Formaldehyde promotes and inhibits the proliferation of cultured tumour and endothelial cells

Formaldehyde was applied in various doses (0.1–10.0 mm) to HT‐29 human colon carcinoma and HUV‐EC‐C human endothelial cell cultures. Cell number, apoptotic and mitotic index as well as proportion of cells in S‐phase was investigated by morphological methods and flow cytometry. Ten mM of formaldehyde caused high degree of cell damage and practically eradicated the cell cultures. One mM of formaldehyde enhanced apoptosis and reduced mitosis in both types of cell cultures, in a moderate manner. The low dose (0.1 mm) enhanced cell proliferation and decreased apoptotic activity of the cultured cells, the tumour cells appeared to be more sensitive. The possible role of this dose‐dependent effect of formaldehyde in various pathological conditions, such as carcinogenesis and atherogenesis is discussed with emphasis on the eventual interaction between formaldehyde and hydrogen peroxide.

[1]  P. Morselli,et al.  The enzymatic systems involved in the mammalian metabolism of methylamine. , 1985, General pharmacology.

[2]  T. Starr,et al.  Formaldehyde toxicity--new understanding. , 1990, Critical reviews in toxicology.

[3]  Z. Darżynkiewicz,et al.  A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. , 1994, Analytical biochemistry.

[4]  K. Morgan,et al.  Chemically-induced nasal carcinogenesis and epithelial cell proliferation: a brief review. , 1997, Mutation research.

[5]  R. Gáborjányi,et al.  Possibility of formation of excited formaldehyde and singlet oxygen in biotic and abiotic stress situations. , 1994, Acta biologica Hungarica.

[6]  H. Cedar,et al.  Dynamics of DNA methylation during development , 1993, BioEssays : news and reviews in molecular, cellular and developmental biology.

[7]  E. Tyihák,et al.  Formation of formaldehyde from S‐adenosyl‐L[methyl‐3H]methionme during enzymic transmethylation of histamine , 1986, FEBS letters.

[8]  A. Wyllie,et al.  Cell death: the significance of apoptosis. , 1980, International review of cytology.

[9]  L. Oreland,et al.  Plasma Semicarbazide-Sensitive Amine Oxidase in Stroke , 1999, European Neurology.

[10]  Kawata Sumio,et al.  Hepatic microsomal cytochrome p-450-dependent N-demethylation of methylguanidine. , 1983 .

[11]  B. Szende,et al.  Formaldehyde Cycle and the Phases of Stress Syndrome , 1998 .

[12]  J. Ribak,et al.  DNA--protein crosslinks, a biomarker of exposure to formaldehyde--in vitro and in vivo studies. , 1996, Carcinogenesis.

[13]  G. Ansari,et al.  Methylamine metabolism to formaldehyde by vascular semicarbazide-sensitive amine oxidase. , 1992, Toxicology.

[14]  B. Szende,et al.  Formaldehyde generators and capturers as influencing factors of mitotic and apoptotic processes. , 1998, Acta biologica Hungarica.

[15]  B. Szende,et al.  Formaldehyde Cycle and the Natural Formaldehyde Generators and Capturers , 1998, Acta biologica Hungarica.

[16]  S. Lord-Fontaine,et al.  Enhancement of cytotoxicity of hydrogen peroxide by hyperthermia in chinese hamster ovary cells: role of antioxidant defenses. , 1999, Archives of biochemistry and biophysics.

[17]  B. Szende,et al.  Apoptosis as a possible way of destruction of lymphoblasts after glucocorticoid treatment of children with acute lymphoblastic leukemia. , 1994, Pediatric hematology and oncology.

[18]  R. Mihalik,et al.  Modulation of drug-induced apoptosis in a human B-lymphoma cell line (HT58). , 1995, Immunology letters.

[19]  I. Fridovich,et al.  Superoxide radical and superoxide dismutases. , 1995, Annual review of biochemistry.

[20]  J. Pipek,et al.  Formation of excited formaldehyde in model reactions simulating real biological systems , 1988 .

[21]  R. Sofia,et al.  The formaldehyde-donating activity of N5,N10-methylene tetrahydrofolic acid in xenobiotic biotransformation. , 1984, Xenobiotica; the fate of foreign compounds in biological systems.