Quantifying external and internal collagen organization from Stokes-vector-based second harmonic generation imaging polarimetry

Collagen organization has been analyzed at both external and internal scales by combining Stokes-vector polarimetry and second harmonic generation microscopy. A significant linear relationship between the diattenuation and the external collagen organization was found. The dominant orientation of the collagen fibers was found to run parallel to the axis of diattenuation. Information on the collagen chirality was obtained from the circular dichroism, which showed also a strong dependence with the internal collagen organization. The results show that certain polarimetric parameters might be useful to extract quantitative information and characterize collagen arrangement.

[1]  J. Bueno,et al.  Measurement of parameters of polarization in the living human eye using imaging polarimetry , 2000, Vision Research.

[2]  Antonello De Martino,et al.  Determination of collagen fiber orientation in histological slides using Mueller microscopy and validation by second harmonic generation imaging. , 2014, Optics express.

[3]  Juan M Bueno,et al.  Polarization response of second-harmonic images for different collagen spatial distributions , 2016, Journal of biomedical optics.

[4]  Monal R. Mehta,et al.  Fourier transform-second-harmonic generation imaging of biological tissues. , 2009, Optics express.

[5]  J. Baum,et al.  Folding of peptide models of collagen and misfolding in disease. , 1999, Current opinion in structural biology.

[6]  M. Kauranen,et al.  Chiral imaging of collagen by second-harmonic generation circular dichroism , 2013, Biomedical optics express.

[7]  Chen-Yuan Dong,et al.  Determination of collagen nanostructure from second-order susceptibility tensor analysis. , 2011, Biophysical journal.

[8]  Sophie Brasselet,et al.  Polarization-resolved nonlinear microscopy: application to structural molecular and biological imaging , 2011 .

[9]  Gaël Latour,et al.  In vivo structural imaging of the cornea by polarization-resolved second harmonic microscopy , 2011, Biomedical optics express.

[10]  P. Artal,et al.  Average double-pass ocular diattenuation using foveal fixation , 2008 .

[11]  Francisco J. Ávila,et al.  Analysis and quantification of collagen organization with the structure tensor in second harmonic microscopy images of ocular tissues. , 2015, Applied optics.

[12]  Fu-Jen Kao,et al.  Stokes vector based polarization resolved second harmonic microscopy of starch granules , 2013, Biomedical optics express.

[13]  Paul J Campagnola,et al.  Second-harmonic generation circular dichroism studies of osteogenesis imperfecta. , 2012, Optics letters.

[14]  Masood Samim,et al.  Double Stokes Mueller polarimetry of second-harmonic generation in ordered molecular structures , 2015 .

[15]  Sergey Plotnikov,et al.  Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure , 2012, Nature Protocols.

[16]  G. Baffet,et al.  Orientation fields of nonlinear biological fibrils by second harmonic generation microscopy , 2008, Journal of microscopy.

[17]  Virginijus Barzda,et al.  Nonlinear optical properties of type I collagen fibers studied by polarization dependent second harmonic generation microscopy. , 2011, The journal of physical chemistry. B.

[18]  Patrick Stoller,et al.  Quantitative second-harmonic generation microscopy in collagen. , 2003, Applied optics.

[19]  Watt W Webb,et al.  Interpreting second-harmonic generation images of collagen I fibrils. , 2005, Biophysical journal.

[20]  Maris Ozolinsh,et al.  Degree of polarization as an objective method of estimating scattering. , 2004, Journal of the Optical Society of America. A, Optics, image science, and vision.

[21]  D. Hulmes,et al.  Building collagen molecules, fibrils, and suprafibrillar structures. , 2002, Journal of structural biology.

[22]  Marie-Claire Schanne-Klein,et al.  Polarization-resolved Second Harmonic microscopy in anisotropic thick tissues. , 2010, Optics express.

[23]  Jianxin Chen,et al.  Theoretical simulation study of linearly polarized light on microscopic second-harmonic generation in collagen type I. , 2009, Journal of biomedical optics.

[24]  Isaac Freund,et al.  Second harmonic generation in collagen , 1979 .

[25]  Marie Guilbert,et al.  Microscopic structural study of collagen aging in isolated fibrils using polarized second harmonic generation. , 2012, Journal of biomedical optics.

[26]  Beop-Min Kim,et al.  Polarization-dependent optical second-harmonic imaging of a rat-tail tendon. , 2002, Journal of biomedical optics.

[27]  P. Török,et al.  Revealing molecular structure and orientation with Stokes vector resolved second harmonic generation microscopy. , 2014, Methods.

[28]  Paul Campagnola,et al.  Second harmonic generation imaging microscopy: applications to diseases diagnostics. , 2011, Analytical chemistry.

[29]  Francisco J. Ávila,et al.  Polarization dependence of aligned collagen tissues imaged with second harmonic generation microscopy , 2015, Journal of biomedical optics.

[30]  Oscar del Barco,et al.  Second harmonic generation signal in collagen fibers: role of polarization, numerical aperture, and wavelength. , 2012, Journal of biomedical optics.

[31]  Chen-Yuan Dong,et al.  Discrimination of collagen in normal and pathological skin dermis through second-order susceptibility microscopy. , 2009, Optics express.

[32]  D. Marsh.  Dichroic ratios in polarized Fourier transform infrared for nonaxial symmetry of beta-sheet structures. , 1997, Biophysical journal.

[33]  Masood Samim,et al.  Second harmonic generation double stokes Mueller polarimetric microscopy of myofilaments. , 2016, Biomedical optics express.

[34]  Sophie Brasselet,et al.  Influence of birefringence on polarization resolved nonlinear microscopy and collagen SHG structural imaging. , 2010, Optics express.

[35]  S. Stanciu,et al.  Improved quantification of collagen anisotropy with polarization‐resolved second harmonic generation microscopy , 2017, Journal of biophotonics.

[36]  Juan M. Bueno,et al.  Polarimetry in the human eye using an imaging linear polariscope , 2002 .

[37]  Garth J Simpson,et al.  Chirality in nonlinear optics. , 2009, Annual review of physical chemistry.

[38]  Rod Seeley,et al.  Anatomy & Physiology 6th edition Chapter Quiz , 2003 .

[39]  Garth J. Simpson,et al.  Modular ellipsometric approach for mining structural information from nonlinear optical polarization analysis , 2005 .