Astaxanthin hyperproduction by Phaffia rhodozyma (now Xanthophyllomyces dendrorhous) with raw coconut milk as sole source of energy

Natural carbon sources, such as those present in cane sugar molasses and grape juice, promote the synthesis of astaxanthin in different Phaffia rhodozyma yeasts. One of these, coconut milk, has a very rich nutrient composition. The aim of this work was to investigate the utility of coconut milk as sole source of energy for astaxanthin pigment production by P. rhodozyma strains. Currently, coconut pulp is widely used in industrial processes in Mexico for the production of shampoos, candies, food, etc. However, coconut milk is a waste product. We show that coconut milk enhances astaxanthin production. The fermentation yielded 850 μg/g yeast with the NRRL-10921 wild-type strain and 1,850 μg/g yeast with the mutated R1 strain. Production was better than reported results employing other natural carbon sources.

[1]  Feng Chen,et al.  Separation and Analysis of Carotenoids and Chlorophylls in Haematococcus lacustris by High-Performance Liquid Chromatography Photodiode Array Detection , 1997 .

[2]  J. Sedmak,et al.  Extraction and quantitation of astaxanthin from Phaffia rhodozyma , 1990 .

[3]  G. Hayman,et al.  Production of carotenoids byPhaffia rhodozyma grown on media composed of corn wet-milling co-products , 1995, Journal of Industrial Microbiology.

[4]  Robert Auerbach,et al.  Isolation and Characterization of Carotenoid Hyperproducing Mutants of Yeast by Flow Cytometry and Cell Sorting , 1991, Bio/Technology.

[5]  M. Lewis,et al.  Astaxanthin Formation by the Yeast Phaffia rhodozyma , 1979 .

[6]  N. Nishio,et al.  Influence of Oxygen and Glucose on Primary Metabolism and Astaxanthin Production by Phaffia rhodozyma in Batch and Fed-Batch Cultures: Kinetic and Stoichiometric Analysis , 1997, Applied and environmental microbiology.

[7]  S. Kilian,et al.  Selection and evaluation of astaxanthin-overproducing mutants of Phaffia rhodozyma , 1993, World journal of microbiology & biotechnology.

[8]  Miguel Olaizola,et al.  Commercial production of astaxanthin from Haematococcus pluvialis using 25,000-liter outdoor photobioreactors , 2000, Journal of Applied Phycology.

[9]  M. Baron,et al.  Culture of the astaxanthinogenic yeast Phaffia rhodozyma in low-cost media. , 1996 .

[10]  P M Bramley,et al.  Regulation of carotenoid biosynthesis. , 1988, Current topics in cellular regulation.

[11]  T. G. Villa,et al.  Mevalonic acid increases trans-astaxanthin and carotenoid biosynthesis in Phaffia rhodozyma , 1995, Biotechnology Letters.

[12]  G. An,et al.  Astaxanthin from Microbial Sources , 1991 .

[13]  R. Sokal,et al.  Biometry: The Principles and Practice of Statistics in Biological Research (2nd ed.). , 1982 .

[14]  G. An,et al.  Isolation of Phaffia rhodozyma Mutants with Increased Astaxanthin Content , 1989, Applied and environmental microbiology.

[15]  J. C. Preez,et al.  Effect of culture conditions on astaxanthin production by a mutant of Phaffia rhodozyma in batch and chemostat culture , 1994, Applied Microbiology and Biotechnology.

[16]  Sokal Rr,et al.  Biometry: the principles and practice of statistics in biological research 2nd edition. , 1981 .

[17]  F. James Rohlf,et al.  Biometry: The Principles and Practice of Statistics in Biological Research , 1969 .

[18]  Antonio M. Martin,et al.  Kinetic studies on the yeast Phaffia rhodozyma , 1995, Journal of basic microbiology.

[19]  G. L. Miller Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[20]  M. Lewis,et al.  Selection of Astaxanthin-Overproducing Mutants of Phaffia rhodozyma with β-Ionone , 1990, Applied and environmental microbiology.