Real-time quantitative phase imaging based on transport of intensity equation with dual simultaneously recorded field of view.

Since quantitative phase distribution reflects both cellular shapes and conditions from another view, compared to traditional intensity observation, different quantitative phase microscopic methods are proposed for cellular detections. However, the transport of intensity equation-based approach not only presents phase, but also intensity, which attracts much attention. While classical transport of intensity equation needs multi-focal images which often cannot realize simultaneous phase measurement, in this Letter, to break through the limitation, a real-time quantitative phase imaging method using transport of intensity equation is proposed. Two identical CCD cameras are set at the binocular tubes to capture the same field of view but at different focal planes. With a double-frame algorithm assuming that the on-focal image is the average of over- and under-focal information, the proposed method is capable of calculating quantitative phase distributions of samples accurately and simultaneously indicating its potentialities in cellular real-time monitoring.

[1]  A. Asundi,et al.  Noninterferometric single-shot quantitative phase microscopy. , 2013, Optics letters.

[2]  Pietro Ferraro,et al.  Gravity driven high throughput phase detecting cytometer based on quantitative interferometric microscopy , 2014 .

[3]  Pinhas Girshovitz,et al.  Generalized cell morphological parameters based on interferometric phase microscopy and their application to cell life cycle characterization , 2012, Biomedical optics express.

[4]  A. Asundi,et al.  High-speed transport-of-intensity phase microscopy with an electrically tunable lens. , 2013, Optics express.

[5]  K. Nugent,et al.  Rapid quantitative phase imaging using the transport of intensity equation , 1997 .

[6]  P. Marquet,et al.  Automated statistical quantification of three-dimensional morphology and mean corpuscular hemoglobin of multiple red blood cells. , 2012, Optics express.

[7]  G. Barbastathis,et al.  Transport of intensity phase imaging in a volume holographic microscope. , 2010, Optics letters.

[8]  Jinli Suo,et al.  Gerchberg-Saxton-like ghost imaging. , 2015, Optics express.

[9]  J. Rodenburg,et al.  A phase retrieval algorithm for shifting illumination , 2004 .

[10]  L. Tian,et al.  Transport of Intensity phase-amplitude imaging with higher order intensity derivatives. , 2010, Optics express.

[11]  Lei Tian,et al.  Compressive x-ray phase tomography based on the transport of intensity equation. , 2013, Optics letters.

[12]  G. Barbastathis,et al.  Phase imaging for absorptive phase objects using hybrid uniform and structured illumination transport of intensity equation. , 2014, Optics express.

[13]  L. Tian,et al.  Nonlinear diffusion regularization for transport of intensity phase imaging. , 2012, Optics letters.

[14]  K. Nugent,et al.  Quantitative optical phase microscopy. , 1998, Optics letters.

[15]  R. Gerchberg.  A practical algorithm for the determination of phase from image and diffraction plane pictures , 1972 .

[16]  D. Polyzos,et al.  Scattering of he-ne laser light by an average-sized red blood cell. , 1999, Applied optics.

[17]  M. Teague.  Deterministic phase retrieval: a Green’s function solution , 1983 .

[18]  Rene A. Claus,et al.  Transport of Intensity phase imaging by intensity spectrum fitting of exponentially spaced defocus planes. , 2014, Optics express.

[19]  L. Tian,et al.  Low-noise phase imaging by hybrid uniform and structured illumination transport of intensity equation. , 2014, Optics express.

[20]  Yibo Zhang,et al.  Wide-field computational imaging of pathology slides using lens-free on-chip microscopy , 2014, Science Translational Medicine.

[21]  R. Horstmeyer,et al.  Wide-field, high-resolution Fourier ptychographic microscopy , 2013, Nature Photonics.

[22]  Qian Chen,et al.  Transport-of-intensity phase imaging using Savitzky-Golay differentiation filter--theory and applications. , 2013, Optics express.

[23]  S. S. Gorthi,et al.  Phase imaging flow cytometry using a focus-stack collecting microscope. , 2012, Optics letters.

[24]  Sung-Hee Hong,et al.  Characterizations of individual mouse red blood cells parasitized by Babesia microti using 3-D holographic microscopy , 2015, Scientific Reports.

[25]  G. Truskey,et al.  Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry. , 2011, Journal of biomedical optics.

[26]  Gabriel Popescu,et al.  Quantitative Phase Imaging , 2012 .

[27]  L. Tian,et al.  The transport of intensity equation for optical path length recovery using partially coherent illumination. , 2013, Optics express.

[28]  G. Barbastathis,et al.  Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging. , 2010, Optics letters.

[29]  Pasquale Memmolo,et al.  3D morphometry of red blood cells by digital holography , 2014, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[30]  Qionghai Dai,et al.  Content adaptive illumination for Fourier ptychography. , 2014, Optics letters.

[31]  Youngchan Kim,et al.  Common-path diffraction optical tomography for investigation of three-dimensional structures and dynamics of biological cells. , 2014, Optics express.

[32]  Chao Zuo,et al.  Iterative optimum frequency combination method for high efficiency phase imaging of absorptive objects based on phase transfer function. , 2015, Optics express.

[33]  Victoria J Allan,et al.  Light Microscopy Techniques for Live Cell Imaging , 2003, Science.