Prospective Respiration-Gated Photoacoustic Microscopy

Objective: Photoacoustic microscopy (PAM) is a promising biomedical imaging technique that relies on sequential excitation to generate three-dimensional images. It combines the high contrast of optical imaging with high penetration depth of ultrasound imaging. The normal respiration rate of mice is greater than 3 Hz, which leads to motion artifacts in most reported PAM for in vivo imaging. Methods: Here, we introduce a prospective respiratory gating (PRG) method for PAM to address this problem. We captured the mouse's respiratory signal with a laser displacement sensor, and the photoacoustic signal was acquired at specific phase points of the respiratory signal. The scanning mode and the scanning timing were also designed and evaluated. We combined this method with our PAM to demonstrate its feasibility. Results: Our experiments show that the proposed method can help remove motion artifacts well, and the subcutaneous vascular imaging results of the mouse abdominal region with PRG are much better than those without any gating.

[1]  Junjie Yao,et al.  Label-free oxygen-metabolic photoacoustic microscopy in vivo. , 2011, Journal of biomedical optics.

[2]  Michael J. Moore,et al.  Single Cell Photoacoustic Microscopy: A Review , 2016, IEEE Journal of Selected Topics in Quantum Electronics.

[3]  L. Hedlund,et al.  Micro-CT with respiratory and cardiac gating. , 2004, Medical physics.

[4]  Qingming Luo,et al.  Fast axial-scanning photoacoustic microscopy using tunable acoustic gradient lens. , 2017, Optics express.

[5]  Jun Zou,et al.  In vivo label-free photoacoustic flow cytography and on-the-spot laser killing of single circulating melanoma cells , 2016, Scientific Reports.

[6]  Alejandro Garcia-Uribe,et al.  Video-rate functional photoacoustic microscopy at depths , 2012, Journal of biomedical optics.

[7]  James A Bankson,et al.  Improved method of in vivo respiratory-gated micro-CT imaging , 2004, Physics in medicine and biology.

[8]  Qingming Luo,et al.  Reflection-mode Bessel-beam photoacoustic microscopy for in vivo imaging of cerebral capillaries. , 2016, Optics express.

[9]  J Dinkel,et al.  Fully automated intrinsic respiratory and cardiac gating for small animal CT , 2010, Physics in medicine and biology.

[10]  Gordon D. Love,et al.  High-resolution 3D optical microscopy inside the beating zebrafish heart using prospective optical gating , 2012, Biomedical optics express.

[11]  T H Farncombe.  Software-based respiratory gating for small animal conebeam CT. , 2008, Medical physics.

[12]  Ralph Weissleder,et al.  Imaging the beating heart in the mouse using intravital microscopy techniques , 2015, Nature Protocols.

[13]  Lihong V. Wang,et al.  Photoacoustic imaging and characterization of the microvasculature. , 2010, Journal of biomedical optics.

[14]  Junjie Yao,et al.  Photoacoustic microscopy with enhanced resolution and imaging depth aided by optical clearing , 2014, Photonics West - Biomedical Optics.

[15]  Lihong V. Wang,et al.  Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging , 2006, Nature Biotechnology.

[16]  Zhuang Liu,et al.  Carbon nanotubes as photoacoustic molecular imaging agents in living mice. , 2008, Nature nanotechnology.

[17]  Ralph Weissleder,et al.  Sequential average segmented microscopy for high signal-to-noise ratio motion-artifact-free in vivo heart imaging. , 2013, Biomedical optics express.

[18]  Junjie Yao,et al.  Multiscale Functional and Molecular Photoacoustic Tomography , 2016, Ultrasonic imaging.

[19]  Qingming Luo,et al.  Self-Assembled "Off/On" Nanopomegranate for In Vivo Photoacoustic and Fluorescence Imaging: Strategic Arrangement of Kupffer Cells in Mouse Hepatic Lobules. , 2019, ACS nano.

[20]  Mathieu Pernot,et al.  3-D real-time motion correction in high-intensity focused ultrasound therapy. , 2004, Ultrasound in medicine & biology.

[21]  Gang Zheng,et al.  Stomach wall structure and vessels imaging by acoustic resolution photoacoustic microscopy , 2018, World journal of gastroenterology.

[22]  Yueh Z. Lee,et al.  Non-contact respiration monitoring for in-vivo murine micro computed tomography: characterization and imaging applications , 2012, Physics in medicine and biology.

[23]  Lihong V. Wang,et al.  High-speed label-free functional photoacoustic microscopy of mouse brain in action , 2015, Nature Methods.

[24]  Lihong V. Wang,et al.  Biomedical Optics: Principles and Imaging , 2007 .

[25]  Lihong V. Wang,et al.  Single-cell label-free photoacoustic flowoxigraphy in vivo , 2013, Proceedings of the National Academy of Sciences.

[26]  Konstantin Maslov,et al.  Retrospective respiration-gated whole-body photoacoustic computed tomography of mice , 2014, Journal of biomedical optics.

[27]  Maria Drangova,et al.  Prospective respiratory-gated micro-CT of free breathing rodents. , 2005, Medical physics.

[28]  Xinmai Yang,et al.  Growth of melanoma brain tumors monitored by photoacoustic microscopy. , 2010, Journal of biomedical optics.

[29]  Junjie Yao,et al.  Single-impulse Panoramic Photoacoustic Computed Tomography of Small-animal Whole-body Dynamics at High Spatiotemporal Resolution , 2017, Nature Biomedical Engineering.