodide in urine using electrospray ionization tandem mass spectrometry. , 2010, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[10]  A. Melse-Boonstra,et al.  Iodine deficiency in pregnancy, infancy and childhood and its consequences for brain development. , 2010, Best practice & research. Clinical endocrinology & metabolism.

[11]  P. Jooste,et al.  Methods for determination of iodine in urine and salt. , 2010, Best practice & research. Clinical endocrinology & metabolism.

[12]  B. de Benoist,et al.  Epidemiology of iodine deficiency: Salt iodisation and iodine status. , 2010, Best practice & research. Clinical endocrinology & metabolism.

[13]  T. Jørgensen,et al.  Estimation of iodine intake from various urinary iodine measurements in population studies. , 2009, Thyroid : official journal of the American Thyroid Association.

[14]  Adisorn Tuantranont,et al.  Portable microfluidic system for determination of urinary creatinine. , 2009, Analytica chimica acta.

[15]  C. Pandav,et al.  Iodine-deficiency disorders , 2008, The Lancet.

[16]  B. Michalke,et al.  Method developments for iodine speciation by reversed-phase liquid chromatography-ICP-mass spectrometry , 2007, Biological Trace Element Research.

[17]  W. M. Wan Nazaimoon,et al.  A cost-effective modified micromethod for measuring urine iodine. , 2006, Tropical biomedicine.

[18]  D. Nacapricha,et al.  Use of pseudo-first order kinetics in flow injection for determination of trace inorganic iodine , 2003 .

[19]  D. Nacapricha,et al.  Continuous and stopped flow injection for catalytic determination of total iodine in urine. , 2001, The Analyst.

[20]  C. Pandav,et al.  Simple microplate method for determination of urinary iodine. , 2000, Clinical chemistry.

[21]  B. Michalke,et al.  Iodine speciation in biological samples by capillary electrophoresis ‐ inductively coupled plasma mass spectrometry , 1999, Electrophoresis.

[22]  L. Ovesen,et al.  Day-to-day and within-day variation in urinary iodine excretion , 1999, European Journal of Clinical Nutrition.

[23]  L. Braverman,et al.  Ammonium persulfate: a new and safe method for measuring urinary iodine by ammonium persulfate oxidation. , 2009, Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association.

[24]  C. Huiqin,et al.  Spectrophotometric determination of urinary iodine by flow-injection analysis with on-line catalytic digestion. , 1996, Clinical Chemistry.

[25]  L. Braverman,et al.  Ammonium persulfate: a safe alternative oxidizing reagent for measuring urinary iodine. , 1996, Clinical chemistry.

[26]  S. Nagataki,et al.  Automated measurement of urinary iodine with use of ultraviolet irradiation. , 1995, Clinical chemistry.

[27]  A. D. Dunn,et al.  Two simple methods for measuring iodine in urine. , 1993, Thyroid : official journal of the American Thyroid Association.

[28]  H. Ford,et al.  Ascorbic acid interferes with an automated urinary iodide determination based on the ceric-arsenious acid reaction. , 1991, Clinical chemistry.

[29]  C. Eastman,et al.  Evaluation of automated urinary iodine methods: problems of interfering substances identified. , 1990, Clinical chemistry.

[30]  J. Tressol,et al.  Improved routine method for the determination of total iodine in urine and milk. , 1986, The Analyst.

[31]  H. Taussky,et al.  ON THE COLORIMETRIC DETERMINATION OF CREATININE BY THE JAFFE REACTION , 1945 .

[32]  I. Kolthoff,et al.  CHRONOMETRIC CATALYTIC METHOD FOR THE DETERMINATION OF MICRO QUANTITIES OF IODINE , 1934 .