Profiling of components of rhizoma et radix polygoni cuspidati by high-performance liquid chromatography with ultraviolet diode-array detector and ion trap/time-of-flight mass spectrometric detection

Introduction: Rhizoma et Radix Polygoni Cuspidati (Huzhang in Chinese, HZ) is a traditional medicinal plant in China. Many of the components of HZ have been proved to be bioactive while it is difficult to conduct a comprehensive chemical profiling of HZ as a consequence of the absence of efficient separation system and sensitive detective means. We developed a simple and effective method for comprehensive characterization of constituents in HZ. Objective: To develop a simple and effective method to characterize the components in HZ and provide useful information for subsequent metabolic studies of HZ. Materials and Methods: The components in HZ aqueous extract were characterized by using high performance liquid chromatography with UV diode-array detector (HPLC-DAD) and ion trap/time-of-flight mass spectrometric detection (HPLC-IT/TOF). Stilbenes, anthraquinones, gallates and tannins, naphthalenes and some other compounds were identified and confirmed by diagnostic fragment ions with accurate mass measurements, characteristic fragmentation pathways and relevant published literatures. Results: Among the 238 constituents detected in HZ, a total number of 74 constituents were identified unambiguously or tentatively, including 29 compounds reported for the first time in HZ. Conclusion: The identification and structure elucidation of these chemicals provided essential data for quality control and further in vivo metabolic studies of HZ. Key words: Polygonum cuspidatum, HPLC-DAD, HPLC-IT/TOF, qualitative analysis.

[1]  Lipeng Dong,et al.  Chemical Constituents of Polygonum cuspidatum , 2017, Chemistry of Natural Compounds.

[2]  Hong Wang,et al.  High-throughput and sensitive screening of compounds with deoxyribonucleic acid-binding activity by a high-performance liquid chromatography-tandem mass spectrometry-fluorescence detection technique using palmatine as a fluorescence probe. , 2014, Journal of chromatography. A.

[3]  Qing-Wen Zhang,et al.  A Review of the Pharmacological Effects of the Dried Root of Polygonum cuspidatum (Hu Zhang) and Its Constituents , 2013, Evidence-based complementary and alternative medicine : eCAM.

[4]  Hong Zhou,et al.  Botany, phytochemistry, pharmacology, and potential application of Polygonum cuspidatum Sieb.et Zucc.: a review. , 2013, Journal of ethnopharmacology.

[5]  Jing Zhang,et al.  Profiling of phenolic constituents in Polygonum multiflorum Thunb. by combination of ultra-high-pressure liquid chromatography with linear ion trap-Orbitrap mass spectrometry. , 2013, Journal of chromatography. A.

[6]  Huang Wei.  Extraction of Resveratrol from Polygonum cuspidatum , 2013 .

[7]  Shiuan-Pey Lin,et al.  A new herb-drug interaction of Polygonum cuspidatum, a resveratrol-rich nutraceutical, with carbamazepine in rats. , 2012, Toxicology and applied pharmacology.

[8]  Hai Wu,et al.  Liquid chromatography coupled with time-of-flight and ion trap mass spectrometry for qualitative analysis of herbal medicines , 2011, Journal of pharmaceutical analysis.

[9]  Zhang Guoqing.  Determination of multi-index components and quality control of Polygoni Cuspidati Rhizoma et Radix , 2011 .

[10]  Fengguo Xu,et al.  In vivo metabolism study of rhubarb decoction in rat using high-performance liquid chromatography with UV photodiode-array and mass-spectrometric detection: a strategy for systematic analysis of metabolites from traditional Chinese medicines in biological samples. , 2010, Journal of chromatography. A.

[11]  K. Hostettmann,et al.  Rapid separation of three glucosylated resveratrol analogues from the invasive plant Polygonum cuspidatum by high-speed countercurrent chromatography. , 2009, Journal of separation science.

[12]  Jing Dong,et al.  [Identification and determination of major constituents in Polygonum cuspidatum Sieb. et Zucc. by high performance liquid chromatography/electrospray ionization-ion trap-time-of-flight mass spectrometry]. , 2009, Se pu = Chinese journal of chromatography.

[13]  T. Hwang,et al.  Anthraquinones from Polygonum cuspidatum as tyrosinase inhibitors for dermal use , 2008, Phytotherapy research : PTR.

[14]  Z. Cai,et al.  Analysis of Rhizoma Polygoni Cuspidati by HPLC and HPLC-ESI/MS. , 2007, Phytochemical analysis : PCA.

[15]  H. Sheng.  Study on HPCE fingerprint of Rhizoma Polygoni Cuspidati , 2006 .

[16]  Y. Jun.  Extraction and GC/MS Analysis of Volatile Oil from Polygonum Cuspidatum , 2006 .

[17]  V. Gullo,et al.  Two new bacterial DNA primase inhibitors from the plant Polygonum cuspidatum. , 2004, Bioorganic & medicinal chemistry letters.

[18]  Young-Choon Lee,et al.  Inhibitory effects of Polygonum cuspidatum water extract (PCWE) and its component resveratrol [correction of rasveratrol] on acyl-coenzyme A-cholesterol acyltransferase activity for cholesteryl ester synthesis in HepG2 cells. , 2004, Vascular pharmacology.

[19]  Bai Dong-lu.  Studies on water-soluble constituents in rhizome of Polygonum cuspidatum , 2003 .

[20]  D. Bai,et al.  Constituents from Polygonum cuspidatum. , 2002, Chemical & pharmaceutical bulletin.

[21]  D. Bai,et al.  Stilbene glycoside sulfates from Polygonum cuspidatum , 2000 .

[22]  R. Geahlen,et al.  Kinase inhibitors from Polygonum cuspidatum. , 1993, Journal of natural products.

[23]  Y. Kimura,et al.  New Constitutents of Roots of Polygonum cuspidatum. , 1983, Planta medica.