Robust and semi-interpenetrating hydrogels from poly(ethylene glycol) and collagen for elastomeric tissue scaffolds.

Here we present an injectable PEG/collagen hydrogel system with robust networks for use as elastomeric tissue scaffolds. Covalently crosslinked PEG and physically crosslinked collagen form semi-interpenetrating networks. The mechanical strength of the hydrogels depends predominantely on the PEG concentration but the incorporation of collagen into the PEG network enhances hydrogel viscoelasticity, elongation, and also cell adhesion properties. Experimental data show that this hydrogel system exhibits tunable mechanical properties that can be further developed. The hydrogels allow cell adhesion and proliferation in vitro. The results support the prospect of a robust and semi-interpenetrating biomaterial for elastomeric tissue scaffolds applications.

[1]  Aaron D Baldwin,et al.  Polysaccharide‐modified synthetic polymeric biomaterials , 2010, Biopolymers.

[2]  A. Fatimi,et al.  Hydrogels for Cartilage Tissue Engineering , 2010 .

[3]  Patrick J. Schexnailder,et al.  Nanocomposite polymer hydrogels , 2009 .

[4]  Ali Khademhosseini,et al.  Synthesis and characterization of tunable poly(ethylene glycol): gelatin methacrylate composite hydrogels. , 2011, Tissue engineering. Part A.

[5]  S. Bryant,et al.  Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels. , 2002, Journal of biomedical materials research.

[6]  T. Lodge,et al.  Hydrogels from ABA and ABC Triblock Polymers , 2010 .

[7]  Glenn D Prestwich,et al.  The generation of 3-D tissue models based on hyaluronan hydrogel-coated microcarriers within a rotating wall vessel bioreactor. , 2010, Biomaterials.

[8]  Jay C. Sy,et al.  Maleimide Cross‐Linked Bioactive PEG Hydrogel Exhibits Improved Reaction Kinetics and Cross‐Linking for Cell Encapsulation and In Situ Delivery , 2012, Advanced materials.

[9]  Atu Agawu,et al.  An in situ forming collagen-PEG hydrogel for tissue regeneration. , 2012, Acta biomaterialia.

[10]  Karyn G. Robinson,et al.  Differential effects of substrate modulus on human vascular endothelial, smooth muscle, and fibroblastic cells. , 2012, Journal of biomedical materials research. Part A.

[11]  J. West,et al.  Cell migration through defined, synthetic extracellular matrix analogues , 2002, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[12]  K. Anseth,et al.  Hydrogel Cell Cultures , 2007, Science.

[13]  Ali Khademhosseini,et al.  Mechanically robust and bioadhesive collagen and photocrosslinkable hyaluronic acid semi-interpenetrating networks. , 2009, Tissue engineering. Part A.

[14]  Jan C. M. van Hest,et al.  Peptide- and Protein-Based Hydrogels , 2012 .

[15]  Molly S. Shoichet,et al.  Polymer Scaffolds for Biomaterials Applications , 2010 .

[16]  A. Gaharwar,et al.  Mechanically Tough Pluronic F127/Laponite Nanocomposite Hydrogels from Covalently and Physically Cross-Linked Networks , 2011 .

[17]  D. Mooney,et al.  Hydrogels for tissue engineering: scaffold design variables and applications. , 2003, Biomaterials.

[18]  Kinam Park,et al.  pH-sensitivity of fast responsive superporous hydrogels , 2000, Journal of biomaterials science. Polymer edition.

[19]  J. Gong,et al.  Double Network Hydrogels as Tough, Durable Tissue Substitutes , 2010 .

[20]  Peter X Ma,et al.  Biomimetic materials for tissue engineering. , 2008, Advanced drug delivery reviews.

[21]  Eunhee Cho,et al.  The effect of hyaluronic acid incorporation on fibroblast spreading and proliferation within PEG-diacrylate based semi-interpenetrating networks. , 2007, Biomaterials.

[22]  Rong Jin,et al.  Hydrogels for Tissue Engineering Applications , 2010 .

[23]  W. Hennink,et al.  Hydrogels as extracellular matrices for skeletal tissue engineering: state-of-the-art and novel application in organ printing. , 2007, Tissue engineering.

[24]  D E Ingber,et al.  Cellular control lies in the balance of forces. , 1998, Current opinion in cell biology.

[25]  J. West,et al.  Vascularization of engineered tissues: approaches to promote angio-genesis in biomaterials. , 2008, Current topics in medicinal chemistry.

[26]  Edorta Santos,et al.  Novel advances in the design of three-dimensional bio-scaffolds to control cell fate: translation from 2D to 3D. , 2012, Trends in biotechnology.

[27]  Jason A Burdick,et al.  Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering. , 2002, Biomaterials.

[28]  Jeffrey A. Hubbell,et al.  Bioerodible hydrogels based on photopolymerized poly(ethylene glycol)-co-poly(.alpha.-hydroxy acid) diacrylate macromers , 1993 .

[29]  D. Kelly,et al.  Mechanically induced structural changes during dynamic compression of engineered cartilaginous constructs can potentially explain increases in bulk mechanical properties , 2012, Journal of The Royal Society Interface.

[30]  M. Pishko,et al.  Release of protein from highly cross-linked hydrogels of poly(ethylene glycol) diacrylate fabricated by UV polymerization. , 2001, Biomaterials.

[31]  Wen-Fu Lee,et al.  Studies on preparation and swelling properties of the N‐isopropylacrylamide/chitosan semi‐IPN and IPN hydrogels , 2001 .

[32]  Francesco M Veronese,et al.  PEGylation, successful approach to drug delivery. , 2005, Drug discovery today.

[33]  Jennifer L West,et al.  Cell adhesion peptides alter smooth muscle cell adhesion, proliferation, migration, and matrix protein synthesis on modified surfaces and in polymer scaffolds. , 2002, Journal of biomedical materials research.

[34]  J. Elisseeff,et al.  Photoencapsulation of chondrocytes in poly(ethylene oxide)-based semi-interpenetrating networks. , 2000, Journal of biomedical materials research.

[35]  Akhilesh K Gaharwar,et al.  Transparent, elastomeric and tough hydrogels from poly(ethylene glycol) and silicate nanoparticles. , 2011, Acta biomaterialia.

[36]  Laurence A. Heinrich,et al.  Micromechanical Properties of “Smart” Gels: Studies by Scanning Force and Scanning Electron Microscopy of PNIPAAm , 2002 .

[37]  Kytai Truong Nguyen,et al.  Photopolymerizable hydrogels for tissue engineering applications. , 2002, Biomaterials.

[38]  J. van der Gucht,et al.  Macromolecular Diffusion in Self-Assembling Biodegradable Thermosensitive Hydrogels. , 2010, Macromolecules.

[39]  Seon Jeong Kim,et al.  Synthesis and characteristics of interpenetrating polymer network hydrogel composed of chitosan and poly(acrylic acid) , 1999 .

[40]  S. Sen,et al.  Matrix Elasticity Directs Stem Cell Lineage Specification , 2006, Cell.

[41]  K. Pal,et al.  Polymeric Hydrogels: Characterization and Biomedical Applications , 2009 .

[42]  V. Guarino,et al.  Composite Hydrogels for Scaffold Design, Tissue Engineering, and Prostheses , 2010 .

[43]  Xinqiao Jia,et al.  Hybrid multicomponent hydrogels for tissue engineering. , 2009, Macromolecular bioscience.

[44]  A. Khademhosseini,et al.  Hydrogels in Biology and Medicine: From Molecular Principles to Bionanotechnology , 2006 .

[45]  Shyni Varghese,et al.  PEG/clay nanocomposite hydrogel: a mechanically robust tissue engineering scaffold , 2010 .

[46]  N. Peppas,et al.  Morphology of poly(methacrylic acid)/poly(N-isopropyl acrylamide) interpenetrating polymeric networks , 2002, Journal of biomaterials science. Polymer edition.

[47]  Antonios G Mikos,et al.  Injectable Biomaterials for Regenerating Complex Craniofacial Tissues , 2009, Advanced materials.

[48]  Wim E. Hennink,et al.  Novel crosslinking methods to design hydrogels , 2002 .

[49]  Jyrki Heino,et al.  Integrin-mediated Cell Adhesion to Type I Collagen Fibrils* , 2004, Journal of Biological Chemistry.

[50]  Ehud Gazit,et al.  Self-assembled peptide nanostructures: the design of molecular building blocks and their technological utilization. , 2007, Chemical Society reviews.

[51]  Robert Langer,et al.  Stimulation of neurite outgrowth by neurotrophins delivered from degradable hydrogels. , 2006, Biomaterials.

[52]  C. Chu,et al.  Pore structure analysis of swollen dextran-methacrylate hydrogels by SEM and mercury intrusion porosimetry. , 2000, Journal of biomedical materials research.

[53]  A. Gaharwar,et al.  Highly extensible, tough, and elastomeric nanocomposite hydrogels from poly(ethylene glycol) and hydroxyapatite nanoparticles. , 2011, Biomacromolecules.

[54]  L. Schmidt‐Mende,et al.  ZnO - nanostructures, defects, and devices , 2007 .

[55]  Giyoong Tae,et al.  Formulation and in vitro characterization of an in situ gelable, photo-polymerizable Pluronic hydrogel suitable for injection. , 2007, Journal of controlled release : official journal of the Controlled Release Society.

[56]  M. Grinstaff,et al.  Applications of dendrimers in tissue engineering. , 2008, Current topics in medicinal chemistry.