Biodefense-Driven Murine Model of Pneumonic Melioidosis

ABSTRACT A whole-body mouse model of pneumonic melioidosis was established for future evaluation of biodefense vaccine candidates. The aerosol 50% lethal doses of Burkholderia pseudomallei strain 1026b for BALB/c and C57BL/6 mice and the times to death, dissemination in organs, and tissue loads after exposure of the mice to low- and high-dose aerosols are reported. In addition, rpsL mutant backgrounds were attenuated in this acute model of disease.

[1]  Y. Gan,et al.  Model of Differential Susceptibility to Mucosal Burkholderia pseudomallei Infection , 2002, Infection and Immunity.

[2]  M. Bray,et al.  Treatment of aerosolized cowpox virus infection in mice with aerosolized cidofovir , 2001, Antiviral research.

[3]  N. Ketheesan,et al.  Induction of multiple chemokine and colony‐stimulating factor genes in experimental Burkholderia pseudomallei infection , 2001, Immunology and cell biology.

[4]  D. Waag,et al.  In Vitro Antibiotic Susceptibilities ofBurkholderia mallei (Causative Agent of Glanders) Determined by Broth Microdilution and E-Test , 2001, Antimicrobial Agents and Chemotherapy.

[5]  A. White,et al.  Tropical Infectious Diseases: Principles, Pathogens, and Practice , 2000, Annals of Internal Medicine.

[6]  D. Waag,et al.  The Hamster Model of Intraperitoneal Burkholderia mallei (Glanders) , 1999, Veterinary pathology.

[7]  G. Bancroft,et al.  Obligatory Role of Gamma Interferon for Host Survival in a Murine Model of Infection with Burkholderia pseudomallei , 1999, Infection and Immunity.

[8]  M. Rohde,et al.  Characterization of a Murine Model of Melioidosis: Comparison of Different Strains of Mice , 1999, Infection and Immunity.

[9]  Richard A. Moore,et al.  Efflux-Mediated Aminoglycoside and Macrolide Resistance in Burkholderia pseudomallei , 1999, Antimicrobial Agents and Chemotherapy.

[10]  D. DeShazer,et al.  Current studies on the pathogenesis of melioidosis. , 1999, Microbes and infection.

[11]  D. Andersson,et al.  Novel ribosomal mutations affecting translational accuracy, antibiotic resistance and virulence of Salmonella typhimurium , 1999, Molecular microbiology.

[12]  D. Waag,et al.  Comparative efficacy of a Coxiella burnetii chloroform:methanol residue (CMR) vaccine and a licensed cellular vaccine (Q-Vax) in rodents challenged by aerosol. , 1997, Vaccine.

[13]  R. Carlyon,et al.  Mutagenesis of Burkholderia pseudomallei with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene , 1997, Journal of bacteriology.

[14]  Takayuki Ezaki,et al.  Proposal of Burkholderia gen. nov. and Transfer of Seven Species of the Genus Pseudomonas Homology Group II to the New Genus, with the Type Species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. , 1992, Microbiology and immunology.

[15]  K. Noble,et al.  ENHANCEMENT OF VIRULENCE OF MALLEOMYCES PSEUDOMALLEI , 1956, Journal of bacteriology.

[16]  A. C. Guyton,et al.  Measurement of the respiratory volumes of laboratory animals. , 1947, The American journal of physiology.

[17]  L. Reed,et al.  A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS , 1938 .

[18]  Ken Alibek,et al.  Biohazard: The Chilling True Story of the Largest Covert Biological Weapons Program in the World--Told from the Inside by the Man Who Ran It , 1999 .