Hadamard-transform fluorescence-lifetime imaging.

We discuss a Hadamard-transform-based fluorescence-lifetime-imaging (HT-FLI) technique for fluorescence-lifetime-imaging microscopy (FLIM). The HT-FLI uses a Fourier-transform phase-modulation fluorometer (FT-PMF) for fluorescence-lifetime measurements, where the modulation frequency of the excitation light is swept linearly in frequency from zero to a specific maximum during a fixed duration of time. Thereafter, fluorescence lifetimes are derived through Fourier transforms for the fluorescence and reference waveforms. The FT-PMF enables the analysis of multi-component samples simultaneously. HT imaging uses electronic exchange of HT illumination mask patterns, and a high-speed, high-sensitivity photomultiplier, to eliminate frame-rate issues that accompany two-dimensional image detectors.

[1]  Robert M. Clegg,et al.  Fluorescence lifetime imaging microscopy (FLIM): Spatial resolution of microstructures on the nanosecond time scale , 1993 .

[2]  Shigeo Minami,et al.  A Fluorescence Lifetime Distribution Measurement System Based on Phase-Resolved Detection Using an Image Dissector Tube , 1989 .

[3]  P. Seybold,et al.  Solvent Dependence of the Fluorescence Lifetimes of Xanthene Dyes , 1999 .

[4]  T. Iwata,et al.  Photon-counting 1.0 GHz-phase-modulation fluorometer. , 2015, The Review of scientific instruments.

[5]  Tetsuo Iwata,et al.  Construction of a Fourier-transform phase-modulation fluorometer , 2005, International Commission for Optics.

[6]  Joseph R. Lakowicz,et al.  Lifetime‐selective fluorescence imaging using an rf phase‐sensitive camera , 1991 .

[7]  David M. Coleman,et al.  A Two-Dimensional Fluorescence Lifetime Imaging System Using a Gated Image Intensifier , 1991 .

[8]  Jan Siegel,et al.  Time-domain fluorescence lifetime imaging applied to biological tissue , 2004, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

[9]  H Szmacinski,et al.  Fluorescence lifetime imaging. , 1992, Analytical biochemistry.

[10]  P. Bastiaens,et al.  Three dimensional image restoration in fluorescence lifetime imaging microscopy , 1999, Journal of microscopy.

[11]  T. Iwata,et al.  Construction of a Fourier-transform phase-modulation fluorometer , 2005 .

[12]  Horst Wallrabe,et al.  Imaging protein molecules using FRET and FLIM microscopy. , 2005, Current opinion in biotechnology.

[13]  Peter J. Verveer,et al.  Spectral Imaging in a Programmable Array Microscope by Hadamard Transform Fluorescence Spectroscopy , 1999 .

[14]  T. Lasser,et al.  Time-resolved Hadamard fluorescence imaging. , 2005, Applied optics.

[15]  J. A. Decker,et al.  Hadamard transform imager and imaging spectrometer. , 1976, Applied optics.

[16]  Gary M. Carter,et al.  Low cost phase-modulation measurements of nanosecond fluorescence lifetimes using a lock-in amplifier , 1999 .

[17]  Douglas Magde,et al.  Fluorescence Quantum Yields and Their Relation to Lifetimes of Rhodamine 6G and Fluorescein in Nine Solvents: Improved Absolute Standards for Quantum Yields¶ , 2002, Photochemistry and photobiology.

[18]  S. Ameer-Beg,et al.  Fluorescence lifetime and polarization-resolved imaging in cell biology. , 2009, Current opinion in biotechnology.