论文信息 - Enzymatic catalysis in organic media at 100 degrees C.

Enzymatic catalysis in organic media at 100 degrees C.

Porcine pancreatic lipase catalyzes the transesterification reaction between tributyrin and various primary and secondary alcohols in a 99 percent organic medium. Upon further dehydration, the enzyme becomes extremely thermostable. Not only can the dry lipase withstand heating at 100 degrees C for many hours, but it exhibits a high catalytic activity at that temperature. Reduction in water content also alters the substrate specificity of the lipase: in contrast to its wet counterpart, the dry enzyme does not react with bulky tertiary alcohols.

A. Klibanov | A M Klibanov | A Zaks | A. Zaks

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