Functionalized-graphene modified graphite electrode for the selective determination of dopamine in presence of uric acid and ascorbic acid.

Graphene is chemically synthesized by solvothermal reduction of colloidal dispersions of graphite oxide. Graphite electrode is modified with functionalized-graphene for electrochemical applications. Electrochemical characterization of functionalized-graphene modified graphite electrode (FGGE) is carried out by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The behavior of FGGE towards ascorbic acid (AA), dopamine (DA) and uric acid (UA) has been investigated by CV, differential pulse voltammetry (DPV) and chronoamperommetry (CA). The FGGE showed excellent catalytic activity towards electrochemical oxidation of AA, DA and UA compared to that of the bare graphite electrode. The electrochemical oxidation signals of AA, DA and UA are well separated into three distinct peaks with peak potential separation of 193mv, 172mv and 264mV between AA-DA, DA-UA and AA-UA respectively in CV studies and the corresponding peak potential separations in DPV mode are 204mv, 141mv and 345mv. The FGGE is successfully used for the simultaneous detection of AA, DA and UA in their ternary mixture and DA in serum and pharmaceutical samples. The excellent electrocatalytic behavior of FGGE may lead to new applications in electrochemical analysis.

[1]  K. Müllen,et al.  Transparent, conductive graphene electrodes for dye-sensitized solar cells. , 2008, Nano letters.

[2]  Joseph Wang.  Carbon‐Nanotube Based Electrochemical Biosensors: A Review , 2005 .

[3]  Bin Fang,et al.  Fabrication of Fe3O4 Nanoparticles Modified Electrode and Its Application for Voltammetric Sensing of Dopamine , 2005 .

[4]  Xiangqin Lin,et al.  RNA Modified Electrodes for Simultaneous Determination of Dopamine and Uric Acid in the Presence of High Amounts of Ascorbic Acid , 2006 .

[5]  Yogeswaran Umasankar,et al.  Nanocomposite of functionalized multiwall carbon nanotubes with nafion, nano platinum, and nano gold biosensing film for simultaneous determination of ascorbic acid, epinephrine, and uric acid. , 2007, Analytical biochemistry.

[6]  Chen-Zhong Li,et al.  Probing the Electrochemical Properties of Graphene Nanosheets for Biosensing Applications , 2009 .

[7]  Yongxin Li,et al.  Fabrication of layer-by-layer modified multilayer films containing choline and gold nanoparticles and its sensing application for electrochemical determination of dopamine and uric acid. , 2007, Talanta.

[8]  Y. Tsai,et al.  Graphene modified basal and edge plane pyrolytic graphite electrodes for electrocatalytic oxidation of hydrogen peroxide and β-nicotinamide adenine dinucleotide , 2009 .

[9]  G. Suresh,et al.  Detection of Uric Acid in the Presence of Dopamine and High Concentration of Ascorbic Acid Using PDDA Modified Graphite Electrode , 2009 .

[10]  S. F. D’souza,et al.  Simultaneous determination of ascorbic acid, dopamine and uric acid using polystyrene sulfonate wrapped multiwalled carbon nanotubes bound to graphite electrode through layer-by-layer technique , 2010 .

[11]  Jun Liu,et al.  Glucose oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. , 2009, Biosensors & bioelectronics.

[12]  Huafeng Yang,et al.  Graphene/AuNPs/chitosan nanocomposites film for glucose biosensing. , 2010, Biosensors & bioelectronics.

[13]  S. Dong,et al.  Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide. , 2009, Analytical chemistry.

[14]  Ying Wang,et al.  Application of graphene-modified electrode for selective detection of dopamine , 2009 .

[15]  Huafeng Yang,et al.  Direct electrochemistry of glucose oxidase and biosensing for glucose based on graphene. , 2009, Analytical chemistry.

[16]  Guangfeng Wang,et al.  Simultaneous determination of dopamine, uric acid and ascorbic acid with LaFeO3 nanoparticles modified electrode , 2009 .

[17]  R M Westervelt,et al.  Graphene Nanoelectronics , 2008, Science.

[18]  M. Rajamathi,et al.  CHEMICALLY MODIFIED GRAPHENE SHEETS PRODUCED BY THE SOLVOTHERMAL REDUCTION OF COLLOIDAL DISPERSIONS OF GRAPHITE OXIDE , 2008 .

[19]  Jun Liu,et al.  Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film. , 2009, Talanta.

[20]  Mianqi Xue,et al.  Processing of graphene for electrochemical application: noncovalently functionalize graphene sheets with water-soluble electroactive methylene green. , 2009, Langmuir : the ACS journal of surfaces and colloids.

[21]  A. Govindaraj,et al.  Graphene-based electrochemical supercapacitors , 2008 .

[22]  Michel Jouvet,et al.  In vivo electrochemical detection of catechols in the neostriatum of anaesthetized rats: dopamine or DOPAC? , 1980, Nature.

[23]  Liping Lu,et al.  DNA/Poly(p-aminobenzensulfonic acid) composite bi-layer modified glassy carbon electrode for determination of dopamine and uric acid under coexistence of ascorbic acid. , 2007, Bioelectrochemistry.

[24]  T. Ohsaka,et al.  Gold nanoparticle arrays for the voltammetric sensing of dopamine , 2003 .

[25]  Q. Jiang,et al.  Enhancement of CO detection in Al doped graphene , 2008, 0806.3172.

[26]  Priscilla Kailian Ang,et al.  Solution-gated epitaxial graphene as pH sensor. , 2008, Journal of the American Chemical Society.

[27]  G. Suresh,et al.  Selective determination of dopamine using unmodified, exfoliated graphite electrodes , 2004 .

[28]  Itaru Honma,et al.  Biosensing Properties of TitanateNanotube Films: Selective Detection of Dopamine in the Presence of Ascorbate and Uric Acid , 2006 .