Live-cell visualization of pre-mRNA splicing with single-molecule sensitivity.

[1]  André L. Martins,et al.  Signaling pathways differentially affect RNA polymerase II initiation, pausing, and elongation rate in cells. , 2013, Molecular cell.

[2]  Robert H Singer,et al.  Single-molecule analysis of gene expression using two-color RNA labeling in live yeast , 2012, Nature Methods.

[3]  T. Kirchhausen,et al.  Dynamics of intracellular clathrin/AP1- and clathrin/AP3-containing carriers. , 2012, Cell reports.

[4]  D. Black,et al.  Transcript Dynamics of Proinflammatory Genes Revealed by Sequence Analysis of Subcellular RNA Fractions , 2012, Cell.

[5]  Bin Wu,et al.  Fluorescence fluctuation spectroscopy enables quantitative imaging of single mRNAs in living cells. , 2012, Biophysical journal.

[6]  Colin R. Parrish,et al.  Limited Transferrin Receptor Clustering Allows Rapid Diffusion of Canine Parvovirus into Clathrin Endocytic Structures , 2012, Journal of Virology.

[7]  Sanjay Tyagi,et al.  Single-Molecule Imaging of Transcriptionally Coupled and Uncoupled Splicing , 2011, Cell.

[8]  M. Carmo-Fonseca,et al.  Spliceosome assembly is coupled to RNA polymerase II dynamics at the 3′ end of human genes , 2011, Nature Structural &Molecular Biology.

[9]  Comert Kural,et al.  Actin dynamics counteract membrane tension during clathrin-mediated endocytosis , 2011, Nature Cell Biology.

[10]  C. Will,et al.  Spliceosome structure and function. , 2011, Cold Spring Harbor perspectives in biology.

[11]  D. Auboeuf,et al.  Real-time imaging of cotranscriptional splicing reveals a kinetic model that reduces noise: implications for alternative splicing regulation , 2011, The Journal of cell biology.

[12]  Bin Wu,et al.  Real-Time Observation of Transcription Initiation and Elongation on an Endogenous Yeast Gene , 2011, Science.

[13]  M. Moore,et al.  Ordered and Dynamic Assembly of Single Spliceosomes , 2011, Science.

[14]  J. Valcárcel,et al.  Reduced fidelity of branch point recognition and alternative splicing induced by the anti-tumor drug spliceostatin A. , 2011, Genes & development.

[15]  Hye Yoon Park,et al.  A transgenic mouse for in vivo detection of endogenous labeled mRNA , 2010, Nature Methods.

[16]  J. R. Chubb,et al.  Bursts and pulses: insights from single cell studies into transcriptional mechanisms. , 2010, Current opinion in genetics & development.

[17]  R. Singer,et al.  In Vivo Imaging of Labelled Endogenous β-actin mRNA During Nucleocytoplasmic Transport , 2010, Nature.

[18]  M. Carmo-Fonseca,et al.  A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination , 2010, Nucleic acids research.

[19]  M. Jurica,et al.  Spliceostatin A inhibits spliceosome assembly subsequent to prespliceosome formation , 2010, Nucleic acids research.

[20]  T. Nilsen,et al.  Expansion of the eukaryotic proteome by alternative splicing , 2010, Nature.

[21]  Aurora Pujol,et al.  PeroxisomeDB 2.0: an integrative view of the global peroxisomal metabolome , 2009, Nucleic Acids Res..

[22]  R. Padgett,et al.  Rates of in situ transcription and splicing in large human genes , 2009, Nature Structural &Molecular Biology.

[23]  Sanjay Tyagi,et al.  Imaging intracellular RNA distribution and dynamics in living cells , 2009, Nature Methods.

[24]  Lili Wan,et al.  RNA and Disease , 2009, Cell.

[25]  A. Kornblihtt,et al.  The transcriptional cycle of HIV-1 in real-time and live cells , 2007, The Journal of Cell Biology.

[26]  X. Darzacq,et al.  In vivo dynamics of RNA polymerase II transcription , 2007, Nature Structural &Molecular Biology.

[27]  M. Hagiwara,et al.  Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA , 2007, Nature Chemical Biology.

[28]  N. Daigle,et al.  LambdaN-GFP: an RNA reporter system for live-cell imaging. , 2007, Nature methods.

[29]  Michael J Rust,et al.  Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) , 2006, Nature Methods.

[30]  R. Reed,et al.  Functional coupling of RNAP II transcription to spliceosome assembly. , 2006, Genes & development.

[31]  Robert H Singer,et al.  Materials and Methods Som Text Figs. S1 to S8 References and Notes Dynamics of Single Mrnps in Nuclei of Living Cells , 2022 .

[32]  M. Hentze,et al.  Using the λN Peptide to Tether Proteins to RNAs , 2004 .

[33]  A. Kornblihtt,et al.  A slow RNA polymerase II affects alternative splicing in vivo. , 2003, Molecular cell.

[34]  M. Hentze,et al.  Y14 and hUpf3b form an NMD-activating complex. , 2003, Molecular cell.

[35]  Robert H. Singer,et al.  Single mRNA Molecules Demonstrate Probabilistic Movement in Living Mammalian Cells , 2003, Current Biology.

[36]  A. Kornblihtt,et al.  Transcriptional Activators Differ in Their Abilities to Control Alternative Splicing* , 2002, The Journal of Biological Chemistry.

[37]  D. Weil,et al.  In Vivo Kinetics of mRNA Splicing and Transport in Mammalian Cells , 2002, Molecular and Cellular Biology.

[38]  T. Maniatis,et al.  An extensive network of coupling among gene expression machines , 2002, Nature.

[39]  J. Valcárcel,et al.  Dual Function for U2AF35 in AG-Dependent Pre-mRNA Splicing , 2001, Molecular and Cellular Biology.

[40]  S. Masich,et al.  In situ transcription and splicing in the Balbiani ring 3 gene , 2001, The EMBO journal.

[41]  J. Steitz,et al.  Human Upf Proteins Target an mRNA for Nonsense-Mediated Decay When Bound Downstream of a Termination Codon , 2000, Cell.

[42]  J. Valcárcel,et al.  Evidence for Substrate-Specific Requirement of the Splicing Factor U2AF35 and for Its Function after Polypyrimidine Tract Recognition by U2AF65 , 1999, Molecular and Cellular Biology.

[43]  E. Salmon,et al.  Localization and anchoring of mRNA in budding yeast , 1999, Current Biology.

[44]  F. Grosveld,et al.  Inefficient processing impairs release of RNA from the site of transcription , 1999, The EMBO journal.

[45]  N. Proudfoot,et al.  Terminal exon definition occurs cotranscriptionally and promotes termination of RNA polymerase II. , 1999, Molecular cell.

[46]  R. Singer,et al.  Localization of ASH1 mRNA particles in living yeast. , 1998, Molecular cell.

[47]  C. Guthrie,et al.  Mechanical Devices of the Spliceosome: Motors, Clocks, Springs, and Things , 1998, Cell.

[48]  F. Grosveld,et al.  Efficient 3'-end formation of human beta-globin mRNA in vivo requires sequences within the last intron but occurs independently of the splicing reaction. , 1998, Nucleic acids research.

[49]  Lars Liljas,et al.  Crystal structure of an RNA bacteriophage coat protein–operator complex , 1994, Nature.

[50]  L. Chasin,et al.  Order of intron removal during splicing of endogenous adenine phosphoribosyltransferase and dihydrofolate reductase pre-mRNA , 1993, Molecular and cellular biology.

[51]  Y. Shimura,et al.  The role of exon sequences in splice site selection. , 1993, Genes & development.

[52]  Susan M. Berget,et al.  Are vertebrate exons scanned during splice-site selection? , 1992, Nature.

[53]  Y. Shimura,et al.  A secondary structure at the 3' splice site affects the in vitro splicing reaction of mouse immunoglobulin mu chain pre-mRNAs. , 1989, Nucleic acids research.

[54]  I. Graham,et al.  Effects of RNA secondary structure on alternative splicing of Pre-mRNA: Is folding limited to a region behind the transcribing RNA polymerase? , 1988, Cell.

[55]  Y. Osheim,et al.  Splice site selection, rate of splicing, and alternative splicing on nascent transcripts. , 1988, Genes & development.

[56]  O. Uhlenbeck,et al.  An RNA mutation that increases the affinity of an RNA-protein interaction. , 1987, Nucleic acids research.

[57]  L. J. Korn,et al.  Regulation of differential processing of mouse immunoglobulin mu heavy-chain mRNA. , 1987, Nucleic acids research.

[58]  P. Keohavong,et al.  Splicing of the E2A premessenger RNA of adenovirus serotype 2. Multiple pathways in spite of excision of the entire large intron. , 1986, Journal of molecular biology.

[59]  N. Franklin.  Conservation of genome form but not sequence in the transcription antitermination determinants of bacteriophages λ, φ21 and P22☆ , 1985 .

[60]  N. Franklin.  Conservation of genome form but not sequence in the transcription antitermination determinants of bacteriophages lambda, phi 21 and P22. , 1985, Journal of molecular biology.