Utility of antibodies against a 22 kD molecule of Dirofilaria immitis in the diagnosis of human pulmonary dirofilariasis

Summary To assess the characteristics of an ELISA test for the diagnosis of human pulmonary dirofilariasis, we studied the sera of 24 subjects with other helmintoses and of 37 patients suffering from non‐parasitic focal lung diseases, comparing them with negative and positive sera. ELISA and Western blot with complete somatic antigen and ELISA with protein Di22 (specifically recognized in cases of lung dirofilariasis) were performed. With ELISA SA the false positive rate was 25% in cases with other parasitoses and 30% in cases with focal lung diseases. ELISA Di22 decreases this positivity levels. Only 2 cases with visceral larva migrans (8.3%) and a case with lung nodules metastatic from renal adenocarcinoma (2.7%) were positive. ELISA Di22 therefore greatly decreases the false positive rate of ELISA SA.

[1]  A. Muro,et al.  Evaluation of a 22 kDa Dirofilaria immitis antigen for the immunodiagnosis of human pulmonary dirofilariosis. , 1994, Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit.

[2]  M. Southworth,et al.  Expression of an Onchocerca volvulus Ov33 homolog in Dirofilaria immitis: potential in immunodiagnosis of heartworm infection , 1994, Parasite immunology.

[3]  S. Sun,et al.  Immunodiagnosis of human dirofilariasis by enzyme-linked immunosorbent assay using recombinant DNA-derived fusion protein , 1992, Journal of Helminthology.

[4]  P. Pairolero,et al.  Dirofilaria immitis: a rare, increasing cause of pulmonary nodules. , 1992, Mayo Clinic proceedings.

[5]  A. Muro,et al.  Small calcified nodule: an undescribed radiologic manifestation of human pulmonary dirofilariasis. , 1992, The Journal of infectious diseases.

[6]  A. Muro,et al.  A seroepidemiologic survey of human dirofilariosis in Western Spain. , 1991, Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit.

[7]  A. Muro,et al.  Transient solitary pulmonary nodule caused by Dirofilaria immitis. , 1990, The European respiratory journal.

[8]  A. M. Alvarez,et al.  Seroepidemiological studies on human pulmonary dirofilariasis in Spain. , 1990 .

[9]  A. M. Alvarez,et al.  Epidemiological studies on toxocariasis and visceral larva migrans in a zone of Western Spain , 1989 .

[10]  A. Muro Álvarez,et al.  Epidemiological studies on toxocariasis and visceral larva migrans in a zone of western Spain. , 1989, Annals of tropical medicine and parasitology.

[11]  A. Scott,et al.  Quantitative and qualitative changes in the humoral response of dogs through the course of infection with Dirofilaria immitis. , 1985, American Journal of Tropical Medicine and Hygiene.

[12]  F. Ciferri.  Human pulmonary dirofilariasis in the United States: a critical review. , 1982, The American journal of tropical medicine and hygiene.