Identification of a NIPSNAP homologue as host cell target for Salmonella virulence protein SpiC

Salmonella enterica uses a type III secretion system encoded by SPI‐2 to target specific virulence factors into the host cytosol of macrophages to inhibit the phagosomal‐lysosomal maturation pathway. This ensures survival of Salmonella inside its intracellular niche, the Salmonella‐containing vacuole (SCV). One such virulence factor is SpiC, which was previously shown to interfere with intracellular vesicular trafficking. In this study we have used a yeast two‐hybrid assay to identify a NIPSNAP homologue as host cell target for SpiC that we have termed TassC. In vitro and in vivo co‐purification of SpiC and TassC confirm the specificity of this interaction. Suppression of TassC production compensates a SpiC production deficit and allows spiC–Salmonella to survive within macrophages at levels comparable to wild‐type Salmonella. We hypothesize that TassC is a host cell factor that determines vesicular trafficking in macrophages and is inactivated by Salmonella SpiC.

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