No effect of antioxidant supplementation on muscle performance and blood redox status adaptations to eccentric training.

BACKGROUND It was recently reported that antioxidant supplementation decreases training efficiency and prevents cellular adaptations to chronic exercise. OBJECTIVE This study aimed to investigate the effects of vitamin C and vitamin E supplementation on muscle performance, blood and muscle redox status biomarkers, and hemolysis in trained and untrained men after acute and chronic exercise. A specific type of exercise was applied (eccentric) to produce long-lasting and extensive changes in redox status biomarkers and to examine more easily the potential effects of antioxidant supplementation. DESIGN In a double-blinded fashion, men received either a daily oral supplement of vitamin C and vitamin E (n = 14) or placebo (n = 14) for 11 wk (started 4 wk before the pretraining exercise testing and continued until the posttraining exercise testing). After baseline testing, the subjects performed an eccentric exercise session 2 times/wk for 4 wk. Before and after the chronic eccentric exercise, the subjects underwent one session of acute eccentric exercise, physiologic measurements were performed, and blood samples and muscle biopsy samples (from 4 men) were collected. RESULTS The results failed to support any effect of antioxidant supplementation. Eccentric exercise similarly modified muscle damage and performance, blood redox status biomarkers, and hemolysis in both the supplemented and nonsupplemented groups. This occurred despite the fact that eccentric exercise induced marked changes in muscle damage and performance and in redox status after exercise. CONCLUSION The complete lack of any effect on the physiologic and biochemical outcome measures used raises questions about the validity of using oral antioxidant supplementation as a redox modulator of muscle and redox status in healthy humans.

[1]  M. Jackson.  Muscle damage during exercise: possible role of free radicals and protective effect of vitamin E , 1987, Proceedings of the Nutrition Society.

[2]  A. Jamurtas,et al.  Eccentric exercise affects the upper limbs more than the lower limbs in position sense and reaction angle , 2010, Journal of sports sciences.

[3]  A. Jamurtas,et al.  Decreased blood oxidative stress after repeated muscle-damaging exercise. , 2007, Medicine and science in sports and exercise.

[4]  J. Sastre,et al.  Decreasing xanthine oxidase‐mediated oxidative stress prevents useful cellular adaptations to exercise in rats , 2005, The Journal of physiology.

[5]  T. Hurst,et al.  Oxidative stress, inflammation and recovery of muscle function after damaging exercise: effect of 6-week mixed antioxidant supplementation , 2011, European Journal of Applied Physiology.

[6]  D. Talwar,et al.  A routine method for the simultaneous measurement of retinol, alpha-tocopherol and five carotenoids in human plasma by reverse phase HPLC. , 1998, Clinica chimica acta; international journal of clinical chemistry.

[7]  A. Jamurtas,et al.  Comparison between glucose-6-phosphate dehydrogenase-deficient and normal individuals after eccentric exercise. , 2009, Medicine and science in sports and exercise.

[8]  J. Morrow,et al.  Effect of alpha-tocopherol supplementation on plasma homocysteine and oxidative stress in highly trained athletes before and after exhaustive exercise. , 2005, The Journal of nutritional biochemistry.

[9]  M. Falvo,et al.  Prior exercise and antioxidant supplementation: effect on oxidative stress and muscle injury , 2007, Journal of the International Society of Sports Nutrition.

[10]  J. Bergström.  Percutaneous Needle Biopsy of Skeletal Muscle in Physiological and Clinical Research , 1975 .

[11]  T. Shimomitsu,et al.  Vitamin E Supplementation and Endurance Exercise , 2000, Sports medicine.

[12]  A. Jamurtas,et al.  The Effect of Muscle-Damaging Exercise on Blood and Skeletal Muscle Oxidative Stress , 2008, Sports medicine.

[13]  W. Evans,et al.  Suction applied to a muscle biopsy maximizes sample size. , 1982, Medicine and science in sports and exercise.

[14]  R. Brigelius-Flohé,et al.  Vitamin E: a vitamin still awaiting the detection of its biological function. , 2010, Molecular nutrition & food research.

[15]  A. Jamurtas,et al.  Oxidative stress biomarkers responses to physical overtraining: implications for diagnosis. , 2007, Free radical biology & medicine.

[16]  Galin L. Jones,et al.  Supplemental vitamin C appears to slow racing greyhounds. , 2002, The Journal of nutrition.

[17]  D. Pascoe,et al.  Lymphocyte enzymatic antioxidant responses to oxidative stress following high-intensity interval exercise. , 2011, Journal of applied physiology.

[18]  C. Napoli,et al.  Chronic Antioxidant Supplementation Impairs Coronary Endothelial Function and Myocardial Perfusion in Normal Pigs , 2006, Hypertension.

[19]  A. Azzi.  Molecular mechanism of alpha-tocopherol action. , 2007, Free radical biology & medicine.

[20]  G. Lamb,et al.  Acute effects of reactive oxygen and nitrogen species on the contractile function of skeletal muscle , 2011, The Journal of physiology.

[21]  H. Poulsen,et al.  Is vitamin C supplementation beneficial? Lessons learned from randomised controlled trials , 2009, British Journal of Nutrition.

[22]  A. Jamurtas,et al.  Corrigendum to “Oxidative stress biomarkers responses to physical overtraining: Implications for diagnosis” [Free Radic. Biol. Med. 43 (2007) 901-910] , 2008 .

[23]  P. Jakemanl,et al.  Effect of antioxidant vitamin supplementation on muscle function after eccentric exercise , 2004, European Journal of Applied Physiology and Occupational Physiology.

[24]  B. Halliwell,et al.  Lack of effect of acute oral ingestion of vitamin C on oxidative stress, arterial stiffness or blood pressure in healthy subjects , 2008, Free radical research.

[25]  S. Basu,et al.  Supplementation with a combination of antioxidants does not affect glycaemic control, oxidative stress or inflammation in type 2 diabetes subjects , 2010, Free radical research.

[26]  B. Pedersen,et al.  Antioxidant supplementation does not alter endurance training adaptation. , 2010, Medicine and science in sports and exercise.

[27]  D. Bailey,et al.  Oral antioxidants and cardiovascular health in the exercise-trained and untrained elderly: a radically different outcome. , 2009, Clinical science.

[28]  S. Powers,et al.  Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. , 2008, Physiological reviews.

[29]  B. Halliwell,et al.  Free radicals in biology and medicine , 1985 .

[30]  L. Carrera-Quintanar,et al.  Effect of lemon verbena supplementation on muscular damage markers, proinflammatory cytokines release and neutrophils’ oxidative stress in chronic exercise , 2011, European Journal of Applied Physiology.

[31]  A. Azzi.  Molecular mechanism of α-tocopherol action , 2007 .

[32]  A. Jamurtas,et al.  Blood as a reactive species generator and redox status regulator during exercise. , 2009, Archives of biochemistry and biophysics.

[33]  B. Frei,et al.  Can antioxidant vitamins materially reduce oxidative damage in humans? , 1999, Free radical biology & medicine.

[34]  A. Berthelot,et al.  Dietary vitamin C supplementation decreases blood pressure in DOCA-salt hypertensive male Sprague Dawley rats and this is associated with increased liver oxidative stress , 2002, Molecular and Cellular Biochemistry.

[35]  J. Morrow.  Quantification of Isoprostanes as Indices of Oxidant Stress and the Risk of Atherosclerosis in Humans , 2004, Arteriosclerosis, thrombosis, and vascular biology.

[36]  J. Sastre,et al.  Oral administration of vitamin C decreases muscle mitochondrial biogenesis and hampers training-induced adaptations in endurance performance. , 2008, The American journal of clinical nutrition.

[37]  B. Pedersen,et al.  ROS and myokines promote muscle adaptation to exercise , 2009, Trends in Endocrinology & Metabolism.

[38]  D. Laaksonen,et al.  α-Lipoic acid modulates thiol antioxidant defences and attenuates exercise-induced oxidative stress in standardbred trotters , 2009, Free radical research.

[39]  Michael Stumvoll,et al.  Antioxidants prevent health-promoting effects of physical exercise in humans , 2009, Proceedings of the National Academy of Sciences.

[40]  D. Doran,et al.  Ascorbic acid supplementation does not attenuate post-exercise muscle soreness following muscle-damaging exercise but may delay the recovery process , 2006, British Journal of Nutrition.

[41]  G. FitzGerald,et al.  Effects of vitamin E on lipid peroxidation in healthy persons. , 2001, JAMA.

[42]  A. Donnelly,et al.  Effects of dietary supplementation with vitamins C and E on muscle function during and after eccentric contractions in humans , 2004, European Journal of Applied Physiology.

[43]  A. Jamurtas,et al.  A weekly bout of eccentric exercise is sufficient to induce health-promoting effects. , 2011, Medicine and science in sports and exercise.

[44]  Steven R Steinhubl,et al.  Why have antioxidants failed in clinical trials? , 2008, The American journal of cardiology.

[45]  J. Morrow,et al.  Vitamin E and immunity after the Kona Triathlon World Championship. , 2004, Medicine and science in sports and exercise.

[46]  M. Reid.  Free radicals and muscle fatigue: Of ROS, canaries, and the IOC. , 2008, Free radical biology & medicine.

[47]  R. Cutlip,et al.  Vitamin E and C supplementation reduces oxidative stress, improves antioxidant enzymes and positive muscle work in chronically loaded muscles of aged rats , 2010, Experimental Gerontology.

[48]  J. Brisswalter,et al.  Vitamin and mineral supplementation effect on muscular activity and cycling efficiency in master athletes. , 2010, Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme.