Registration of sugar beet genetic stocks FC308 (PI701378) and FC309 (PI700990)

Sugar beet (Beta vulgaris L.) genetic stock lines FC308 (PI701378) and FC309 (PI700990) are two highly homozygous and phenotypically homogenous sources of resistance to two sugar beet pests. FC308 is resistant to sugar beet cyst nematode (Heterodera schachtii), but susceptible to Fusarium yellows, while FC309 is Fusarium yellows resistant, but sugar beet cyst nematode susceptible. These two populations were developed by the USDA-ARS in Fort Collins, CO, derived from unreleased USDA-ARS Salinas germplasm lines. Multiple field and greenhouse trials for Fusarium yellows and nematode resistance confirmed the uniform phenotypes of both lines for each disease. To enable molecular breeders to rapidly screen proprietary markers in silico, gene space assemblies for each line were also developed. Genome sequencing of individual plants from each line, as well as pooled sequencing of sub-populations of both lines indicated fixation of a previously reported genetic variant linked to sugar beet cyst nematode resistance in FC308. However, two previously reported variants linked to Fusarium resistance appear to be unlinked to the resistance found in FC309. Collectively, these two new genetic stocks should prove useful as sources of resistance to Fusarium yellows and sugar beet cyst nematode, as model genetic backgrounds for studying plant-pathogen interactions, and for mapping the resistance genes present in these lines.

[1]  S. B. Mahmoudi,et al.  Molecular insights into the compatible and incompatible interactions between sugar beet and the beet cyst nematode , 2020, BMC plant biology.

[2]  K. Demeestere,et al.  Ascorbate Oxidase Induces Systemic Resistance in Sugar Beet Against Cyst Nematode Heterodera schachtii , 2020, Frontiers in Plant Science.

[3]  K. Richardson.  Registration of Sugar Beet Germplasm Lines CN921‐515 and CN921‐516 with Sugar Beet Cyst Nematode Resistance from Beta vulgaris subsp. maritima , 2018 .

[4]  Han Fang,et al.  GenomeScope: Fast reference-free genome profiling from short reads , 2016, bioRxiv.

[5]  Z. Fang,et al.  Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection , 2017, Front. Plant Sci..

[6]  L. Panella,et al.  Molecular markers for improving control of soil-borne pathogen Fusarium oxysporum in sugar beet , 2017, Euphytica.

[7]  L. Panella,et al.  Molecular markers for improving control of soil-borne pathogen Fusarium oxysporum in sugar beet , 2017, Euphytica.

[8]  L. Panella,et al.  Identification and Validation of a SNP Marker Linked to the Gene HsBvm-1 for Nematode Resistance in Sugar Beet , 2015, Plant Molecular Biology Reporter.

[9]  K. Richardson.  Registration of Sugarbeet Germplasm Lines CN12-446, CN12-770, and CN72-652 with Resistance to Sugarbeet Cyst Nematode , 2012 .

[10]  J. Anderson.  Marker-assisted selection for Fusarium head blight resistance in wheat. , 2008, International journal of food microbiology.

[11]  H. Atamian,et al.  Marker-Assisted Selection of Tomato Genotypes with the I-2 Gene for Resistance to Fusarium oxysporum f. sp. lycopersici Race 2. , 2007, Plant disease.

[12]  P. Urwin,et al.  Molecular aspects of cyst nematodes. , 2005, Molecular plant pathology.

[13]  R. Lewellen,et al.  Performance of sugarbeet cyst nematode resistant cultivars and a search for sources of resistance , 2005 .

[14]  L. Hanson,et al.  Fusarium species causing Fusarium yellows of sugarbeet. , 2004 .

[15]  C. Jung,et al.  Sporamin-mediated resistance to beet cyst nematodes (Heterodera schachtii Schm.) is dependent on trypsin inhibitory activity in sugar beet (Beta vulgaris L.) hairy roots , 2003, Plant Molecular Biology.

[16]  C. Alabouvette,et al.  Fusarium oxysporum and its biocontrol. , 2003, The New phytologist.

[17]  K. Abada.  Fungi causing damping-off and root-rot on sugar-beet and their biological control with Trichoderma harzianum , 1994 .

[18]  D. Cooke.  The effect of beet cyst nematode, Heterodera schachtii, on the yield of sugar beet in organic soils , 1991 .

[19]  L. Panella,et al.  Response of Sugarbeet Lines to Isolates of Fusarium oxysporum f. sp. Betae from the United States , 2009 .

[20]  P. Miklas,et al.  Marker-Assisted Selection , 2005 .

[21]  E. Ruppel.  Pathogenicity of Fusarium spp. from diseased sugar beets and variation among sugar beet isolates of F. oxysporum. , 1991 .

[22]  C. Rush,et al.  Etiology of a Root Rot Disease of Sugar Beet in Texas , 1989 .

[23]  A. L. E.,et al.  Response of Sugarbeet Lines to Isolates of Fusarium oxysporum f . sp . Betae from the United States Hanson , , 2022 .