Studies on nerve cell affinity of biodegradable modified chitosan films

Chitosan, a natural polysaccharide that has excellent biocompatibility and biodegradability,can be used as nerve conduit material. The purpose of this work was to study the ability of chitosan and some chitosan-derived materials to facilitate nerve cell attachment, differentiation and growth. The biomaterials studied were chitosan, poly-L-lysine-blended chitosan (CP), collagen-blended chitosan (CC) and albumin-blended chitosan (CA), with collagen control material. Culture of PC12 cells and fetal mouse cerebral cortex (FMCC) cells on these biomaterials was used to evaluate their nerve cell affinity. The composite materials, including CP, CC and CA, had significantly improved nerve cell affinity compared to chitosan, as established by increasing attachment, differentiation and growth of PC12 cells. FMCC cells could also grow better on composite materials than on chitosan. CP exhibited the best nerve cell affinity among these three types of composite material. CP is an even better material in promoting neurite outgrowth than collagen, a substrate that is widely used in tissue engineering, suggesting that CP is a promising candidate material for nerve regeneration.

[1]  P. W. Kent,et al.  Fluorocarbohydrates. XXIV. Chemical and enzymic reduction and phosphorylation of 6-deoxy-6-fluoro- -D-galactose. , 1972, Carbohydrate research.

[2]  L. Chau,et al.  Surface isoelectric point of evaporated silver films: Determination by contact angle titration , 1991 .

[3]  Y Ikada,et al.  In vitro and in vivo degradation of films of chitin and its deacetylated derivatives. , 1997, Biomaterials.

[4]  P. Ducheyne,et al.  Bioactive material template for in vitro synthesis of bone. , 1995, Journal of biomedical materials research.

[5]  Y. Ikada,et al.  Cell adhesion to plasma-treated polymer surfaces , 1993 .

[6]  B. Müller,et al.  Chitosan sponges as sustained release drug carriers , 1997 .

[7]  I. Lalov,et al.  Treatment of waste water from distilleries with chitosan. , 2000 .

[8]  S. Hudson,et al.  Review of Chitin and Chitosan as Fiber and Film Formers , 1994 .

[9]  R. Muzzarelli.  Biochemical significance of exogenous chitins and chitosans in animals and patients , 1993 .

[10]  J. M. Bunn,et al.  Mechanical and barrier properties of edible chitosan films as affected by composition and storage , 1996 .

[11]  Y. Ikada,et al.  Surface modification of polymers for medical applications. , 1994, Biomaterials.

[12]  Joseph D. Andrade,et al.  Protein adsorption and materials biocompatibility: A tutorial review and suggested hypotheses , 1986 .

[13]  Z. Xiufang,et al.  Studies on nerve cell affinity of chitosan-derived materials. , 2000, Journal of biomedical materials research.

[14]  Sang Jin Lee,et al.  The effect of surface wettability on induction and growth of neurites from the PC-12 cell on a polymer surface. , 2003, Journal of colloid and interface science.

[15]  Yinye Yang,et al.  Performance Modification of Chitosan Membranes Induced by Gamma Irradiation , 2002, Journal of biomaterials applications.

[16]  R Langer,et al.  Stimulation of neurite outgrowth using an electrically conducting polymer. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[17]  O. Smidsrod,et al.  Solution properties of chitosans: conformation and chain stiffness of chitosans with different degrees of N-acetylation , 1993 .

[18]  S. Nishimura,et al.  Biological activities of biodegradable polysaccharide , 1996 .

[19]  O. Damour,et al.  Chitosan-chondroitin sulfate and chitosan-hyaluronate polyelectrolyte complexes: biological properties. , 1998, Biomaterials.

[20]  Y. Gong,et al.  Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions. , 2003, Biomaterials.

[21]  C. Schmidt,et al.  Acellular vascular tissues: natural biomaterials for tissue repair and tissue engineering. , 2000, Biomaterials.

[22]  D. Kaplan,et al.  Chitosan Film Acylation and Effects on Biodegradability , 1996 .

[23]  S T Li,et al.  A collagen‐based nerve guide conduit for peripheral nerve repair: An electrophysiological study of nerve regeneration in rodents and nonhuman primates , 1991, The Journal of comparative neurology.

[24]  A. Domard,et al.  Relation between the physicochemical characteristics of collagen and its interactions with chitosan: I. , 1993, Biomaterials.

[25]  Y. M. Elçin,et al.  Hepatocyte attachment on biodegradable modified chitosan membranes: in vitro evaluation for the development of liver organoids. , 1998, Artificial organs.

[26]  L. Greene,et al.  Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[27]  D. Barnes.  What makes nerves regenerate? , 1985, Science.

[28]  B. Dalton,et al.  Adsorption of fibronectin and vitronectin onto Primaria and tissue culture polystyrene and relationship to the mechanism of initial attachment of human vein endothelial cells and BHK-21 fibroblasts. , 1995, Biomaterials.

[29]  K. Sugioka,et al.  A Biocompatible Interface for the Geometrical Guidance of Central Neurons in Vitro , 1998 .

[30]  J. Heino.  The collagen receptor integrins have distinct ligand recognition and signaling functions. , 2000, Matrix biology : journal of the International Society for Matrix Biology.