Addressable electrode array device with IDA electrodes for high-throughput detection.

An electrochemical device is proposed for high-throughput electrochemical detection that consists of 32 row and 32 column electrodes on a single glass substrate. The row and column electrodes are connected to interdigitated array (IDA) electrodes to form 1024 (32 × 32) addressable sensor points in the device. Electrochemical responses from each of the 1024 sensors were successfully acquired on the device within 1 min using redox cycling at individual IDA electrodes, which ensures application of the device to comprehensive, high-throughput electrochemical detection for enzyme-linked immunosorbent assay (ELISA), reporter gene assay for monitoring gene expressions, and DNA analysis.

[1]  K. Ino,et al.  Electrochemical topography of a cell monolayer with an addressable microelectrode array. , 2010, Chemical communications.

[2]  K. Djupsund,et al.  Flexible polyimide microelectrode array for in vivo recordings and current source density analysis. , 2007, Biosensors & bioelectronics.

[3]  W. Heineman,et al.  Small-volume voltammetric detection of 4-aminophenol with interdigitated array electrodes and its application to electrochemical enzyme immunoassay. , 1993, Analytical chemistry.

[4]  M. Koudelka-Hep,et al.  Individually addressable gel-integrated voltammetric microelectrode array for high-resolution measurement of concentration profiles at interfaces. , 2001, Analytical chemistry.

[5]  R. Hintsche,et al.  Automated detection and quantitation of bacterial RNA by using electrical microarrays. , 2006, Analytical chemistry.

[6]  Hitoshi Shiku,et al.  An addressable microelectrode array for electrochemical detection. , 2008, Analytical chemistry.

[7]  K. Ino,et al.  Electrochemical gene-function analysis for single cells with addressable microelectrode/microwell arrays. , 2009, Angewandte Chemie.

[8]  A L Ghindilis,et al.  Immunoassays based on electrochemical detection using microelectrode arrays. , 2004, Biosensors & bioelectronics.

[9]  K. Ino,et al.  Addressable electrochemiluminescence detection system based on redox-cycling of Ru(bpy)(3)(2+). , 2010, Chemical communications.

[10]  Song Zhang,et al.  Integration of electrochemistry in micro-total analysis systems for biochemical assays: recent developments. , 2009, Talanta.