CERTIFICATION REPORT The certification of different mass fractions of DP-ØØ4114-3 in maize seed powderCertified Reference Materials ERM®-BF439a, ERM®-BF439b, ERM®-BF439c, ERM®-BF439d and ERM®-BF439e

This report describes the production of a set of Certified Reference Materials (CRMs) ERM-BF432a, b, c and d, matrix materials certified for their DAS-68416-4 mass fractions. The material has been produced following ISO Guide 34:2009 [1]. Genetically modified (GM) seeds of the soya event DAS-68416-4 and of a non-GM soya variety were ground to obtain GM and non-GM base powders. Gravimetric mixtures of non-GM and GM soya powder were prepared by dry-mixing. Between unit-heterogeneity has been quantified and stability during dispatch and storage have been assessed in accordance with ISO Guide 35:2006 [2]. The certified value was obtained from the gravimetric preparations, taking into account the purity of the base materials and their water mass fraction. The certified values were confirmed by event-specific real- time PCR as independent verification method (measurements within the scope of accreditation to ISO/IEC 17025:2005 [3]). Uncertainties of the certified values were calculated in compliance with the Guide to the Expression of Uncertainty in Measurement (GUM) [4] and include uncertainties related to possible heterogeneity, instability, and characterisation. The materials are intended for the calibration or quality control of methods. As any reference material, they can also be used for control charts or validation studies. The CRMs are available in glass vials containing at least 1 g of dried soya seed powder and closed under argon atmosphere. The minimum amount of sample to be used is 500 mg. The CRM has been accepted as European Reference Material (ERM) after peer evaluation by the partners of the European Reference Materials consortium. The following values were assigned: DAS-68416-4 Mass Fraction 1) Certified value 2) [g/kg] Uncertainty 3) [g/kg] ERM-BF432a ERM-BF432b 5.0 0.6 ERM-BF432c 10.0 1.7 ERM-BF432d 100 13 1) Genetically modified soya event with the unique identifier DAS-68416-4. 2) Mass fraction of DAS-68416-4 soya based on the masses of genetically modified DAS-68416-4 soya seed powder and non- modified soya seed powder and their respective water content. The certified values and their uncertainties are traceable to the International System of units (SI). 3) The certified uncertainty is the expanded uncertainty with a coverage factor k = 2 corresponding to a level of confidence of about 95 % estimated in accordance with ISO/IEC Guide 98-3, Guide to the Expression of Uncertainty in Measurement (GUM:1995), ISO, 2008. How to obtain EU publications Our priced publications are available from EU Bookshop (http://bookshop.europa.eu), where you can place an order with the sales agent of your choice. The Publications Office has a worldwide network of sales agents. You can obtain their contact details by sending a fax to (352) 29 29-42758. The mission of the JRC is to provide customer-driven scientific and technical support for the conception, development, implementation and monitoring of EU policies. As a service of the European Commission, the JRC functions as a reference centre of science and technology for the Union. Close to the policy-making process, it serves the common interest of the Member States, while being independent of special interests, whether private or national. LA -N A -2505-EN -N