Antibiofilm activity of nanosized magnesium fluoride.

The ability of bacteria to develop antibiotic resistance and colonize abiotic surfaces by forming biofilms is a major cause of medical implant-associated infections and results in prolonged hospitalization periods and patient mortality. This raises the urgent need to develop compounds that can inhibit bacterial colonization of surfaces. In this study, we present an unreported microwave-based synthesis of MgF(2) nanoparticles (Nps) using ionic liquid. We demonstrate the antimicrobial activity of these fluoride nanomaterials and their ability to restrict biofilm formation of common bacterial pathogens. Scanning and transmission electron microscopic techniques indicated that the MgF(2).Nps attach and penetrate into the cells. Flow cytometry analysis revealed that the Nps caused a disruption in the membrane potential. The MgF(2).Nps also induced membrane lipid peroxidation and once internalized can interact with chromosomal DNA. Based on these findings we further explored the possibility of using the MgF(2).Nps to coat surfaces and inhibit biofilm formation. A microwave synthesis and coating procedure was utilized to coat glass coupons. The MgF(2) coated surfaces effectively restricted biofilm formation of the tested bacteria. Taken together these results highlight the potential for developing MgF(2) nanoparticles in order to inhibit bacterial infections.

[1]  Zichuan Ma,et al.  Efficient disinfection of Escherichia coli in water by silver loaded alumina , 2008, Journal of Inorganic Biochemistry.

[2]  S. Croft.  Methods in Molecular Biology; Electron Microscopy Methods and Protocols, Vol. 117; M.A. Nasser Hajibagheri (Ed.); Humana Press, 1999, 296 pages, Comb bound, ISBN 0-89603-640-5, US $89.50 , 2001 .

[3]  S. Ghosh,et al.  Green fluorescent protein-expressing Escherichia coli as a model system for investigating the antimicrobial activities of silver nanoparticles. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[4]  J. Costerton,et al.  Bacterial biofilms: a common cause of persistent infections. , 1999, Science.

[5]  Anand Gole,et al.  Targeted photothermal lysis of the pathogenic bacteria, Pseudomonas aeruginosa, with gold nanorods. , 2008, Nano letters.

[6]  R. E. Marquis,et al.  Fluoride and organic weak acids as modulators of microbial physiology. , 2003, FEMS microbiology reviews.

[7]  A. Manna,et al.  Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. , 2008, FEMS microbiology letters.

[8]  A. Gedanken,et al.  Are Ionic Liquids Really a Boon for the Synthesis of Inorganic Materials? A General Method for the Fabrication of Nanosized Metal Fluorides , 2006 .

[9]  M. Benedetti,et al.  Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium. , 2006, Nano letters.

[10]  Roberto Kolter,et al.  Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis , 1998, Molecular microbiology.

[11]  K. Klabunde,et al.  Metal Oxide Nanoparticles as Bactericidal Agents , 2002 .

[12]  Howard M. Shapiro,et al.  Multiparameter Flow Cytometric Analysis of Antibiotic Effects on Membrane Potential, Membrane Permeability, and Bacterial Counts of Staphylococcus aureus andMicrococcus luteus , 2000, Antimicrobial Agents and Chemotherapy.

[13]  I. Sondi,et al.  Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. , 2004, Journal of colloid and interface science.

[14]  Franck Chauvat,et al.  Cytotoxicity of CeO2 nanoparticles for Escherichia coli. Physico-chemical insight of the cytotoxicity mechanism. , 2006, Environmental science & technology.

[15]  B. Gibbins,et al.  Antimicrobial surface functionalization of plastic catheters by silver nanoparticles. , 2008, The Journal of antimicrobial chemotherapy.

[16]  B. Goldstein,et al.  Mutagenicity of malonaldehyde, a decomposition product of peroxidized polyunsaturated fatty acids. , 1976, Science.

[17]  R. Darouiche,et al.  Treatment of infections associated with surgical implants. , 2004, The New England journal of medicine.

[18]  Á. Catala.  Lipid peroxidation of membrane phospholipids generates hydroxy-alkenals and oxidized phospholipids active in physiological and/or pathological conditions. , 2009, Chemistry and physics of lipids.

[19]  S. Cooper,et al.  Bacterial colonization of functionalized polyurethanes. , 2000, Biomaterials.

[20]  D. Davies,et al.  Understanding biofilm resistance to antibacterial agents , 2003, Nature Reviews Drug Discovery.

[21]  C. Sissons,et al.  Inhibition of purified enolases from oral bacteria by fluoride. , 1997, Oral microbiology and immunology.

[22]  J. Song,et al.  Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli , 2007, Applied and Environmental Microbiology.

[23]  K. Neoh,et al.  Antibacterial and mechanical properties of bone cement impregnated with chitosan nanoparticles. , 2006, Biomaterials.

[24]  M. Sturr,et al.  Inhibition of proton-translocating ATPases of Streptococcus mutans and Lactobacillus casei by fluoride and aluminum , 2004, Archives of Microbiology.

[25]  Abraham J Domb,et al.  Surface antimicrobial activity and biocompatibility of incorporated polyethylenimine nanoparticles. , 2008, Biomaterials.

[26]  J. Costerton,et al.  Bacterial biofilms: a diagnostic and therapeutic challenge , 2003, Expert review of anti-infective therapy.

[27]  Raz Jelinek,et al.  Microwave‐Assisted Synthesis of Nanocrystalline MgO and Its Use as a Bacteriocide , 2005 .

[28]  R. Chemaly,et al.  Anti-adherence activity and antimicrobial durability of anti-infective-coated catheters against multidrug-resistant bacteria. , 2008, The Journal of antimicrobial chemotherapy.

[29]  Sung Ju Cho,et al.  Unmodified cadmium telluride quantum dots induce reactive oxygen species formation leading to multiple organelle damage and cell death. , 2005, Chemistry & biology.

[30]  Pedro J J Alvarez,et al.  Fullerene water suspension (nC60) exerts antibacterial effects via ROS-independent protein oxidation. , 2008, Environmental science & technology.

[31]  Paul Stoodley,et al.  Bacterial biofilms: from the Natural environment to infectious diseases , 2004, Nature Reviews Microbiology.

[32]  R. Kolter,et al.  Biofilm formation as microbial development. , 2000, Annual review of microbiology.

[33]  D. Bonnefont-Rousselot,et al.  [Biomarkers of lipid peroxidation: analytical aspects]. , 2008, Annales de biologie clinique.

[34]  D. A. Brown,et al.  Implications and potential applications of bactericidal fullerene water suspensions: effect of nC(60) concentration, exposure conditions and shelf life. , 2008, Water science and technology : a journal of the International Association on Water Pollution Research.

[35]  L. Marnett.  Lipid peroxidation-DNA damage by malondialdehyde. , 1999, Mutation research.

[36]  R. E. Marquis.  Antimicrobial actions of fluoride for oral bacteria. , 1995, Canadian journal of microbiology.

[37]  A. Neal,et al.  What can be inferred from bacterium–nanoparticle interactions about the potential consequences of environmental exposure to nanoparticles? , 2008, Ecotoxicology.

[38]  Menachem Elimelech,et al.  Single-walled carbon nanotubes exhibit strong antimicrobial activity. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[39]  H. Shapiro,et al.  Accurate flow cytometric membrane potential measurement in bacteria using diethyloxacarbocyanine and a ratiometric technique. , 1999, Cytometry.

[40]  P Stoodley,et al.  Survival strategies of infectious biofilms. , 2005, Trends in microbiology.