Effect of water trophic status on bio-optical properties and productive characteristics of phytoplankton of the Black Sea coastal waters near Sevastopol

ABSTRACT Spectral bio-optical properties and phytoplankton photosynthetic parameters were measured in Black Sea coastal waters near Sevastopol in May–July 2020–2021. Chlorophyll a concentration (Chl-a) differed by more than one order of magnitude between stations (0.20–5.6 mg/m3) due to the effect of the Chyornaya river runoff. Light absorption coefficients by all optically active components as well as total non-water absorption coefficients co-varied with Chl-a, which reflected a link between trophic status and water optical properties. An increase in total non-water absorption resulted in a shift of the maximum of downwelling irradiance to the longer wavelength (from 511 nm to 567 nm). Phytoplankton photosynthetic parameters were depth-dependent variables due to phytoplankton acclimations to light intensity without dependence on water trophic status. Gross primary production was estimated by the full spectral approach. Primary production (1.3–31 mgC/m3/h) co-varied with Chl-a. Chlorophyll a specific photosynthesis rate (4.3–8.0 mgC/mgChl/h) varied by about twice at the sea surface but no dependence on Chl-a was revealed.

[1]  I. Berman‐Frank,et al.  Single-Turnover Variable Chlorophyll Fluorescence as a Tool for Assessing Phytoplankton Photosynthesis and Primary Productivity: Opportunities, Caveats and Recommendations , 2021, Frontiers in Marine Science.

[2]  H. M. Sosik,et al.  Dependence of the Photosynthetic Quantum Yield on Phytoplankton Light Absorption: Equations for Assessing Primary Production in the Black Sea , 2021, Physical Oceanography.

[3]  B. Cornils.  single turnover , 2020, Catalysis from A to Z.

[4]  Robert J. W. Brewin,et al.  Primary Production, an Index of Climate Change in the Ocean: Satellite-Based Estimates over Two Decades , 2020, Remote. Sens..

[5]  T. Churilova,et al.  Correction of the Chlorophyll a Fluorescence Quenching in the Sea Upper Mixed Layer: Development of the Algorithm , 2020, Physical Oceanography.

[6]  T. Churilova,et al.  Phytoplankton Bloom and Photosynthetically Active Radiation in Coastal Waters , 2020 .

[7]  A. Garmashov.  HYDROLOGICAL CHARACTERISTICS OF SEVASTOPOL BAY , 2019, 19th International Multidisciplinary Scientific GeoConference SGEM2019, Water Resources. Forest, Marine and Ocean Ecosystems.

[8]  A. Rubin,et al.  Chlorophyll fluorescence induction and relaxation system for the continuous monitoring of photosynthetic capacity in photobioreactors. , 2019, Physiologia plantarum.

[9]  T. Efimova,et al.  Dynamics in pigment concentration and light absorption by phytoplankton, non-algal particles and colored dissolved organic matter in the Black Sea coastal waters (near Sevastopol) , 2018, Atmospheric and Ocean Optics.

[10]  H. M. Sosik,et al.  Phytoplankton light absorption in the deep chlorophyll maximum layer of the Black Sea , 2018, European Journal of Remote Sensing.

[11]  T. Efimova,et al.  Light absorption coefficients by phytoplankton pigments, suspended particles and colored dissolved organic matter in the Crimea coastal water (the Black sea) in June 2016 , 2017, Atmospheric and Ocean Optics.

[12]  T. Khmara,et al.  Comparative Assessment of the Trophic Level of the Sevastopol and Yuzhnaya Bays Using E-TRIX Index , 2017 .

[13]  T. Churilova,et al.  Light Absorption by Phytoplankton in the Upper Mixed Layer of the Black Sea: Seasonality and Parametrization , 2017, Front. Mar. Sci..

[14]  I. Dovgal,et al.  Spectral Approach to Assessment of Phytoplankton Photosynthesis Rate in the Black Sea Based on Satellite Information: Methodological Aspects of the Regional Model Development , 2016 .

[15]  Т. В. Ефимова,et al.  Спектральный подход к оценке скорости фотосинтеза фитопланктона в Черном море по спутниковой информации: методологические аспекты развития региональной модели , 2016 .

[16]  T. Churilova,et al.  A regional algorithm for separating light absorption by chlorophyll-a and coloured detrital matter in the Black Sea, using 480–560 nm bands from ocean colour scanners , 2016 .

[17]  Olga Krivenko,et al.  Photosynthetically available radiation on surface of the Black Sea based on ocean color data , 2015, Atmospheric and Ocean Optics.

[18]  R. Geider,et al.  Predicting the Electron Requirement for Carbon Fixation in Seas and Oceans , 2013, PloS one.

[19]  D. Siegel,et al.  The global distribution and dynamics of chromophoric dissolved organic matter. , 2013, Annual review of marine science.

[20]  J. Louda,et al.  Microalgal pigment ratios in relation to light intensity: implications for chemotaxonomy , 2010 .

[21]  Stephen Wright,et al.  An alternative approach. , 2010, Nursing standard (Royal College of Nursing (Great Britain) : 1987).

[22]  H. Sosik,et al.  A spectral model of underwater irradiance in the Black Sea , 2009 .

[23]  J. D. Ritchie,et al.  Absorption spectral slopes and slope ratios as indicators of molecular weight, source, and photobleaching of chromophoric dissolved organic matter , 2008 .

[24]  J. Serôdio,et al.  Relationship of rapid light curves of variable fluorescence to photoacclimation and non-photochemical quenching in a benthic diatom , 2008 .

[25]  Martin Ostrowski,et al.  Diversity and evolution of phycobilisomes in marine Synechococcus spp.: a comparative genomics study , 2007, Genome Biology.

[26]  Jon Brodie,et al.  Spatial and temporal patterns of near-surface chlorophyll a in the Great Barrier Reef lagoon , 2007 .

[27]  C. Mobley,et al.  Optical modeling of ocean waters: Is the case 1 - case 2 classification still useful? , 2004 .

[28]  M. A. Evdoshenko,et al.  Application of SeaWiFS data for studying variability of bio-optical characteristics in the Barents, Black and Caspian Seas , 2004 .

[29]  Dariusz Stramski,et al.  Variations in the light absorption coefficients of phytoplankton, nonalgal particles, and dissolved organic matter in coastal waters around Europe , 2003 .

[30]  Satoru Taguchi,et al.  Variability in chlorophyll a specific absorption coefficient in marine phytoplankton as a function of cell size and irradiance , 2002 .

[31]  Hugh L. MacIntyre,et al.  PHOTOACCLIMATION OF PHOTOSYNTHESIS IRRADIANCE RESPONSE CURVES AND PHOTOSYNTHETIC PIGMENTS IN MICROALGAE AND CYANOBACTERIA 1 , 2002 .

[32]  Attilio Rinaldi,et al.  Characterization of the trophic conditions of marine coastal waters with special reference to the NW Adriatic Sea: proposal for a trophic scale, turbidity and generalized water quality index , 1998 .

[33]  A. Obata,et al.  Global verification of critical depth theory for phytoplankton bloom with climatological in situ temperature and satellite ocean color data , 1996 .

[34]  P. Falkowski,et al.  Nitrogen- and irradiance-dependent variations of the maximum quantum yield of carbon fixation in eutrophic, mesotrophic and oligotrophic marine systems , 1996 .

[35]  Stelvio Tassan,et al.  An alternative approach to absorption measurements of aquatic particles retained on filters , 1995 .

[36]  H. Claustre,et al.  Variability in the chlorophyll‐specific absorption coefficients of natural phytoplankton: Analysis and parameterization , 1995 .

[37]  E. Sakshaug,et al.  Photobiological studies of Skeletonema costatum adapted to spectrally different light regimes , 1993 .

[38]  B. G. Mitchell,et al.  Algorithms for determining the absorption coefficient for aquatic particulates using the quantitative filter technique , 1990, Defense, Security, and Sensing.

[39]  Karen S. Baker,et al.  Bio‐optical modeling of photosynthetic production in coastal waters , 1989 .

[40]  Dale A. Kiefer,et al.  Chlorophyll α specific absorption and fluorescence excitation spectra for light-limited phytoplankton , 1988 .

[41]  Karen S. Baker,et al.  Oceanic primary production estimates from measurements of spectral irradiance and pigment concentrations , 1987 .

[42]  C. Lorenzen,et al.  DETERMINATION OF CHLOROPHYLL AND PHEO‐PIGMENTS: SPECTROPHOTOMETRIC EQUATIONS1 , 1967 .

[43]  A. Lillebø,et al.  Coastal Lagoons in Europe: Integrated Water Resource Strategies , 2015 .

[44]  R. Majchrowski,et al.  Quantum yield of photosynthesis in the Baltic: a new mathematical expression for remote sensing applications , 2007 .

[45]  J. B. Rosario,et al.  The Effect of Fresh Water Runoff on the Distribution of Dissolved Inorganic Nutrients and Plankton in the Bocas del Toro Archipelago, Caribbean Panama , 2005 .

[46]  Ulrich Schreiber,et al.  Pulse-Amplitude-Modulation (PAM) Fluorometry and Saturation Pulse Method: An Overview , 2004 .

[47]  H. Sosik,et al.  Variability of Photosynthetic Parameters of the Surface Phytoplankton in the Black Sea , 2003 .

[48]  Dennis A. Hansell,et al.  Biogeochemistry of marine dissolved organic matter , 2002 .

[49]  R. Bidigare,et al.  Pigment absorption and quantum yields in the Arabian Sea , 2000 .

[50]  P. Falkowski,et al.  Aquatic Photosynthesis: Second Edition , 1997 .

[51]  J. Kirk,et al.  Light and Photosynthesis in Aquatic Ecosystems , 1995 .

[52]  W. Bilger,et al.  Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis , 1994 .

[53]  Motoaki Kishino,et al.  Estimation of the spectral absorption coefficients of phytoplankton in the sea , 1985 .

[54]  Trevor Platt,et al.  Photoinhibition of photosynthesis in natural assemblages of marine phytoplankton , 1980 .

[55]  G. F. Humphrey,et al.  New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton , 1975 .