Directed molecular evolution to design advanced red fluorescent proteins
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[1] Joerg Bewersdorf,et al. Far-red fluorescent protein excitable with red lasers for flow cytometry and superresolution STED nanoscopy. , 2010, Biophysical journal.
[2] Michael Z. Lin,et al. Autofluorescent proteins with excitation in the optical window for intravital imaging in mammals. , 2009, Chemistry & biology.
[3] Bernd Rieger,et al. One‐ and two‐photon photoactivation of a paGFP‐fusion protein in live Drosophila embryos , 2005, FEBS letters.
[4] Y. Cho,et al. Development of GFP-based biosensors possessing the binding properties of antibodies , 2009, Proceedings of the National Academy of Sciences.
[5] W. Telford,et al. Supercontinuum white light lasers for flow cytometry , 2009, Cytometry. Part A : the journal of the International Society for Analytical Cytology.
[6] Bin Wu,et al. Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics. , 2011, Current opinion in cell biology.
[7] Vladislav V Verkhusha,et al. Engineering ESPT pathways based on structural analysis of LSSmKate red fluorescent proteins with large Stokes shift. , 2010, Journal of the American Chemical Society.
[8] Mark A Naivar,et al. Digital analysis and sorting of fluorescence lifetime by flow cytometry , 2010, Cytometry. Part A : the journal of the International Society for Analytical Cytology.
[9] Dmitriy M Chudakov,et al. Conversion of red fluorescent protein into a bright blue probe. , 2008, Chemistry & biology.
[10] M. Drobizhev,et al. Two-photon absorption properties of fluorescent proteins , 2011, Nature Methods.
[11] Xin Heng,et al. Serial line scan encoding imaging cytometer for both adherent and suspended cells. , 2011, Analytical chemistry.
[12] S. Lukyanov,et al. Fluorescent proteins and their applications in imaging living cells and tissues. , 2010, Physiological reviews.
[13] Aaron R Wheeler,et al. A microfluidic platform for complete mammalian cell culture. , 2010, Lab on a chip.
[14] D. Xing,et al. Micropumps, microvalves, and micromixers within PCR microfluidic chips: Advances and trends. , 2007, Biotechnology advances.
[15] Andreas Martin,et al. Proside: a phage-based method for selecting thermostable proteins. , 2003, Methods in molecular biology.
[16] Alexander S. Mishin,et al. Green fluorescent proteins are light-induced electron donors , 2009, Nature chemical biology.
[17] W. Xu,et al. Microtable arrays for culture and isolation of cell colonies , 2010, Analytical and bioanalytical chemistry.
[18] Suliana Manley,et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy , 2009, Nature Methods.
[19] Brenda M Ogle,et al. Multiphoton Flow Cytometry to Assess Intrinsic and Extrinsic Fluorescence in Cellular Aggregates: Applications to Stem Cells , 2010, Microscopy and Microanalysis.
[20] C. Tyler-Smith,et al. Attenuation of green fluorescent protein half-life in mammalian cells. , 1999, Protein engineering.
[21] A. Wlodawer,et al. Understanding blue-to-red conversion in monomeric fluorescent timers and hydrolytic degradation of their chromophores. , 2010, Journal of the American Chemical Society.
[22] V. Malashkevich,et al. Photoactivation mechanism of PAmCherry based on crystal structures of the protein in the dark and fluorescent states , 2009, Proceedings of the National Academy of Sciences of the United States of America.
[23] John S. Condeelis,et al. A photoswitchable orange-to-far-red fluorescent protein, PSmOrange , 2011, Nature Methods.
[24] G Ulrich Nienhaus,et al. Structure, dynamics and optical properties of fluorescent proteins: perspectives for marker development. , 2009, Chemphyschem : a European journal of chemical physics and physical chemistry.
[25] Travis J Gould,et al. Superresolution imaging of multiple fluorescent proteins with highly overlapping emission spectra in living cells. , 2011, Biophysical journal.
[26] M. Davidson,et al. Engineered fluorescent proteins: innovations and applications , 2009, Nature Methods.
[27] F. Cava,et al. Thermus thermophilus as biological model , 2009, Extremophiles.
[28] A. N. van den Pol,et al. Viral Mutagenesis as a Means for Generating Novel Proteins , 2009, Journal of Virology.
[29] N. D. Da Silva,et al. Relationship between thermal stability, degradation rate and expression yield of barnase variants in the periplasm of Escherichia coli. , 1996, Protein engineering.
[30] M. Davidson,et al. A monomeric photoconvertible fluorescent protein for imaging of dynamic protein localization. , 2010, Journal of molecular biology.
[31] P. Daugherty. Protein engineering with bacterial display. , 2007, Current opinion in structural biology.
[32] Mark A. Naivar,et al. Single particle high resolution spectral analysis flow cytometry , 2006, Cytometry. Part A : the journal of the International Society for Analytical Cytology.
[33] Jitae Kim,et al. Cell lysis on a microfluidic CD (compact disc). , 2004, Lab on a chip.
[34] Vladislav V Verkhusha,et al. Monomeric fluorescent timers that change color from blue to red report on cellular trafficking. , 2009, Nature chemical biology.
[35] Roberto A Chica,et al. Generation of longer emission wavelength red fluorescent proteins using computationally designed libraries , 2010, Proceedings of the National Academy of Sciences.
[36] Stefan W. Hell,et al. A Rapidly Maturing Far-Red Derivative of DsRed-Express2 for Whole-Cell Labeling , 2009, Biochemistry.
[37] R. B. Caldwell,et al. Protein evolution by hypermutation and selection in the B cell line DT40 , 2007, Nucleic acids research.
[38] W. Zipfel,et al. Molecular Mechanism of a Green-Shifted, pH-Dependent Red Fluorescent Protein mKate Variant , 2011, PloS one.
[39] Konstantin A Lukyanov,et al. Red fluorescent protein with reversibly photoswitchable absorbance for photochromic FRET. , 2010, Chemistry & biology.
[40] Chun-Hao Chen,et al. Microfluidics and photonics for Bio‐System‐on‐a‐Chip: A review of advancements in technology towards a microfluidic flow cytometry chip , 2008, Journal of biophotonics.
[41] V. Verkhusha,et al. Advances in engineering of fluorescent proteins and photoactivatable proteins with red emission. , 2010, Current Opinion in Chemical Biology.
[42] Christian Eggeling,et al. Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins. , 2005, Proceedings of the National Academy of Sciences of the United States of America.
[43] Michael W. Davidson,et al. Photoconversion in orange and red fluorescent proteins , 2009, Nature Methods.
[44] J. Lippincott-Schwartz,et al. Photoactivatable fluorescent proteins for diffraction-limited and super-resolution imaging. , 2009, Trends in cell biology.
[45] Konstantin A Lukyanov,et al. The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore. , 2008, Biochemistry.
[46] J. Lippincott-Schwartz,et al. Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells. , 2010, Journal of the American Chemical Society.
[47] Belinda C. Ferrari,et al. Applications of flow cytometry in environmental microbiology and biotechnology , 2009, Extremophiles.
[48] V. Malashkevich,et al. Structural characterization of acylimine-containing blue and red chromophores in mTagBFP and TagRFP fluorescent proteins. , 2010, Chemistry & biology.
[49] J. Goedhart,et al. Bright cyan fluorescent protein variants identified by fluorescence lifetime screening , 2010, Nature Methods.
[50] The use of Cyan Fluorescent Protein variants with a distinctive lifetime signature. , 2009, Molecular bioSystems.