Plant gamma-thionins: novel insights on the mechanism of action of a multi-functional class of defense proteins.

[1]  J. Winderickx,et al.  SKN1, a novel plant defensin‐sensitivity gene in Saccharomyces cerevisiae, is implicated in sphingolipid biosynthesis , 2005, FEBS letters.

[2]  M. Anderson,et al.  Defensins--components of the innate immune system in plants. , 2005, Current protein & peptide science.

[3]  Huei-Mei Chen,et al.  Cloning and characterization of a plant defensin VaD1 from azuki bean. , 2005, Journal of agricultural and food chemistry.

[4]  D. Andreu,et al.  Structural Dissection of a Highly Knotted Peptide Reveals Minimal Motif with Antimicrobial Activity* , 2005, Journal of Biological Chemistry.

[5]  M. Fujimura,et al.  Amino Acid Sequence and Antimicrobial Activity of Chitin-Binding Peptides, Pp-AMP 1 and Pp-AMP 2, from Japanese Bamboo Shoots (Phyllostachys pubescens) , 2005, Bioscience, biotechnology, and biochemistry.

[6]  T. Smith,et al.  Differential Antifungal and Calcium Channel-Blocking Activity among Structurally Related Plant Defensins1[w] , 2004, Plant Physiology.

[7]  J. Oard,et al.  Characterization of antimicrobial peptides against a US strain of the rice pathogen Rhizoctonia solani , 2004, Journal of Applied Microbiology.

[8]  B. Thomma,et al.  Defensins from Insects and Plants Interact with Fungal Glucosylceramides* , 2004, Journal of Biological Chemistry.

[9]  M. Fujimura,et al.  Purification, Characterization, and Sequencing of Novel Antimicrobial Peptides, Tu-AMP 1 and Tu-AMP 2, from Bulbs of Tulip (Tulipa gesneriana L.) , 2004, Bioscience, biotechnology, and biochemistry.

[10]  K. Apel,et al.  Organ-specific expression of highly divergent thionin variants that are distinct from the seed-specific crambin in the crucifer Crambe abyssinica , 1994, Molecular and General Genetics MGG.

[11]  W. Stiekema,et al.  Thionins: properties, possible biological roles and mechanisms of action , 1994, Plant Molecular Biology.

[12]  A. Cheung,et al.  A flower-specific cDNA encoding a novel thionin in tobacco , 1992, Molecular and General Genetics MGG.

[13]  K. Apel,et al.  A comparison of leaf thionin sequences of barley cultivars and wild barley species , 1992, Molecular and General Genetics MGG.

[14]  W. Gong,et al.  cDNA cloning, functional expression and antifungal activities of a dimeric plant defensin SPE10 from Pachyrrhizus erosus seeds , 2004, Plant Molecular Biology.

[15]  H. Molinari,et al.  NMR solution structure of viscotoxin C1 from Viscum album species Coloratum ohwi: toward a structure-function analysis of viscotoxins. , 2003, Biochemistry.

[16]  B. Cammue,et al.  Interactions of antifungal plant defensins with fungal membrane components , 2003, Peptides.

[17]  S. Levery,et al.  Isolation and characterization of Neurospora crassa mutants resistant to antifungal plant defensins. , 2003, Fungal genetics and biology : FG & B.

[18]  L. Vernon,et al.  Stimulation of prothrombinase activity by the nonapeptide Thr-Trp-Ala-Arg-Asn-Ser-Tyr-Asn-Val, a segment of a plant thionin , 2003, Peptides.

[19]  C. Renner,et al.  Structural characterization of hellethionins from Helleborus purpurascens. , 2003, Biochemistry.

[20]  B. Thomma,et al.  Mode of action of plant defensins suggests therapeutic potential. , 2003, Current drug targets. Infectious disorders.

[21]  David Andreu,et al.  Synthetic and structural studies on Pyrularia pubera thionin: a single‐residue mutation enhances activity against Gram‐negative bacteria , 2003, FEBS letters.

[22]  D. Craik,et al.  The three-dimensional solution structure of NaD1, a new floral defensin from Nicotiana alata and its application to a homology model of the crop defense protein alfAFP. , 2003, Journal of molecular biology.

[23]  M. Fujimura,et al.  Purification, Characterization, and Sequencing of a Novel Type of Antimicrobial Peptides, Fa-AMP1 and Fa-AMP2, from Seeds of Buckwheat (Fagopyrum esculentum Moench.) , 2003, Bioscience, biotechnology, and biochemistry.

[24]  L. Bohlin,et al.  Small, novel proteins from the mistletoe Phoradendron tomentosum exhibit highly selective cytotoxicity to human breast cancer cells , 2003, Cellular and Molecular Life Sciences CMLS.

[25]  S. Tsuda,et al.  A novel plant defensin-like gene of winter wheat is specifically induced during cold acclimation. , 2002, Biochemical and biophysical research communications.

[26]  L. Bohlin,et al.  Ligatoxin B, a new cytotoxic protein with a novel helix-turn-helix DNA-binding domain from the mistletoe Phoradendron liga. , 2002, The Biochemical journal.

[27]  D. Rigden,et al.  Inhibition of trypsin by cowpea thionin: Characterization, molecular modeling, and docking , 2002, Proteins.

[28]  T. Sasaki,et al.  Enhanced resistance to seed-transmitted bacterial diseases in transgenic rice plants overproducing an oat cell-wall-bound thionin. , 2002, Molecular plant-microbe interactions : MPMI.

[29]  H. Vogel,et al.  The Solution Structures of the Human β-Defensins Lead to a Better Understanding of the Potent Bactericidal Activity of HBD3 against Staphylococcus aureus * , 2002, The Journal of Biological Chemistry.

[30]  C. W. Hilbers,et al.  Improving the quality of protein structures derived by NMR spectroscopy** , 2002, Journal of biomolecular NMR.

[31]  M. Berrocal-Lobo,et al.  Snakin-2, an Antimicrobial Peptide from Potato Whose Gene Is Locally Induced by Wounding and Responds to Pathogen Infection1 , 2002, Plant Physiology.

[32]  M. Almeida,et al.  Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensins, identical backbone with different mechanisms of action. , 2002, Journal of molecular biology.

[33]  D. Rigden,et al.  Plant α‐amylase inhibitors and their interaction with insect α‐amylases , 2002 .

[34]  The expression of tgas118, encoding a defensin in Lycopersicon esculentum, is regulated by gibberellin. , 2001, Journal of experimental botany.

[35]  L. Sijtsma,et al.  Synthetic peptides derived from the beta2-beta3 loop of Raphanus sativus antifungal protein 2 that mimic the active site. , 2001, The journal of peptide research : official journal of the American Peptide Society.

[36]  W. Peng,et al.  An Arabidopsis mutant cex1 exhibits constant accumulation of jasmonate‐regulated AtVSP, Thi2.1 and PDF1.2 , 2001, FEBS letters.

[37]  D. Shah,et al.  Fungal pathogen protection in potato by expression of a plant defensin peptide , 2000, Nature Biotechnology.

[38]  Hughes,et al.  Defense proteins from seed of Cassia fistula include a lipid transfer protein homologue and a protease inhibitory plant defensin. , 2000, Plant science : an international journal of experimental plant biology.

[39]  M. Almeida,et al.  Characterization of two novel defense peptides from pea (Pisum sativum) seeds. , 2000, Archives of biochemistry and biophysics.

[40]  V Lamzin,et al.  Accurate protein crystallography at ultra-high resolution: valence electron distribution in crambin. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[41]  P. Gage,et al.  The Cytotoxic Plant Protein, β-Purothionin, Forms Ion Channels in Lipid Membranes* , 2000, The Journal of Biological Chemistry.

[42]  M. Ohnishi-Kameyama,et al.  Binding of Barley and Wheat α-Thionins to Polysaccharides , 2000 .

[43]  M. M. Cowan.  Plant Products as Antimicrobial Agents , 1999, Clinical Microbiology Reviews.

[44]  G. Schaller,et al.  Accidental cell death and generation of reactive oxygen intermediates in human lymphocytes induced by thionins from Viscum album L. , 1999, European journal of biochemistry.

[45]  S. M. de Freitas,et al.  Thermodynamics of the Binding of Chymotrypsin with the Black-eyed Pea Trypsin and Chymotrypsin Inhibitor (BTCI) , 1999, Journal of protein chemistry.

[46]  T. Walsh,et al.  Antifungal Peptides: Novel Therapeutic Compounds against Emerging Pathogens , 1999, Antimicrobial Agents and Chemotherapy.

[47]  C. Kushmerick,et al.  Functional and structural features of γ‐zeathionins, a new class of sodium channel blockers , 1998 .

[48]  O. Froy,et al.  Membrane potential modulators: a thread of scarlet from plants to humans , 1998, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[49]  G. Schaller,et al.  Generation of reactive oxygen intermediates (ROI) by the thionins from Viscum album L. , 1998, Anticancer research.

[50]  C. Wasternack,et al.  Wounding and chemicals induce expression of the Arabidopsis thaliana gene Thi2.1, encoding a fungal defense thionin, via the octadecanoid pathway , 1998, FEBS letters.

[51]  M. Moreno,et al.  Novel defensin subfamily from spinach (Spinacia oleracea) , 1998, FEBS letters.

[52]  J M Thornton,et al.  1H NMR structure of an antifungal γ‐thionin protein SIα1: Similarity to scorpion toxins , 1998, Proteins.

[53]  W. Vranken,et al.  Determination of the three-dimensional solution structure of Raphanus sativus antifungal protein 1 by 1H NMR. , 1998, Journal of molecular biology.

[54]  C. Wasternack,et al.  Systemic and local induction of an Arabidopsis thionin gene by wounding and pathogens. , 1998, The Plant journal : for cell and molecular biology.

[55]  A. Molina,et al.  Plant defense peptides. , 1998, Biopolymers.

[56]  E. Titarenko,et al.  Mutants of Ralstonia (Pseudomonas) solanacearum sensitive to antimicrobial peptides are altered in their lipopolysaccharide structure and are avirulent in tobacco , 1997, Journal of bacteriology.

[57]  A. Scaloni,et al.  Amino Acid Sequence, S-S Bridge Arrangement and Distribution in Plant Tissues of Thionins from Viscum album , 1997, Biological chemistry.

[58]  F. Goñi,et al.  Differential effects of five types of antipathogenic plant peptides on model membranes , 1997, FEBS letters.

[59]  M. Teeter,et al.  Crystal Structure of Ser-22/Ile-25 Form Crambin Confirms Solvent, Side Chain Substate Correlations* , 1997, The Journal of Biological Chemistry.

[60]  P. Epple,et al.  Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum. , 1997, The Plant cell.

[61]  N. Guex,et al.  SWISS‐MODEL and the Swiss‐Pdb Viewer: An environment for comparative protein modeling , 1997, Electrophoresis.

[62]  V. Stojanoff,et al.  Expression, purification and characterization of recombinant crambin. , 1996, Protein engineering.

[63]  M. Calero,et al.  Primary structure of omega-hordothionin, a member of a novel family of thionins from barley endosperm, and its inhibition of protein synthesis in eukaryotic and prokaryotic cell-free systems. , 1996, European journal of biochemistry.

[64]  C. Brownlee,et al.  Fungal Membrane Responses Induced by Plant Defensins and Thionins* , 1996, The Journal of Biological Chemistry.

[65]  T. Moon,et al.  Determination of the three-dimensional structure of hordothionin-α by nuclear magnetic resonance , 1996 .

[66]  E. Méndez,et al.  1H‐nmr studies on the structure of a new thionin from barley endosperm , 1995, Biopolymers.

[67]  P. Epple,et al.  An Arabidopsis thaliana Thionin Gene Is Inducible via a Signal Transduction Pathway Different from That for Pathogenesis-Related Proteins , 1995, Plant physiology.

[68]  E. Titarenko,et al.  Selective disulphide linkage of plant thionins with other proteins , 1995, FEBS letters.

[69]  N V Raikhel,et al.  Small cysteine-rich antifungal proteins from radish: their role in host defense. , 1995, The Plant cell.

[70]  G. Marino,et al.  Amino acid sequence and disulphide-bridge pattern of three γ-thionins from Sorghum bicolor , 1995 .

[71]  P. Carbonero,et al.  cDNA Cloning and Nucleotide Sequences of [alpha]1 and [alpha]2 Thionins from Hexaploid Wheat Endosperm , 1994, Plant physiology.

[72]  A. Segura,et al.  Pseudothionin-St1, a potato peptide active against potato pathogens. , 1994, European journal of biochemistry.

[73]  Correlated disorder of the pure Pro22/Leu25 form of crambin at 150 K refined to 1.05-A resolution. , 1994, The Journal of biological chemistry.

[74]  Franky R. G. Terras,et al.  Synergistic Enhancement of the Antifungal Activity of Wheat and Barley Thionins by Radish and Oilseed Rape 2S Albumins and by Barley Trypsin Inhibitors , 1993, Plant physiology.

[75]  A. Molina,et al.  Expression of the alpha-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens. , 1993, The Plant journal : for cell and molecular biology.

[76]  E. Méndez,et al.  Solution structure of gamma 1-H and gamma 1-P thionins from barley and wheat endosperm determined by 1H-NMR: a structural motif common to toxic arthropod proteins. , 1993, Biochemistry.

[77]  A. Castagnaro,et al.  Extreme divergence of a novel wheat thionin generated by a mutational burst specifically affecting the mature protein domain of the precursor. , 1992, Journal of molecular biology.

[78]  I. Díaz,et al.  Effects of thionins on β‐glucuronidase in vitro and in plant protoplasts , 1992 .

[79]  W. K. Roberts,et al.  A new family of plant antifungal proteins. , 1991, Molecular plant-microbe interactions : MPMI.

[80]  C. Bloch,et al.  A new family of small (5 kDa) protein inhibitors of insect α‐amylases from seeds or sorghum (Sorghum bicolor (L) Moench) have sequence homologies with wheat γ‐purothionins , 1991 .

[81]  C. de Haro,et al.  Primary structure and inhibition of protein synthesis in eukaryotic cell-free system of a novel thionin, gamma-hordothionin, from barley endosperm. , 1990, European journal of biochemistry.

[82]  E. Méndez,et al.  γ‐Purothionins: amino acid sequence of two polypeptides of a new family of thionins from wheat endosperm , 1990, FEBS letters.

[83]  M. Whitlow,et al.  Crystal structure of a protein‐toxin α1‐purothionin at 2.5Å and a comparison with predicted models , 1990, Proteins.

[84]  H. Nikaido.  Outer membrane barrier as a mechanism of antimicrobial resistance , 1989, Antimicrobial Agents and Chemotherapy.

[85]  L. Vernon,et al.  Hemolytic activity of thionin from Pyrularia pubera nuts and snake venom toxins of Naja naja species: Pyrularia thionin and snake venom cardiotoxin compete for the same membrane site. , 1989, Toxicon : official journal of the International Society on Toxinology.

[86]  W. Gray,et al.  A toxic thionin from Pyrularia pubera: purification, properties, and amino acid sequence. , 1985, Archives of biochemistry and biophysics.

[87]  M. Teeter,et al.  Primary structure of the hydrophobic plant protein crambin. , 1981, Biochemistry.

[88]  J Deisenhofer,et al.  Structure of the complex formed by bovine trypsin and bovine pancreatic trypsin inhibitor. II. Crystallographic refinement at 1.9 A resolution. , 1974, Journal of molecular biology.

[89]  R. Huber,et al.  Structure of the complex formed by bovine trypsin and bovine pancreatic trypsin inhibitor. Crystal structure determination and stereochemistry of the contact region. , 1973, Journal of molecular biology.

[90]  B. D. Davis.  Principles of microbiology and immunology. , 1968 .