Improvement of glucose production by raw starch degradingenzyme utilizing acid-treated sago starch as substrate

The native sago starch exists as a compact crystalline structure and is not efficiently hydrolyzed by Raw Starch Degrading Enzyme (RSDE). In order to enhance its hydrolysability, the starch was treated with acid and heated below its gelatinization temperature, thus increasing the accessibility of the sago starch granule to enzymatic attack. Results showed that treatment of sago starch with acid at pH 2.0 and temperature 65 o C for 2 hours greatly enhanced its conversion rate to glucose from 53.3% to 71.9%. It is clearly shown that high yield of glucose is produced during hydrolysis of acid-treated sago starch using the Raw Starch Degrading Enzyme from Acremonium sp. The difference between the acid-treated and untreated sago starch in this study could be due to the differences on the surface of the sago starch granule which may influence the accessibility and diffusion of enzyme into the starch during hydrolysis.

[1]  N. Saari,et al.  Purification and characterization of sago starch-degrading glucoamylase from Acremonium sp. endophytic fungus , 2000 .

[2]  Hassan,et al.  Improvement in raw sago starch degrading enzyme production from Acremonium sp. endophytic fungus using carbon and nitrogen sources. , 2000, Enzyme and microbial technology.

[3]  N. Saari,et al.  Raw starch-degrading enzyme from newly isolated strains of endophytic fungi , 2000 .

[4]  E. Pérez,et al.  ariations in the Gelatinization Profiles of Cassava, Sagu and Arrowroot Native Starches as Measured with Different Thermal and Mechanical Methods , 1998 .

[5]  C. Oates,et al.  Effect of annealing on the hydrolysis of sago starch granules , 1997 .

[6]  J. Delcour,et al.  Effects of hydrothermal treatments on the rheological properties of potato starch , 1997 .

[7]  C. Mohácsi-Farkas,et al.  Application of differential scanning calorimetry in food research and food quality assurance , 1996 .

[8]  C. Oates,et al.  Sago starch as a biomass source : Raw sago starch hydrolysis by commercial enzymes , 1996 .

[9]  O. Campanella,et al.  Influence of extrusion variables on subsequent saccharification behaviour of sago starch , 1995 .

[10]  W. Wang,et al.  Pattern of enzyme hydrolysis in raw sago starch: effects of processing history , 1995 .

[11]  R. Hoover,et al.  Effect of heat-moisture treatment on the structure and physicochemical properties of cereal, legume, and tuber starches. , 1994, Carbohydrate research.

[12]  Antonio M. Martin,et al.  ISOLATION AND CHARACTERIZATION OF LIMA BEAN (PHASEOLUS LUNATUS) STARCH , 1991 .

[13]  J. Brooks,et al.  Production and Size Distribution of Rice Maitodextrins Hydrolyzed from Milled Rice Flour using Heat‐Stable Alpha‐Amylase , 1989 .

[14]  Y. Kamagata,et al.  Production of a Raw Starch Saccharifying Enzyme by Corticium rolfsii , 1986 .

[15]  B. O. Juliano,et al.  Physicochemical Properties of Starch of Intermediate‐Amylose and Waxy Rices Differing in Grain Quality , 1981 .

[16]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .