Corneal epithelial glucose utilization.

Determination of in vivo glucose consumption in rabbits shows a utilization of at least 38μg/sq cm/hr for the whole cornea. Using a glued-on chamber, the epithelial glucose consumption is 16.9μg/sq cm/hr in oxygen, and 24.8 μg/sq cm/ hr in nitrogen. The results indicate that the limbal supply of glucose of 7.2 μg/sq cm/hr is inadequate to support the epithelium. Lactate production when the epithelium is present is higher in the presence of nitrogen that in the presence of oxygen, confirming that the shift to anaerobic glycolysis occurs mainly in the epithelium. In addition, it is found that the epithelial glycogen depot is rapidly mobilized by relatively gentle manipulation.

[1]  R B Mandell,et al.  Critical oxygen tension at the corneal surface. , 1970, Archives of ophthalmology.

[2]  L. Horberg.  Quaternary Volcanic Ash in Southern Alberta, Canada. , 1952, Science.

[3]  M. Reim,et al.  Steady state levels of glucose in the different layers of the cornea, aqueous humor, blood and tears in vivo. , 1967, Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde.

[4]  M. Riley.  Aerobic glycolysis in the ox cornea. , 1969, Experimental eye research.

[5]  G. K. Smelser,et al.  Importance of atmospheric oxygen for maintenance of the optical properties of the human cornea. , 1952, Science.

[6]  H. Herrmann,et al.  Exploratory studies on corneal metabolism. , 1948, Bulletin of the Johns Hopkins Hospital.

[7]  A. Deroetth.  Respiration of the cornea. , 1950 .

[8]  G. K. Smelser.  Relation of factors involved in maintenance of optical properties of cornea to contact-lens wear. , 1952, A.M.A. archives of ophthalmology.

[9]  J. Friend,et al.  Corneal glucose concentration. Flux in the presence and absence of epithelium. , 1971, Archives of ophthalmology.

[10]  D. Maurice,et al.  The Cornea and Sclera , 1962 .

[11]  M. Langham.  Glycolysis in the cornea of the rabbit , 1954, The Journal of physiology.

[12]  M V Riley,et al.  Glucose and oxygen utilization by the rabbit cornea. , 1969, Experimental eye research.

[13]  J. Friend,et al.  Anterior segment necrosis in rabbits. Metabolic and histologic changes. , 1971, Archives of ophthalmology.

[14]  J. Friend,et al.  Corneal glucose flux. II. Its response to anterior chamber blockade and endothelial damage. , 1971, Archives of ophthalmology.

[15]  Burns Rp.  Meesman's corneal dystrophy. , 1968 .

[16]  J. Friend,et al.  The anterior segments of rabbits after alkali burns. Metabolic and histologic alterations. , 1971, Archives of ophthalmology.