Cytomorphometric analysis of squames obtained from normal oral mucosa and lesions of oral leukoplakia and squamous cell carcinoma.

Cell and nuclear diameters (CD and ND) were measured in squames obtained from normal buccal mucosa and lesions of oral leukoplakia and squamous carcinoma (SCC) also from buccal mucosa. The study groups consisted of Group 1: normal buccal mucosa (n = 40); Group 2: lesions with no epithelial dysplasia (n = 58); Group 3: lesions with epithelial dysplasia (n = 27); and Group 4: SCC lesions (n = 51). The mean CD and ND values were: Group 1: 51.78 (+/- 0.11) and 8.36 (+/- 0.49); Group 2: 45.73 (+/- 0.16) and 8.31 (+/- 0.68); Group 3: 41.32 (+/- 0.13) and 9.04 (+/- 0.46); Group 4: 38.58 (+/- 0.11) and 10.10 (+/- 0.56) microns, respectively. Correlation between the ND and CD was positive for Group 1 (r = 0.78, P < 0.05) and Group 2 (r = 0.33, P < 0.05). There were no significant correlations in Groups 3 and 4. ANOVA showed significant differences (P < 0.05) for CD between all four groups. Except between Groups 1 and 2, the ND was significantly different (P < 0.05) between all groups. The results indicate that ND and CD could possibly be sensitive parameters in the diagnosis of oral premalignant and malignant lesions.

[1]  G. Ogden,et al.  Evidence of field change in oral cancer. , 1990, The British journal of oral & maxillofacial surgery.

[2]  J G Cowpe,et al.  Quantitative exfoliative cytology of normal buccal mucosa: effect of smoking. , 1990, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology.

[3]  J G Cowpe,et al.  The effect of distant malignancy upon quantitative cytologic assessment of normal oral mucosa , 1990, Cancer.

[4]  G. Ogden,et al.  Quantitative cytophotometric analysis as an aid to the detection of recurrent oral cancer. , 1989, The British journal of oral & maxillofacial surgery.

[5]  J. Cowpe,et al.  Quantitative Exfoliative Cytology of Abnormal Oral Mucosal Smears , 1988, Journal of the Royal Society of Medicine.

[6]  H. J. G. Gundersen,et al.  The new stereological tools: Disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis , 1988, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[7]  H. J. G. GUNDERSEN,et al.  Some new, simple and efficient stereological methods and their use in pathological research and diagnosis , 1988, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[8]  J. Cowpe,et al.  Quantitative Exfoliative Cytology of Normal Oral Squames: An Age, Site and Sex-Related Survey , 1985, Journal of the Royal Society of Medicine.

[9]  J. Cowpe.  Quantitative Exfoliative Cytology of Normal and Abnormal Oral Mucosal Squames: Preliminary Communication , 1984, Journal of the Royal Society of Medicine.

[10]  C. Smith,et al.  Stereological analysis of histological parameters in experimental premalignant hamster cheek pouch epithelium , 1980, The Journal of pathology.

[11]  Kramer Ir,et al.  Definition of leukoplakia and related lesions: an aid to studies on oral precancer. , 1978 .

[12]  B. Kissin,et al.  Oral cytologic patterns in relation to smoking habits. Some epithelial, microfloral, and leukocytic characteristics. , 1976, Oral surgery, oral medicine, and oral pathology.

[13]  C. Reddy,et al.  Correlative study of exfoliative cytology and histopathology of oral carcinomas. , 1975, Journal of oral surgery.

[14]  A. Pappelis,et al.  Cellular and nuclear dry mass and area changes during human oral mucosa cell development. , 1973, Acta cytologica.

[15]  T C Folsom,et al.  Oral exfoliative study. Review of the literature and report of a three-year study. , 1972, Oral surgery, oral medicine, and oral pathology.

[16]  D M Humphreys,et al.  Quantitative morphology of cancer cells. , 1972, Acta histochemica.

[17]  E. Dabelsteen,et al.  The Limitations of Exfoliative Cytology for the Detection of Epithelial Atypia in Oral Leukoplakias , 1971, British Journal of Cancer.

[18]  Goldby Jw,et al.  NUCLEAR AND CELLULAR SIZE VARIATIONS IN CLINICALLY NORMAL EXFOLIATED BUCCAL MUCOSAL CELLS. , 1964 .

[19]  M. M. Boddington.  CHANGES IN BUCCAL CELLS IN THE ANAEMIAS , 1959, Journal of clinical pathology.

[20]  J. Reagan,et al.  Analytical study of the cells in cervical squamous-cell cancer. , 1957, Laboratory investigation; a journal of technical methods and pathology.