"DMS," a computer-assisted system for quantitating motility, the dynamics of cytoplasmic flow, and pseudopod formation: its application to Dictyostelium chemotaxis.

A computer-assisted Dynamic Morphology System (DMS) is described that allows the rapid quantitation of more than 30 parameters of motility and dynamic morphology for up to 40 amebae in parallel. This system also generates "difference pictures" for characterizing the dynamics of pseudopod formation. A 3-D DMS is described, and application of DMS to problems of motility and chemotaxis in normal and mutant cells of Dictyostelium discoideum is reviewed.

[1]  D R Soll,et al.  “Dynamic morphology system”: A method for quantitating changes in shape, pseudopod formation, and motion in normal and mutant amoebae of Dictyostelium discoideum , 1988, Journal of cellular biochemistry.

[2]  D. Soll,et al.  Chemoresponsiveness to cAMP and folic acid during growth, development, and dedifferentiation in Dictyostelium discoideum. , 1981, Differentiation; research in biological diversity.

[3]  R. Macnab,et al.  The gradient-sensing mechanism in bacterial chemotaxis. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[4]  E. Voss,et al.  Amebae of Dictyostelium discoideum respond to an increasing temporal gradient of the chemoattractant cAMP with a reduced frequency of turning: evidence for a temporal mechanism in ameboid chemotaxis. , 1987, Cell motility and the cytoskeleton.

[5]  H. Berg,et al.  Chemotaxis in Escherichia coli analysed by Three-dimensional Tracking , 1972, Nature.

[6]  J. Douglas Faires,et al.  Numerical Analysis , 1981 .

[7]  D R Soll,et al.  Frequency and orientation of pseudopod formation of Dictyostelium discoideum amebae chemotaxing in a spatial gradient: further evidence for a temporal mechanism. , 1987, Cell motility and the cytoskeleton.

[8]  W. Loomis,et al.  Cell motility and chemotaxis in Dictyostelium amebae lacking myosin heavy chain. , 1988, Developmental biology.

[9]  D A Knecht,et al.  Antisense RNA inactivation of myosin heavy chain gene expression in Dictyostelium discoideum. , 1987, Science.

[10]  J. Spudich,et al.  Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination. , 1987, Science.

[11]  D. Soll,et al.  The developmental regulation of single-cell motility in Dictyostelium discoideum. , 1986, Developmental biology.

[12]  D. Soll,et al.  Dictyostelium amebae alter motility differently in response to increasing versus decreasing temporal gradients of cAMP , 1985, The Journal of cell biology.

[13]  Zigmond Sh.  Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors. , 1977 .

[14]  D. Soll,et al.  Adaptation in the motility response to cAMP in Dictyostelium discoideum. , 1988, Cell motility and the cytoskeleton.

[15]  P. Devreotes.  CHAPTER 4 – Chemotaxis , 1982 .