Stimulation of gonadotropin release by a non-GnRH peptide sequence of the GnRH precursor.

The human gonadotropin-releasing hormone (GnRH) precursor comprises the GnRH sequence followed by an extension of 59 amino acids. Basic amino acid residues in the carboxyl terminal extension may represent sites of processing to biologically active peptides. A synthetic peptide comprising the first 13 amino acids (H X Asp-Ala-Glu-Asn-Leu-Ile-Asp-Ser-Phe-Gln-Glu-Ile-Val X OH) of the 59-amino acid peptide was found to stimulate the release of gonadotropic hormones from human and baboon anterior pituitary cells in culture. The peptide did not affect thyrotropin or prolactin secretion. A GnRH antagonist did not inhibit gonadotropin stimulation by the peptide, and the peptide did not compete with GnRH for GnRH pituitary receptors, indicating that the action of the peptide is independent of the GnRH receptor. The GnRH precursor contains two distinct peptide sequences capable of stimulating gonadotropin release from human and baboon pituitary cells.

[1]  P. Seeburg,et al.  A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone , 1985, Nature.

[2]  P. Seeburg,et al.  Characterization of cDNA for precursor of human luteinizing hormone releasing hormone , 1984, Nature.

[3]  G. Hodgen,et al.  Medical hypophysectomy: I. Dose-response using a gonadotropin-releasing hormone antagonist. , 1984, Fertility and sterility.

[4]  R. Millar,et al.  Molecular Heterogeneity of Luteinizing Hormone-Releasing Hormone , 1984 .

[5]  S. Ojeda,et al.  Role of Peptides in the Control of Gonadotropin Secretion , 1984 .

[6]  J. Douglass,et al.  Polyprotein gene expression: generation of diversity of neuroendocrine peptides. , 1984, Annual review of biochemistry.

[7]  G. Fink,et al.  The human hypothalamic LHRH precursor is the same size as that in rat and mouse hypothalamus. , 1983, Biochemical and biophysical research communications.

[8]  R. Millar,et al.  Synthesis, luteinizing hormone-releasing activity, and receptor binding of chicken hypothalamic luteinizing hormone-releasing hormone. , 1983, Endocrinology.

[9]  R. Millar,et al.  Synthesis and biological activity of [D-Trp6] chicken luteinizing hormone-releasing hormone , 1983, Peptides.

[10]  G. Fink,et al.  A high molecular weight precursor of luteinizing hormone releasing hormone from rat hypothalamus. , 1983, Endocrinology.

[11]  C. Kordon,et al.  Occurrence of higher molecular forms of LHRH in fractionated extracts from rat hypothalamus, cortex and placenta , 1981, Molecular and Cellular Endocrinology.

[12]  R. Millar,et al.  Higher molecular weight immunoreactive species of luteinizing hormone releasing hormone: possible precursors of the hormone. , 1977, Biochemical and biophysical research communications.

[13]  A. Schally,et al.  Effect of active and passive immunization with luteinizing hormone-releasing hormone on serum luteinizing hormone and follicle-stimulating hormone levels and the ultrastructure of the pituitary gonadotrophs in castrated male rats. , 1976, Endocrinology.

[14]  A. Schally,et al.  Production of antiserum to LH-releasing hormone (LH-RH) associated with gonadal atrophy in rabbits: development of radioimmunoassays for LH-RH. , 1973, Endocrinology.

[15]  K. Folkers,et al.  Biosynthesis of the luteinizing hormone releasing hormone in mitochondrial preparations and by a possible pantetheine-template mechanism. , 1973, Biochemical and biophysical research communications.

[16]  C. Bowers,et al.  Biosynthesis in vitro of the luteinizing releasing hormone by hypothalamic tissue. , 1972, Biochemical and biophysical research communications.