Protective Effects of Taif Rosewater Against Testicular Impairment Induced By Lead Intoxication In Rats

This study explored treatment with Taif rosewater (RW) to protect against lead acetate‐(PbAc) induced male testicular impairment. Male Wistar rats were divided into four groups and provided drinking water containing 4% Taif RW, PbAc, 4% Taif RW followed by PbAc or normal water (controls). Serum for hormonal assays and testicular tissue for histopathological and immunohistochemical examinations and molecular study were obtained. Epididymal spermatozoa were collected for analysis. PbAc significantly reduced serum levels of follicle‐stimulating hormone (FSH), luteinising hormone (LH) and testosterone, as well as sperm count and motility percentage. It also caused a significant reduction in SOD and catalase activities, testicular CYTP450SCC, CYP17α, StAR mRNA expressions and the percentage of Bcl‐2 immunoreactivity. The percentage of caspase‐3 and NF‐ĸB immunoreactivities, as well as sperm abnormalities, was increased, as did the testicular degeneration associated with vacuolation and necrosis of spermatogenic cells. Pretreatment with Taif RW significantly reduced the negative effects of PbAc as shown by the increases in serum gonadotropins level, SOD and catalase activities, and percentage of Bcl‐2 immunoreactivity, decreases in the percentage of caspase‐3 and NF‐ĸB immunoreactivities, and improved testicular histology and sperm parameters. These data provide evidence that Taif RW protects against testicular toxicity caused by PbAc.

[1]  Sameh Saber,et al.  Vildagliptin, a DPP-4 inhibitor, attenuates carbon tetrachloride-induced liver fibrosis by targeting ERK1/2, p38α, and NF-κB signaling. , 2020, Toxicology and applied pharmacology.

[2]  E. F. Alfred,et al.  Clomiphene citrate ameliorated lead acetate-induced reproductive toxicity in male Wistar rats , 2019, JBRA assisted reproduction.

[3]  Sameh Saber,et al.  Olmesartan ameliorates chemically‐induced ulcerative colitis in rats via modulating NF&kgr;B and Nrf‐2/HO‐1 signaling crosstalk , 2019, Toxicology and applied pharmacology.

[4]  M. Soliman,et al.  Protective effects of sea cucumber (Holothuria atra) extract on testicular dysfunction induced by immune suppressant drugs in Wistar rats , 2018, Andrologia.

[5]  Santhosh Kumar,et al.  Lead Toxicity on Male Reproductive System and its Mechanism: A Review , 2018 .

[6]  Reza Sharafati Chaleshtori,et al.  Chemical Composition, Antioxidant and Antibacterial Activity of Bunium persicum, Eucalyptus globulus, and Rose Water on Multidrug-Resistant Listeria Species , 2018, Journal of evidence-based integrative medicine.

[7]  S. Nematollahi-Mahani,et al.  The effect of aqueous extract of Rosa damascena on formaldehyde-induced toxicity in mice testes , 2017, Pharmaceutical biology.

[8]  Sumeet Kour,et al.  Protective and ameliorative effect of sea buckthorn leaf extract supplementation on lead induced hemato-biochemical alterations in Wistar rats , 2016, Veterinary world.

[9]  M. Soliman,et al.  Protective effect of grape seed extract against cadmium-induced testicular dysfunction , 2016, Molecular medicine reports.

[10]  P. Patil,et al.  In vitro Antioxidant and Free Radical Scavenging Activity of Extracts of Rosa damascena Flower Petals. , 2015 .

[11]  R. A. Elgawish,et al.  Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats , 2014, Toxicology reports.

[12]  H. Sakai,et al.  Assessment of Proliferative Activity by Proliferative Cell Nuclear Antigen (PCNA) and Anti-Bromodeoxyuridine (BrdU) Immunolabeling in the Tissues of Japanese Eels (Anguilla japonica) , 2014 .

[13]  E. Abdel-Hameed,et al.  Characterization of the Phytochemical Constituents of Taif Rose and Its Antioxidant and Anticancer Activities , 2013, BioMed research international.

[14]  Ja Gilbertson,et al.  Bancroft's Theory and Practice of Histological Techniques , 2012 .

[15]  G. Türk,et al.  Rose oil inhalation protects against formaldehyde‐induced testicular damage in rats , 2012, Andrologia.

[16]  D. Naughton,et al.  Antioxidant and potential anti-inflammatory activity of extracts and formulations of white tea, rose, and witch hazel on primary human dermal fibroblast cells , 2011, Journal of Inflammation.

[17]  S. Mousavi,et al.  Study of cytotoxic properties of Rosa damascena extract in human cervix carcinoma cell line , 2011 .

[18]  Yapo Guillaume Aboua,et al.  Impact of organic hydroperoxides on rat testicular tissue and epididymal sperm , 2009 .

[19]  F. Chen,et al.  Effects of Lead Exposure on Sperm Concentrations and Testes Weight in Male Rats: A Meta-regression Analysis , 2008, Journal of toxicology and environmental health. Part A.

[20]  M. Moghimi,et al.  Evaluation of the Anticonvulsant Activities of Rosa damascena on the PTZ Induced Seizures in Wistar Rats , 2008 .

[21]  A. Songur,et al.  Protective Effects of Melatonin Against Formaldehyde-Induced Oxidative Damage and Apoptosis in Rat Testes: An Immunohistochemical and Biochemical Study , 2008, Systems biology in reproductive medicine.

[22]  K. Safranow,et al.  Increased Lipid Peroxidation and Ascorbic Acid Utilization in Testis and Epididymis of Rats Chronically Exposed to Lead , 2007, BioMetals.

[23]  Hong Tian,et al.  The protective effect of vitamin E against oxidative damage caused by formaldehyde in the testes of adult rats. , 2006, Asian journal of andrology.

[24]  G. Gonzales,et al.  Lepidium meyenii (Maca) reversed the lead acetate induced -- damage on reproductive function in male rats. , 2006, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[25]  W. Waissmann,et al.  Occupational exposure and effects on the male reproductive system. , 2006, Cadernos de saude publica.

[26]  I. Hernández-Ochoa,et al.  Low lead environmental exposure alters semen quality and sperm chromatin condensation in northern Mexico. , 2005, Reproductive toxicology.

[27]  U. R. Acharya,et al.  Protective action of vitamins on the spermatogenesis in lead-treated Swiss mice. , 2004, Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements.

[28]  D. B. Hales,et al.  Overview of steroidogenic enzymes in the pathway from cholesterol to active steroid hormones. , 2004, Endocrine reviews.

[29]  Osman Sagdic,et al.  Note: Antioxidant and Antibacterial Activities of Rosa Damascena Flower Extracts , 2004 .

[30]  E. Basım,et al.  Antibacterial activity of Rosa damascena essential oil. , 2003, Fitoterapia.

[31]  P. Foster,et al.  Quantitative changes in gene expression in fetal rat testes following exposure to di(n-butyl) phthalate. , 2003, Toxicological sciences : an official journal of the Society of Toxicology.

[32]  M. Bansal,et al.  Influence of lead and zinc on rat male reproduction at ‘biochemical and histopathological levels’ , 2001, Journal of applied toxicology : JAT.

[33]  H. Sasano,et al.  Developmental roles of the steroidogenic acute regulatory protein (StAR) as revealed by StAR knockout mice. , 2000, Molecular endocrinology.

[34]  S. Cory,et al.  The Bcl-2 protein family: arbiters of cell survival. , 1998, Science.

[35]  D. Katz,et al.  Assessment of reproductive disorders and birth defects in communities near hazardous chemical sites. III. Guidelines for field studies of male reproductive disorders. , 1997, Reproductive toxicology.

[36]  Junying Yuan,et al.  Human ICE/CED-3 Protease Nomenclature , 1996, Cell.

[37]  T. Furuchi,et al.  Inhibition of testicular germ cell apoptosis and differentiation in mice misexpressing Bcl-2 in spermatogonia. , 1996, Development.

[38]  W. Abdo,et al.  Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats , 2017 .

[39]  H. Jahromi,et al.  Effect of Damask Rose Extract on FSH, LH and Testosterone Hormones in Rats , 2016 .

[40]  E. Abdel-Hameed,et al.  Total phenolic, in vitro antioxidant activity and safety assessment (Acute, sub-chronic and chronic toxicity) of industrial Taif rose waterby-product in mice , 2015 .

[41]  S. Al-Masri.  Effect of pumpkin oil and vitamin E on lead induced testicular toxicity in male rats. , 2015 .

[42]  Zahra Memariani,et al.  Comparative analysis of phenolic compounds in two samples of Rosa damascena by HPLC. , 2015 .

[43]  H. Baydar,et al.  Phenolic compounds, antiradical activity and antioxidant capacity of oil-bearing rose (Rosa damascena Mill.) extracts , 2013 .

[44]  S. Himesh,et al.  RADICAL SCAVENGING ACTIVITIES AND NATURAL INDICATOR ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACT OF Rosa Damascena , 2012 .

[45]  B. Zhivotovsky,et al.  Cell death mechanisms and their implications in toxicology. , 2011, Toxicological sciences : an official journal of the Society of Toxicology.

[46]  H. Rakhshandeh,et al.  ANTINOCICEPTIVE EFFECT OF ROSA DAMASCENA IN MICE , 2008 .

[47]  H. Pahl,et al.  Activators and target genes of Rel/NF-kappaB transcription factors. , 1999, Oncogene.

[48]  M. Bansal,et al.  Cell kinetics of the rat seminiferous epithelium following lead acetate treatment , 1996 .

[49]  A. Baldwin,et al.  The NF-kappa B and I kappa B proteins: new discoveries and insights. , 1996, Annual review of immunology.

[50]  L. Russell,et al.  Histological and Histopathological Evaluation of the Testis , 1990 .