Analyses of Oxidative DNA Damage and Its Repair Activity in the Livers of 3′‐Methyl‐4‐dimethylaminoazobenzene‐treated Rodents

We measured the levels of 8‐hydroxyguanine (8‐OH‐Gua) and its repair activity in the livers of the Donryu rat, the carcinogen‐resistant DRH rat, and the ddy mouse, which were fed a 0.06% 3′‐ methyl‐4‐dimethylaminoazobenzene (3′‐MeDAB)‐containing diet. In a short‐term rat experiment (maximum 2 months), 3′‐MeDAB did not increase the 8‐OH‐Gua levels in the livers of the two rat strains, although it significantly increased the repair activity in only the Donryu rat liver at 1 and 2 months. After long‐term 3′‐MeDAB administration to the ddy mouse (8 months), the levels of 8‐OH‐Gua and its repair activity were increased in the liver by 3.6‐fold and 1.6‐fold, respectively. These experiments suggest that 3′‐MeDAB increases 8‐OH‐Gua generation in rodent liver DNA and the 8‐OH‐Gua repair assay is a reliable marker of cellular oxidative stress induced by carcinogens.

[1]  H. Itoh,et al.  Changes in levels of 8-hydroxyguanine in DNA, its repair and OGG1 mRNA in rat lungs after intratracheal administration of diesel exhaust particles. , 1999, Carcinogenesis.

[2]  T. Hirano,et al.  Increased 8‐Hydroxyguanine in DNA and Its Repair Activity in Hamster and Rat Lung after Intratracheal Instillation of Crocidolite Asbestos , 1999, Japanese journal of cancer research : Gann.

[3]  Ying Yan,et al.  Different Responses Other than the Formation of DNA‐adducts between the Livers of Carcinogen‐resistant Rats (DRH) and Carcinogen‐sensitive Rats (Donryu) to 3′‐Methyl‐4‐dimethylaminoazobenzene Administration , 1998, Japanese journal of cancer research : Gann.

[4]  C. Desmaze,et al.  Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[5]  M. Augustus,et al.  Molecular cloning and functional expression of a human cDNA encoding the antimutator enzyme 8-hydroxyguanine-DNA glycosylase. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[6]  A. Grollman,et al.  Cloning and characterization of a mammalian 8-oxoguanine DNA glycosylase. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[7]  J. Yokota,et al.  Cloning of a human homolog of the yeast OGG1 gene that is involved in the repair of oxidative DNA damage , 1997, Oncogene.

[8]  H. Aburatani,et al.  Cloning and characterization of mammalian 8-hydroxyguanine-specific DNA glycosylase/apurinic, apyrimidinic lyase, a functional mutM homologue. , 1997, Cancer research.

[9]  G. Verdine,et al.  A mammalian DNA repair enzyme that excises oxidatively damaged guanines maps to a locus frequently lost in lung cancer , 1997, Current Biology.

[10]  T. Hirano,et al.  8-hydroxyguanine levels in nuclear DNA and its repair activity in rat organs associated with age. , 1996, The journals of gerontology. Series A, Biological sciences and medical sciences.

[11]  Y. Konishi,et al.  Improved genomic/nuclear DNA extraction for 8-hydroxydeoxyguanosine analysis of small amounts of rat liver tissue. , 1995, Cancer letters.

[12]  T. Hirano,et al.  Age-associated change of 8-hydroxyguanine repair activity in cultured human fibroblasts. , 1995, Biochemical and biophysical research communications.

[13]  E. Frei,et al.  Formation and 32P-postlabeling of DNA and tRNA adducts derived from peroxidative activation of carcinogenic azo dye N,N-dimethyl-4-aminoazobenzene. , 1992, Carcinogenesis.

[14]  L. Loeb,et al.  8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G----T and A----C substitutions. , 1992, The Journal of biological chemistry.

[15]  D. Koop.  Oxidative and reductive metabolism by cytochrome P450 2E1 , 1992, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[16]  M. Fujimaki,et al.  Appearance of a Carcinoid–like Pattern in Rat Hepatic Tumors Induced by 3′–Methyl‐4–dimethyl ‐aminoazobenzene , 1991, Japanese journal of cancer research : Gann.

[17]  T. Higashi,et al.  Lipid peroxidation in the liver of carcinogen-resistant rats. , 1990, Biochimica et biophysica acta.

[18]  M. Nomura,et al.  Changes in Number of α‐Adrenergic Receptor Subtypes in Hepatocytes from Rats Fed 3′‐Methyl‐4‐dimethylaminoazobenzene , 1990 .

[19]  M. Yano,et al.  Decreased activation of carcinogens in the liver of carcinogen-resistant rats. , 1989, Cancer research.

[20]  Z. Medvedev,et al.  Age related variations of hepatocarcinogenic effect of azo dye (3′-MDAB) as linked to the level of hepatocyte polyploidization , 1988, Mechanisms of Ageing and Development.

[21]  D. Miller,et al.  Characterization and properties of the DNA adducts formed from N-methyl-4-aminoazobenzene in rats during a carcinogenic treatment regimen. , 1987, Carcinogenesis.

[22]  T. Higashi,et al.  Comparison of drug-metabolizing activities in the livers of carcinogen-sensitive parent rats and carcinogen-resistant descendants. , 1985, Cancer research.

[23]  S. Nishimura,et al.  Hydroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agents. , 1984, Nucleic acids research.

[24]  W. G. Levine,et al.  Uptake and hepatobiliary fate of two hepatocarcinogens, N,N-dimethyl-4-aminoazobenzene and 3'-methyl-N,N-dimethyl-4-aminoazobenzene, in the rat. , 1983, Cancer research.

[25]  J. Miller,et al.  Adducts from the reaction of N-benzoyloxy-N-methyl-4-aminoazobenzene with deoxyguanosine or DNA in vitro and from hepatic DNA of mice treated with N-methyl- or N,N-dimethyl-4-aminoazobenzene. , 1980, Cancer research.

[26]  G. E. Labuc,et al.  Metabolic activation of the hepatocarcinogen 3'-methyl-4-dimethylaminoazobenzene by a rat liver cell-free system. Evidence suggesting the formation of at least two electrophilic metabolites. , 1979, Biochemical pharmacology.

[27]  J. Miller,et al.  Structures of hepatic nucleic acid-bound dyes in rats given the carcinogen N-methyl-4-aminoazobenzene. , 1975, Cancer research.

[28]  J. Duchesne,et al.  Free radical evolution in rat liver following the ingestion of DAB. , 1975, Physics in medicine and biology.

[29]  J. J. Roberts,et al.  The covalent binding of metabolites of dimethylaminoazobenzene, β‐naphthylamine and aniline to nucleic acids in vivo , 1966 .

[30]  T. Nagatsu,et al.  Inhibition of hepatocellular carcinoma development and erythrocyte polyamine levels in ODS rats fed on 3'-methyl-4-dimethylaminoazobenzene by hemicalcium ascorbate, 2-O-octadecylascorbic acid, and ascorbyl palmitate. , 1996, Cancer detection and prevention.