Efficient production of l-lactic acid by newly isolated thermophilic Bacillus coagulans WCP10-4 with high glucose tolerance

A thermophilic Bacillus coagulans WCP10-4 with tolerance to high concentration of glucose was isolated from soil and used to produce optically pure l-lactic acid from glucose and starch. In batch fermentation at pH 6.0, 240 g/L of glucose was completely consumed giving 210 g/L of l-lactic acid with a yield of 95 % and a productivity of 3.5 g/L/h. In simultaneous saccharification and fermentation at 50 °C without sterilizing the medium, 200 g/L of corn starch was completely consumed producing 202.0 g/L of l-lactic acid. To the best of our knowledge, this strain shows the highest osmotic tolerance to glucose among the strains ever reported for lactic acid production. This is the first report of simultaneous saccharification and fermentation of starch for lactic acid production under a non-sterilized condition.

[1]  Cuiqing Ma,et al.  Efficient production of L-lactic acid from cassava powder by Lactobacillus rhamnosus. , 2010, Bioresource technology.

[2]  M. Rebroš,et al.  High Temperature Lactic Acid Production by Bacillus coagulans Immobilized in LentiKats , 2005, Biotechnology Letters.

[3]  K. Shanmugam,et al.  Isolation, characterization and evolution of a new thermophilic Bacillus licheniformis for lactic acid production in mineral salts medium. , 2011, Bioresource technology.

[4]  K. Shanmugam,et al.  l(+)-Lactic acid production from non-food carbohydrates by thermotolerant Bacillus coagulans , 2011, Journal of Industrial Microbiology & Biotechnology.

[5]  K Madhavan Nampoothiri,et al.  Direct lactic acid fermentation: focus on simultaneous saccharification and lactic acid production. , 2009, Biotechnology advances.

[6]  I. Suitso,et al.  l(+)-Lactic acid producer Bacillus coagulans SIM-7 DSM 14043 and its comparison with Lactobacillus delbrueckii ssp. lactis DSM 20073 , 2006 .

[7]  Y. Tashiro,et al.  Continuous d-lactic acid production by a novelthermotolerant Lactobacillus delbrueckii subsp. lactis QU 41 , 2011, Applied Microbiology and Biotechnology.

[8]  Tianwei Tan,et al.  L‐Lactic acid production by Lactobacillus casei fermentation with corn steep liquor‐supplemented acid‐hydrolysate of soybean meal , 2006, Biotechnology journal.

[9]  K. Shanmugam,et al.  Isolation and Characterization of Acid-Tolerant, Thermophilic Bacteria for Effective Fermentation of Biomass-Derived Sugars to Lactic Acid , 2006, Applied and Environmental Microbiology.

[10]  K. Turksen,et al.  Isolation and characterization , 2006 .

[11]  Wei-guo Zhang,et al.  Improvement of l-lactic acid production by osmotic-tolerant mutant of Lactobacillus casei at high temperature , 2010, Applied Microbiology and Biotechnology.

[12]  Gi-wook Choi,et al.  A novel lactic acid bacterium for the production of high purity L-lactic acid, Lactobacillus paracasei subsp. paracasei CHB2121. , 2012, Journal of bioscience and bioengineering.

[13]  Ping Xu,et al.  Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 , 2009, PloS one.

[14]  T. Tan,et al.  Improvement of l-Lactic Acid Production under Glucose Feedback Controlled Culture by Lactobacillus rhamnosus , 2010, Applied biochemistry and biotechnology.

[15]  Akihiko Kondo,et al.  Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limits , 2009, Applied Microbiology and Biotechnology.

[16]  Pradip K. Roychoudhury,et al.  L (+) lactic acid fermentation and its product polymerization , 2004 .

[17]  E. Vijay Kumar,et al.  Amylolytic bacterial lactic acid fermentation - a review. , 2008, Biotechnology advances.

[18]  Rathin Datta,et al.  Lactic acid: recent advances in products, processes and technologies — a review , 2006 .

[19]  K. Sakai,et al.  Thermotolerant Bacillus licheniformis TY7 produces optically active l-lactic acid from kitchen refuse under open condition. , 2006, Journal of bioscience and bioengineering.

[20]  Xin-Gang Li,et al.  Fed-batch fermentation of Lactobacillus lactis for hyper-production of l-lactic acid , 2003, Biotechnology Letters.

[21]  A. Heiningen,et al.  Production of lactic acid from hemicellulose extracts by Bacillus coagulans MXL-9 , 2010, Journal of Industrial Microbiology & Biotechnology.

[22]  Yanhe Ma,et al.  Efficient production of L-lactic acid with high optical purity by alkaliphilic Bacillus sp. WL-S20. , 2012, Bioresource technology.

[23]  Cuiqing Ma,et al.  Efficient production of L-lactic acid from corncob molasses, a waste by-product in xylitol production, by a newly isolated xylose utilizing Bacillus sp. strain. , 2010, Bioresource technology.

[24]  Eva R. Kashket,et al.  Uncoupling by Acetic Acid Limits Growth of and Acetogenesis by Clostridium thermoaceticum , 1984, Applied and environmental microbiology.