Rapid and Sensitive Detection of Bacteria Response to Antibiotics Using Nanoporous Membrane and Graphene Quantum Dot (GQDs)-Based Electrochemical Biosensors

The wide abuse of antibiotics has accelerated bacterial multiresistance, which means there is a need to develop tools for rapid detection and characterization of bacterial response to antibiotics in the management of infections. In the study, an electrochemical biosensor based on nanoporous alumina membrane and graphene quantum dots (GQDs) was developed for bacterial response to antibiotics detection. Anti-Salmonella antibody was conjugated with amino-modified GQDs by glutaraldehyde and immobilized on silanized nanoporous alumina membranes for Salmonella bacteria capture. The impedance signals across nanoporous membranes could monitor the capture of bacteria on nanoporous membranes as well as bacterial response to antibiotics. This nanoporous membrane and GQD-based electrochemical biosensor achieved rapid detection of bacterial response to antibiotics within 30 min, and the detection limit could reach the pM level. It was capable of investigating the response of bacteria exposed to antibiotics much more rapidly and conveniently than traditional tools. The capability of studying the dynamic effects of antibiotics on bacteria has potential applications in the field of monitoring disease therapy, detecting comprehensive food safety hazards and even life in hostile environment.

[1]  S. Levy,et al.  Food Animals and Antimicrobials: Impacts on Human Health , 2011, Clinical Microbiology Reviews.

[2]  S. Zeissig,et al.  Life at the beginning: perturbation of the microbiota by antibiotics in early life and its role in health and disease , 2014, Nature Immunology.

[3]  A. Chopra,et al.  Bio-nanomechanical Detection of Diabetic Marker HbA1c , 2012 .

[4]  S. Carda‐Broch,et al.  Analysis of selected veterinary antibiotics in fish by micellar liquid chromatography with fluorescence detection and validation in accordance with regulation 2002/657/EC , 2010 .

[5]  Z. Dai,et al.  Carbon nanomaterial-based electrochemical biosensors: an overview. , 2015, Nanoscale.

[6]  R. Timmons,et al.  Affinity mesh screen materials for selective extraction and analysis of antibiotics using transmission mode desorption electrospray ionization mass spectrometry. , 2013, Langmuir : the ACS journal of surfaces and colloids.

[7]  Abolfazl Barzegar,et al.  Riboswitches: From living biosensors to novel targets of antibiotics. , 2016, Gene.

[8]  Eduardo Gotuzzo,et al.  Rapid molecular detection of tuberculosis and rifampin resistance. , 2010, The New England journal of medicine.

[9]  A. Tomasz,et al.  The mechanism of the irreversible antimicrobial effects of penicillins: how the beta-lactam antibiotics kill and lyse bacteria. , 1979, Annual review of microbiology.

[10]  Michael C. McAlpine,et al.  Electrical detection of pathogenic bacteria via immobilized antimicrobial peptides , 2010, Proceedings of the National Academy of Sciences.

[11]  J. Heflin,et al.  Detection of methicillin-resistant staphylococci by biosensor assay consisting of nanoscale films on optical fiber long-period gratings. , 2015, Biosensors & bioelectronics.

[12]  A. Mak,et al.  A polyethylene glycol (PEG) microfluidic chip with nanostructures for bacteria rapid patterning and detection , 2009 .

[13]  H. Nau,et al.  Determination of persistent tetracycline residues in soil fertilized with liquid manure by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. , 2002, Analytical chemistry.

[14]  Jiewen Zhao,et al.  A magnetite/PMAA nanospheres-targeting SERS aptasensor for tetracycline sensing using mercapto molecules embedded core/shell nanoparticles for signal amplification. , 2017, Biosensors & bioelectronics.

[15]  P. Fey,et al.  Ceftriaxone-resistant salmonella infection acquired by a child from cattle. , 2000, The New England journal of medicine.

[16]  Yu Zhang,et al.  A Nanoporous Alumina Membrane Based Electrochemical Biosensor for Histamine Determination with Biofunctionalized Magnetic Nanoparticles Concentration and Signal Amplification , 2016, Sensors.

[17]  J. Collins,et al.  A Common Mechanism of Cellular Death Induced by Bactericidal Antibiotics , 2007, Cell.

[18]  Ron Jones,et al.  Does the use of antibiotics in food animals pose a risk to human health? A critical review of published data. , 2003, The Journal of antimicrobial chemotherapy.

[19]  Jing Lyu,et al.  Nanoparticle based fluorescence resonance energy transfer (FRET) for biosensing applications. , 2015, Journal of materials chemistry. B.

[20]  Alex Toftgaard Nielsen,et al.  Comparative study on aptamers as recognition elements for antibiotics in a label-free all-polymer biosensor. , 2013, Biosensors & bioelectronics.

[21]  Yu Zhang,et al.  A nanoporous membrane based impedance sensing platform for DNA sensing with gold nanoparticle amplification , 2014 .

[22]  Junping Wang,et al.  Development of an enzyme-linked immunosorbent assay for the detection of gentamycin residues in animal-derived foods , 2013 .

[23]  F. Portaels,et al.  Resazurin Microtiter Assay Plate: Simple and Inexpensive Method for Detection of Drug Resistance in Mycobacterium tuberculosis , 2002, Antimicrobial Agents and Chemotherapy.

[24]  A. Diacon,et al.  Time to detection of the growth of Mycobacterium tuberculosis in MGIT 960 for determining the early bactericidal activity of antituberculosis agents , 2010, European Journal of Clinical Microbiology & Infectious Diseases.

[25]  R. Fernández-Torres,et al.  Simultaneous determination of 11 antibiotics and their main metabolites from four different groups by reversed-phase high-performance liquid chromatography-diode array-fluorescence (HPLC-DAD-FLD) in human urine samples. , 2010, Talanta.

[26]  F. Borrull,et al.  Determination of antibiotic compounds in water by solid-phase extraction-high-performance liquid chromatography-(electrospray) mass spectrometry. , 2003, Journal of chromatography. A.

[27]  Ryan J. White,et al.  Enhancing the analytical performance of electrochemical RNA aptamer-based sensors for sensitive detection of aminoglycoside antibiotics. , 2014, Analytical chemistry.

[28]  W. Ye,et al.  Nanoporous membrane based impedance sensors to detect the enzymatic activity of botulinum neurotoxin A. , 2013, Journal of materials chemistry. B.

[29]  Jianhua Hao,et al.  Ultrasensitive Detection of Ebola Virus Oligonucleotide Based on Upconversion Nanoprobe/Nanoporous Membrane System. , 2016, ACS nano.

[30]  Suresh Neethirajan,et al.  Biosensors for the Detection of Antibiotics in Poultry Industry—A Review , 2014, Biosensors.

[31]  J. Mohapatra,et al.  Efficient synthesis of rice based graphene quantum dots and their fluorescent properties , 2016 .

[32]  H. Razmi,et al.  Graphene quantum dots as a new substrate for immobilization and direct electrochemistry of glucose oxidase: application to sensitive glucose determination. , 2013, Biosensors & bioelectronics.

[33]  Chunyan Zhang,et al.  Versatile immunosensor using a quantum dot coated silica nanosphere as a label for signal amplification. , 2010, Analytical chemistry.

[34]  Da Chen,et al.  Graphene-based materials in electrochemistry. , 2010, Chemical Society reviews.

[35]  Rashid Bashir,et al.  Electrical/electrochemical impedance for rapid detection of foodborne pathogenic bacteria. , 2008, Biotechnology advances.