Performance and microbial community dynamics in a two-phase anaerobic co-digestion system using cassava dregs and pig manure.

The two-phase anaerobic co-digestion of cassava dregs (CD) with pig manure (PM) was evaluated using four sequencing batch reactors (SBRs) and a continuously stirred tank reactor (CSTR). The effect of seven different PM to CD volatile solid ratios (10:0, 8:2, 6:4, 5:5, 4:6, 2:8 and 0:10) on the acidification phase was investigated. Results indicated the concentrations of soluble chemical oxygen demand, NH4-N and volatile fatty acids increased substantially at seven ratios. Co-acidification of PM and CD performed well. Methanogenic fermentation of the acidification products at seven ratios was steady in CSTR. The highest methane yield and VS removal of 0.352m(3)/kg VSadded and 68.5% were achieved at PM:CD (4:6). The microbial population in CSTR was analyzed using molecular methods. Findings revealed that bacteria such as Firmicutes and Bacteroidetes, archaea such as Methanobacteriales and Methanomicrobiales were advantageous populations. Co-digestion of PM and CD supported higher quantity and diversity of methanogens.

[1]  Xufeng Yuan,et al.  Microbial population dynamics and changes in main nutrients during the acidification process of pig manures. , 2011, Journal of environmental sciences.

[2]  S. Schmidt,et al.  Potential impact of process parameters upon the bacterial diversity in the mesophilic anaerobic digestion of beet silage. , 2011, Bioresource technology.

[3]  Irini Angelidaki,et al.  Influence of Environmental Conditions on Methanogenic Compositions in Anaerobic Biogas Reactors , 2022 .

[4]  Willy Verstraete,et al.  Methanosarcina: the rediscovered methanogen for heavy duty biomethanation. , 2012, Bioresource technology.

[5]  P. Funk,et al.  Anaerobic digestion of municipal solid waste and agricultural waste and the effect of co-digestion with dairy cow manure. , 2008, Bioresource technology.

[6]  Xiaofen Wang,et al.  Dynamic transition of microbial communities in response to acidification in fixed-bed anaerobic baffled reactors (FABR) of two different flow directions. , 2011, Bioresource technology.

[7]  Anna Schnürer,et al.  Effect of process temperature on bacterial and archaeal communities in two methanogenic bioreactors treating organic household waste. , 2007, FEMS microbiology ecology.

[8]  Seungyong Lee,et al.  Methanogenic community shift in anaerobic batch digesters treating swine wastewater. , 2010, Water research.

[9]  Heijo Scharff,et al.  Effect of pH and VFA on hydrolysis of organic solid waste , 2000 .

[10]  Pawinee Chaiprasert,et al.  Production of methane by co-digestion of cassava pulp with various concentrations of pig manure , 2010 .

[11]  I. Franke-Whittle,et al.  Archaeal community dynamics and abiotic characteristics in a mesophilic anaerobic co-digestion process treating fruit and vegetable processing waste sludge with chopped fresh artichoke waste. , 2013, Bioresource technology.

[12]  A. O'donnell,et al.  Microbial community dynamics in mesophilic anaerobic co-digestion of mixed waste. , 2011, Bioresource technology.

[13]  B. Demirel,et al.  The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review , 2008 .

[14]  P. Parameswaran,et al.  Anaerobic digestion and co-digestion processes of vegetable and fruit residues: process and microbial ecology. , 2011, Bioresource technology.

[15]  H. Goering,et al.  Forage fiber analyses (apparatus, reagents, prcedures, and some applications) , 1970 .

[16]  K. McMahon,et al.  Anaerobic codigestion of municipal solid waste and biosolids under various mixing conditions--II: Microbial population dynamics. , 2001, Water research.

[17]  Joan Mata-Álvarez,et al.  Anaerobic digestion of organic solid wastes. An overview of research achievements and perspectives , 2000 .

[18]  A. Uitterlinden,et al.  Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA , 1993, Applied and environmental microbiology.

[19]  Jaai Kim,et al.  Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction. , 2005, Biotechnology and bioengineering.

[20]  A. E. Greenberg,et al.  Standard Methods for the Examination of Water and Wastewater seventh edition , 2013 .

[21]  J. Zeikus,et al.  Biology, ecology, and biotechnological applications of anaerobic bacteria adapted to environmental stresses in temperature, pH, salinity, or substrates. , 1993, Microbiological reviews.

[22]  C.-L. Chen,et al.  Study of microbial community and biodegradation efficiency for single- and two-phase anaerobic co-digestion of brown water and food waste. , 2013, Bioresource technology.

[23]  S. Okabe,et al.  Functional bacterial and archaeal community structures of major trophic groups in a full-scale anaerobic sludge digester. , 2007, Water research.

[24]  S. Shin,et al.  Methanogenic population dynamics assessed by real-time quantitative PCR in sludge granule in upflow anaerobic sludge blanket treating swine wastewater. , 2010, Bioresource technology.

[25]  S. Hattori.  Syntrophic acetate-oxidizing microbes in methanogenic environments. , 2008, Microbes and environments.

[26]  R. Speece.  Anaerobic biotechnology for industrial wastewater treatment. , 1983, Environmental science & technology.

[27]  August Bonmatí,et al.  Air stripping of ammonia from pig slurry: characterisation and feasibility as a pre- or post-treatment to mesophilic anaerobic digestion. , 2003, Waste Management.

[28]  Moktar Hamdi,et al.  Bioreactor performance in anaerobic digestion of fruit and vegetable wastes , 2005 .

[29]  T. Lueders,et al.  Effects of Amendment with Ferrihydrite and Gypsum on the Structure and Activity of Methanogenic Populations in Rice Field Soil , 2002, Applied and Environmental Microbiology.

[30]  Hideki Harada,et al.  Diversity, Localization, and Physiological Properties of Filamentous Microbes Belonging to Chloroflexi Subphylum I in Mesophilic and Thermophilic Methanogenic Sludge Granules , 2005, Applied and Environmental Microbiology.