Effect of Clotting Duration and Temperature on BDNF Measurement in Human Serum

Brain-derived neurothrophic factor (BDNF) is a neurotrophin expressed in different tissues and cells, including neurons, endothelial cells, leukocytes, megakaryocytes and platelets. Modifications of BDNF in plasma and/or in serum are associated with neurodegenerative and psychiatric disorders, cardiovascular diseases, metabolic syndrome and with mortality risk. Indeed, changes in blood levels of BDNF may reflect those of its tissue of origin and/or promote pathological dysfunctions. The measurement of BDNF amount in plasma or in serum has been characterized with particular attention in the impact of different anti-coagulants, clotting duration, temperature (≤21 °C) and delay in blood sample centrifugation as well as in stability of storage. However, the influences of normothermic conditions (37 °C) and of clotting duration on BDNF levels in human serum have not been investigated yet. Here, we showed that time and temperature during serum preparation could be taken into consideration to assess the association and/or impact of BDNF levels in the occurrence of pathological conditions.

[1]  J. Kaiser,et al.  Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations , 2017, International journal of molecular sciences.

[2]  Isaac Karimi,et al.  The interrelationship of metabolic syndrome and neurodegenerative diseases with focus on brain-derived neurotrophic factor (BDNF): Kill two birds with one stone , 2017, Metabolic Brain Disease.

[3]  D. Baldassarre,et al.  Effect of cigarette smoke on monocyte procoagulant activity: Focus on platelet-derived brain-derived neurotrophic factor (BDNF) , 2017, Platelets.

[4]  L. Wilkins.  Circulating Brain‐Derived Neurotrophic Factor Concentrations and the Risk of Cardiovascular Disease in the Community , 2016, Journal of the American Heart Association : Cardiovascular and Cerebrovascular Disease.

[5]  Undurti N. Das,et al.  Brain-derived neurotrophic factor and its clinical implications , 2015, Archives of medical science : AMS.

[6]  E. Tongiorgi,et al.  A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays , 2015, Scientific Reports.

[7]  L. Bocchio-Chiavetto,et al.  Influence of clotting duration on brain-derived neurotrophic factor (BDNF) dosage in serum. , 2014, BioTechniques.

[8]  Norio Sugawara,et al.  Preanalysis Storage Conditions Influence the Measurement of Brain-Derived Neurotrophic Factor Levels in Peripheral Blood , 2014, Neuropsychobiology.

[9]  Edward J. Wild,et al.  Brain-Derived Neurotrophic Factor in Patients with Huntington's Disease , 2011, PloS one.

[10]  S. Negrotto,et al.  Hyperthermia inhibits platelet hemostatic functions and selectively regulates the release of alpha‐granule proteins , 2011, Journal of thrombosis and haemostasis : JTH.

[11]  L. Ferrucci,et al.  Circulating Brain-Derived Neurotrophic Factor and Indices of Metabolic and Cardiovascular Health: Data from the Baltimore Longitudinal Study of Aging , 2010, PloS one.

[12]  Y. Béjot,et al.  Serum brain‐derived neurotrophic factor and platelet activation evaluated by soluble P‐selectin and soluble CD‐40‐ligand in patients with acute myocardial infarction , 2009, Fundamental & clinical pharmacology.

[13]  K. Polderman,et al.  Mechanisms of action, physiological effects, and complications of hypothermia , 2009, Critical care medicine.

[14]  M. Vinberg,et al.  Measurements of brain-derived neurotrophic factor: Methodological aspects and demographical data , 2007, Brain Research Bulletin.

[15]  C. Sirtori,et al.  Effect of n‐3 fatty acids on carotid atherosclerosis and haemostasis in patients with combined hyperlipoproteinemia: A double‐blind pilot study in primary prevention , 2006, Annals of medicine.

[16]  Norbert Maurer,et al.  Room Temperature Activates Human Blood Platelets , 2001, Laboratory Investigation.

[17]  Hans Lassmann,et al.  Activated Human T Cells, B Cells, and Monocytes Produce Brain-derived Neurotrophic Factor In Vitro and in Inflammatory Brain Lesions: A Neuroprotective Role of Inflammation? , 1999, The Journal of experimental medicine.

[18]  William E Grizzle,et al.  Standard operating procedures for serum and plasma collection: early detection research network consensus statement standard operating procedure integration working group. , 2009, Journal of proteome research.