DIVERSITY OF ARTHRODERMATACEAE COMMUNITIES THAT CREATE HAVOC TO THE OVERALL HEALTH OF HUMAN AND ANIMALS

Keratinous substance rich soil is most conducive for keratinophilic fungi growth and occurrence. Dermatophytes and other related fungi are potential pathogens causing human and animal dermatomycoses. Herein, this study went for screening the presence of dermatophytes and related keratinophilic fungi in various soil samples collected from different locations of Sharkia Governorate, Egypt. A total of 80 soil samples from roadsides, fields and stables were subjected for mycological analysis using modified hair-bait technique with hair of horse, cattle and goat, sheep wool, and chicken feathers as a keratin source for keratinophilic fungi growth. Keratinophilic fungi were identified according to their phenotypical characterization in combination with PCR amplification and sequencing for internal transcribed spacer (ITS) region of rDNA. Keratinophilic fungi were recovered from 73.75% of soil samples (59/80). Field soils yielded a higher positivity rate for keratinophilic fungal isolates (90%) than roadsides (66.67%) and stables (58.82%). The majority of keratinophilic fungi belonged to dermatophytes (57.47%). Microsporum gypseum (50.85%) were detected in the majority of sites followed by Trichophyton mentagrophytes (30.51%), Chrysosporium species (28.81%), C. keratinophilium (23.73%), C. tropicum, C. zonatum, Arthroderma multifidum , Arthroderma benhami , Arthroderma fulvum , Clonostachys species, Simplicillium obclavatum and Purpureocillium lilacinum (1.69%, each). It was found that horse and goat hair were more suitable for isolation of keratinophilic fungi with a percentage of 100% for each, followed by cattle hair (91.66%), sheep wool (87.5%) and chicken feathers (83.33%). This investigation demonstrated that the various soils of Sharkia Governorate might be critical suppliers of certain keratinophilic fungi that may constitute hazards to human and animal health. The genetic-based identification is strongly recommended for a high discriminatory identification of keratinophilic fungi. Key words: keratinophilic fungi; geophilic dermatophytes; Arthroderma benhami ; Arthroderma fulvum; ITS sequencing

[1]  M. Taha,et al.  Yeast species associated with diseased fish: Occurrence, identification, experimental challenges and antifungal susceptibility testing , 2018 .

[2]  Y. Mahmmod,et al.  Species Identification, Strain Differentiation, and Antifungal Susceptibility of Dermatophyte Species Isolated From Clinically Infected Arabian Horses , 2017 .

[3]  F. Hassan,et al.  Assessment of carvacrol for control of avian aspergillosis in intratracheally challenged chickens in comparison to voriconazole with a reference on economic impact , 2017, Journal of applied microbiology.

[4]  H. Kamata,et al.  First Isolation of Arthroderma fulvum in Japan. , 2017, Medical mycology journal.

[5]  Y. Chavan,et al.  Incidence of Keratinophilic Fungi from the Selected Soils of Kaziranga National Park, Assam (India) , 2016, Mycopathologia.

[6]  Y. Mahmmod,et al.  Diagnostic performance of molecular and conventional methods for identification of dermatophyte species from clinically infected Arabian horses in Egypt. , 2016, Veterinary dermatology.

[7]  J. Zimmerman,et al.  Pulmonary Infection Secondary to Chrysosporium zonatum in an Immunocompetent Man. , 2016, Annals of the American Thoracic Society.

[8]  L. Xi,et al.  Epidemiology of Superficial Fungal Infections in Guangdong, Southern China: A Retrospective Study from 2004 to 2014 , 2016, Mycopathologia.

[9]  E. Meler,et al.  Disseminated Chrysosporium infection in a German shepherd dog , 2015, Medical mycology case reports.

[10]  S. Ranque,et al.  Distribution of Keratinophilic Fungi in Soil Across Tunisia: A Descriptive Study and Review of the Literature , 2015, Mycopathologia.

[11]  S. Deshmukh,et al.  Isolation of keratinophilic fungi from selected soils of Sanjay Gandhi National Park, Mumbai (India). , 2014, Journal de mycologie medicale.

[12]  D. Ferreira,et al.  Diversity of Clonostachys species assessed by molecular phylogenetics and MALDI-TOF mass spectrometry. , 2014, Fungal biology.

[13]  U. Hipler,et al.  Trichophyton species of Arthroderma benhamiae – a new infectious agent in dermatology , 2014, Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG.

[14]  Y. Terashima,et al.  Isolation of Dermatophytes and Related Species from Domestic Fowl (Gallus gallus domesticus) , 2014, Mycopathologia.

[15]  P. Venkatesh,et al.  Endogenous Endophthalmitis by Chrysosporium: An Opportunistic Pathogen , 2014, Ocular immunology and inflammation.

[16]  D. Otranto,et al.  Molecular epidemiology, phylogeny and evolution of dermatophytes. , 2013, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[17]  Koichiro Tamura,et al.  MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. , 2013, Molecular biology and evolution.

[18]  Y. Tartor,et al.  Use of Fourier transform infrared spectroscopy (FTIR) spectroscopy for rapid and accurate identification of Yeasts isolated from human and animals , 2013 .

[19]  S. Kaul,et al.  Polymerase Chain Reaction: Restriction Fragment Length Polymorphism Differentiates the Environmental and Clinically Important Fungal Isolates , 2013, National Academy Science Letters.

[20]  S. Ōmura,et al.  Five new Simplicillium species (Cordycipitaceae) from soils in Tokyo, Japan , 2013 .

[21]  Rama,et al.  A Rare Case of Nasal Septal Perforation Due to Purpureocillium lilacinum: Case Report and Review , 2013, Indian Journal of Otolaryngology and Head & Neck Surgery.

[22]  S. Anane.  Epidemiological investigation of keratinophilic fungi from soils of Djerba (Tunisia). , 2012, Journal de mycologie medicale.

[23]  J. Novák,et al.  Keratinophilic Fungi Isolated from Soils of Long-Term Fold-Grazed, Degraded Pastures in National Parks of Slovakia , 2012, Mycopathologia.

[24]  S. Deshmukh,et al.  Prevalence of keratinophilic fungi in public park soils of Mumbai, India , 2012 .

[25]  R. Kachuei,et al.  Isolation of keratinophilic fungi from soil in Isfahan province, Iran. , 2012, Journal de mycologie medicale.

[26]  J. Bohacz,et al.  Species diversity of keratinophilic fungi in various soil types , 2012, Central European Journal of Biology.

[27]  J. Graybill,et al.  Adiaspiromycosis Causing Respiratory Failure and a Review of Human Infections Due to Emmonsia and Chrysosporium spp , 2012, Journal of Clinical Microbiology.

[28]  J. Houbraken,et al.  Purpureocillium, a new genus for the medically important Paecilomyces lilacinus. , 2011, FEMS microbiology letters.

[29]  Y. Sharma,et al.  PATHOGENESIS OF DERMATOPHYTOSES , 2011, Indian journal of dermatology.

[30]  Meenakshi Sharma,et al.  Distribution of dermatophytes and other related fungi in Jaipur city, with particular reference to soil pH , 2011, Mycoses.

[31]  S. Teerthanath,et al.  A Rare Case of Fungal Maxillary Sinusitis due to Paecilomyces lilacinus in an Immunocompetent Host, Presenting as a Subcutaneous Swelling , 2011, Journal of laboratory physicians.

[32]  R. Kushwaha,et al.  Antagonism of Microsporum species by soil fungi , 2010, Mycoses.

[33]  A. Ramis,et al.  Cutaneous hyalohyphomycosis caused by a Chrysosporium species related to Nannizziopsis vriesii in two green iguanas (Iguana iguana). , 2008, Medical mycology.

[34]  Thanaa A. Maghraby,et al.  Keratinophilic fungi inhabiting floor dusts of student houses at the South Valley University in Egypt , 2008 .

[35]  Q. Mandeel,et al.  Keratinophilic fungi from selected soils of Bahrain , 2008, Mycopathologia.

[36]  Y. A. Youssef,et al.  Keratinophilic Fungi Recovered from Muddy Soil in Cairo Vicinities, Egypt , 2005, Mycopathologia.

[37]  S. Deshmukh.  Incidence of dermatophytes and other keratinophilic fungi in the glacier bank soils of the Kashmir valley, India , 2002 .

[38]  T. White.  Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics , 1990 .

[39]  G. F. Orr.  Kerationophilic fungi isolated from soils by a modified hair bait technique. , 1969, Sabouraudia.

[40]  C. Suh.  Studies on Baiting Methods for Isolation of Keratinophilic Fungi. , 1966 .