Microdrop Printing of Hydrogel Bioinks into 3D Tissue‐Like Geometries

An optimized 3D inkjet printing process is demonstrated for structuring alginate into a tissue-like microvasculature capable of supporting physiological flow rates. Optimizing the reaction at the single-droplet level enables wet hydrogel droplets to be stacked, thus overcoming their natural tendancy to spread and coalesce. Live cells can be patterned using this process and it can be extended to a range of other hydrogels.

[1]  F. Lim,et al.  Microencapsulated islets as bioartificial endocrine pancreas. , 1980, Science.

[2]  A. Mikkelsen,et al.  Density distribution of calcium‐induced alginate gels. A numerical study , 1995 .

[3]  Li Yuan-hui,et al.  Diffusion of ions in sea water and in deep-sea sediments , 1974 .

[4]  E. Place,et al.  Complexity in biomaterials for tissue engineering. , 2009, Nature materials.

[5]  D J Mooney,et al.  Alginate hydrogels as synthetic extracellular matrix materials. , 1999, Biomaterials.

[6]  Katia Bertoldi,et al.  Mathematically defined tissue engineering scaffold architectures prepared by stereolithography. , 2010, Biomaterials.

[7]  Xiaofeng Cui,et al.  Application of inkjet printing to tissue engineering , 2006, Biotechnology journal.

[8]  Birgit Glasmacher,et al.  Laser printing of stem cells for biofabrication of scaffold-free autologous grafts. , 2011, Tissue engineering. Part C, Methods.

[9]  A Krogh,et al.  The number and distribution of capillaries in muscles with calculations of the oxygen pressure head necessary for supplying the tissue , 1919, The Journal of physiology.

[10]  Anthony Atala,et al.  Isolation of amniotic stem cell lines with potential for therapy , 2007, Nature Biotechnology.

[11]  W. Hennink,et al.  In situ gelling hydrogels for pharmaceutical and biomedical applications. , 2008, International journal of pharmaceutics.

[12]  Philippe Renaud,et al.  A simple pneumatic setup for driving microfluidics. , 2007, Lab on a chip.

[13]  G. Vunjak‐Novakovic,et al.  Engineering complex tissues. , 2006, Tissue engineering.

[14]  B. Derby,et al.  Delivery of human fibroblast cells by piezoelectric drop-on-demand inkjet printing. , 2008, Biomaterials.

[15]  Sarit B. Bhaduri,et al.  Drop-on-demand printing of cells and materials for designer tissue constructs , 2007 .

[16]  O. Latinovic,et al.  Structural and micromechanical characterization of type I collagen gels. , 2010, Journal of biomechanics.

[17]  P. Calvert Inkjet Printing for Materials and Devices , 2001 .

[18]  L. Niklason,et al.  Scaffold-free vascular tissue engineering using bioprinting. , 2009, Biomaterials.

[19]  Matthias P. Lutolf,et al.  Designing materials to direct stem-cell fate , 2009, Nature.

[20]  M. L. Curri,et al.  Inkjet-printed multicolor arrays of highly luminescent nanocrystal-based nanocomposites. , 2009, Small.

[21]  A. Khademhosseini,et al.  Microscale technologies for tissue engineering and biology. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[22]  S. Schiaffino,et al.  Molten droplet deposition and solidification at low Weber numbers , 1997 .

[23]  D. Mooney,et al.  Tissue engineering strategies for in vivo neovascularisation , 2002, Expert opinion on biological therapy.

[24]  P. C. Rieke,et al.  Surfaces with Reversible Hydrophilic/Hydrophobic Characteristics on Cross-linked Poly(N-isopropylacrylamide) Hydrogels , 2000 .

[25]  Hod Lipson,et al.  Direct Freeform Fabrication of Seeded Hydrogels in Arbitrary Geometries , 2022 .

[26]  A. Valero,et al.  Link between alginate reaction front propagation and general reaction diffusion theory. , 2011, Analytical chemistry.

[27]  P. Gennes Wetting: statics and dynamics , 1985 .

[28]  D. Kohane,et al.  Engineering vascularized skeletal muscle tissue , 2005, Nature Biotechnology.

[29]  Makoto Nakamura,et al.  Ink Jet Three-Dimensional Digital Fabrication for Biological Tissue Manufacturing: Analysis of Alginate Microgel Beads Produced by Ink Jet Droplets for Three Dimensional Tissue Fabrication , 2008 .

[30]  P. Calvert Printing Cells , 2007, Science.

[31]  A. Valero,et al.  Fluidic microstructuring of alginate hydrogels for the single cell niche. , 2010, Lab on a chip.

[32]  Makoto Nakamura,et al.  Development of a three-dimensional bioprinter: construction of cell supporting structures using hydrogel and state-of-the-art inkjet technology. , 2009, Journal of biomechanical engineering.