Cellulose conjugated FITC-labelled mesoporous silica nanoparticles: intracellular accumulation and stimuli responsive doxorubicin release.

Herein, we design novel cellulose conjugated mesoporous silica nanoparticle (CLS-MSP) based nanotherapeutics for stimuli responsive intracellular doxorubicin (DOX) delivery. DOX molecules are entrapped in pores of the fabricated mesoporous silica nanoparticles (MSPs) while cellulose is used as an encapsulating material through esterification on the outlet of the pores of the MSPs to avoid premature DOX release under physiological conditions. In in vitro studies, stimuli responsive DOX release is successfully achieved from DOX loaded cellulose conjugated mesoporous silica nanoparticles (DOX/CLS-MSPs) by pH and cellulase triggers. Intracellular accumulation of DOX/CLS-MSPs in human liver cancer cells (HepG2 cells) is investigated through confocal microscope magnification. Cell viability of HepG2 cells is determined as the percentage of the cells incubated with DOX/CLS-MSPs compared with that of non-incubated cells through an MTT assay.

[1]  Nannan Liu,et al.  Real-time monitoring of enzyme-free strand displacement cascades by colorimetric assays. , 2015, Nanoscale.

[2]  Ruixue Duan,et al.  Polar organic solvents accelerate the rate of DNA strand replacement reaction. , 2015, The Analyst.

[3]  Yongmei Jia,et al.  Dual stimuli-responsive nano-vehicles for controlled drug delivery: mesoporous silica nanoparticles end-capped with natural chitosan. , 2014, Chemical communications.

[4]  Liang Cheng,et al.  Functional nanomaterials for phototherapies of cancer. , 2014, Chemical reviews.

[5]  Tianyue Jiang,et al.  Folding graft copolymer with pendant drug segments for co-delivery of anticancer drugs. , 2014, Biomaterials.

[6]  Q. Ping,et al.  Sequential intra-intercellular nanoparticle delivery system for deep tumor penetration. , 2014, Angewandte Chemie.

[7]  Zhimin Tao.  Mesoporous silica-based nanodevices for biological applications , 2014 .

[8]  X. Yang,et al.  Natural gelatin capped mesoporous silica nanoparticles for intracellular acid-triggered drug delivery. , 2013, Langmuir : the ACS journal of surfaces and colloids.

[9]  Neil R. Cameron,et al.  ‘Multicopy Multivalent’ Glycopolymer-Stabilized Gold Nanoparticles as Potential Synthetic Cancer Vaccines , 2013, Journal of the American Chemical Society.

[10]  Eric C. Carnes,et al.  Mesoporous silica nanoparticle nanocarriers: biofunctionality and biocompatibility. , 2013, Accounts of chemical research.

[11]  Xiaojun Cai,et al.  Mesoporous silica nanoparticles capped with disulfide-linked PEG gatekeepers for glutathione-mediated controlled release. , 2012, ACS applied materials & interfaces.

[12]  Jun Lin,et al.  Functionalized mesoporous silica materials for controlled drug delivery. , 2012, Chemical Society reviews.

[13]  Yu Chen,et al.  Nuclear-targeted drug delivery of TAT peptide-conjugated monodisperse mesoporous silica nanoparticles. , 2012, Journal of the American Chemical Society.

[14]  Yaping Li,et al.  Controlled intracellular release of doxorubicin in multidrug-resistant cancer cells by tuning the shell-pore sizes of mesoporous silica nanoparticles. , 2011, ACS nano.

[15]  Michael J. Sailor,et al.  Bioresponsive Mesoporous Silica Nanoparticles for Triggered Drug Release , 2011, Journal of the American Chemical Society.

[16]  C. Marquis,et al.  Acid Degradable and Biocompatible Polymeric Nanoparticles for the Potential Codelivery of Therapeutic Agents , 2011 .

[17]  Xiaoyuan Chen,et al.  HSA coated iron oxide nanoparticles as drug delivery vehicles for cancer therapy. , 2011, Molecular pharmaceutics.

[18]  Kostas Kostarelos,et al.  Liposomes: from a clinically established drug delivery system to a nanoparticle platform for theranostic nanomedicine. , 2011, Accounts of chemical research.

[19]  Courtney R. Thomas,et al.  Mechanized silica nanoparticles: a new frontier in theranostic nanomedicine. , 2011, Accounts of chemical research.

[20]  Tingting Wang,et al.  Multifunctional hollow mesoporous silica nanocages for cancer cell detection and the combined chemotherapy and photodynamic therapy. , 2011, ACS applied materials & interfaces.

[21]  G. Zhu,et al.  pH-Triggered controlled drug release from mesoporous silica nanoparticles via intracelluar dissolution of ZnO nanolids. , 2011, Journal of the American Chemical Society.

[22]  Hua Ai,et al.  Surface-engineered magnetic nanoparticle platforms for cancer imaging and therapy. , 2011, Accounts of chemical research.

[23]  Li Yuan,et al.  Preparation of pH-Responsive Mesoporous Silica Nanoparticles and Their Application in Controlled Drug Delivery , 2011 .

[24]  Jianlin Shi,et al.  Mesoporous silica nanoparticle based nano drug delivery systems: synthesis, controlled drug release and delivery, pharmacokinetics and biocompatibility , 2011 .

[25]  Fang Pu,et al.  Polyvalent nucleic acid/mesoporous silica nanoparticle conjugates: dual stimuli-responsive vehicles for intracellular drug delivery. , 2011, Angewandte Chemie.

[26]  S. Gruner,et al.  Highly aminated mesoporous silica nanoparticles with cubic pore structure. , 2011, Journal of the American Chemical Society.

[27]  P. Liu,et al.  Mesoporous silica nanoparticles end-capped with collagen: redox-responsive nanoreservoirs for targeted drug delivery. , 2011, Angewandte Chemie.

[28]  Chun-hua Lu,et al.  Bioresponsive controlled release using mesoporous silica nanoparticles capped with aptamer-based molecular gate. , 2011, Journal of the American Chemical Society.

[29]  K. Akiyoshi,et al.  Nanogel engineering for new nanobiomaterials: from chaperoning engineering to biomedical applications. , 2010, Chemical record.

[30]  Jiwei Cui,et al.  Encapsulation of Water‐Insoluble Drugs in Polymer Capsules Prepared Using Mesoporous Silica Templates for Intracellular Drug Delivery , 2010, Advanced materials.

[31]  J. F. Stoddart,et al.  Autonomous in vitro anticancer drug release from mesoporous silica nanoparticles by pH-sensitive nanovalves. , 2010, Journal of the American Chemical Society.

[32]  Zongxi Li,et al.  Biocompatibility, biodistribution, and drug-delivery efficiency of mesoporous silica nanoparticles for cancer therapy in animals. , 2010, Small.

[33]  J. Zink,et al.  Engineered design of mesoporous silica nanoparticles to deliver doxorubicin and P-glycoprotein siRNA to overcome drug resistance in a cancer cell line. , 2010, ACS nano.

[34]  J. Ho,et al.  Biofunctionalized phospholipid-capped mesoporous silica nanoshuttles for targeted drug delivery: improved water suspensibility and decreased nonspecific protein binding. , 2010, ACS nano.

[35]  Maohong Fan,et al.  Prodrugs forming high drug loading multifunctional nanocapsules for intracellular cancer drug delivery. , 2010, Journal of the American Chemical Society.

[36]  S. Choi,et al.  Uniform mesoporous dye-doped silica nanoparticles decorated with multiple magnetite nanocrystals for simultaneous enhanced magnetic resonance imaging, fluorescence imaging, and drug delivery. , 2010, Journal of the American Chemical Society.

[37]  Marilena Loizidou,et al.  Liposomes and nanoparticles: nanosized vehicles for drug delivery in cancer. , 2009, Trends in pharmacological sciences.

[38]  J. F. Stoddart,et al.  Controlled-access hollow mechanized silica nanocontainers. , 2009, Journal of the American Chemical Society.

[39]  Yuen A. Lau,et al.  Mechanised nanoparticles for drug delivery. , 2009, Nanoscale.

[40]  R. Martínez‐Máñez,et al.  Controlled delivery systems using antibody-capped mesoporous nanocontainers. , 2009, Journal of the American Chemical Society.

[41]  C. Haynes,et al.  Synthesis and Characterization of Biocompatible and Size-Tunable Multifunctional Porous Silica Nanoparticles , 2009 .

[42]  J. Michaelis,et al.  Nanostructured silica materials as drug-delivery systems for Doxorubicin: single molecule and cellular studies. , 2009, Nano letters.

[43]  V. S. Lin,et al.  Mesoporous silica nanoparticle-based double drug delivery system for glucose-responsive controlled release of insulin and cyclic AMP. , 2009, Journal of the American Chemical Society.

[44]  Juan L. Vivero-Escoto,et al.  Photoinduced intracellular controlled release drug delivery in human cells by gold-capped mesoporous silica nanosphere. , 2009, Journal of the American Chemical Society.

[45]  B. Rutt,et al.  Enhanced cell uptake of superparamagnetic iron oxide nanoparticles functionalized with dendritic guanidines. , 2008, Bioconjugate chemistry.

[46]  Robert Langer,et al.  Targeted delivery of cisplatin to prostate cancer cells by aptamer functionalized Pt(IV) prodrug-PLGA–PEG nanoparticles , 2008, Proceedings of the National Academy of Sciences.

[47]  Wenbin Lin,et al.  Nanoscale coordination polymers for platinum-based anticancer drug delivery. , 2008, Journal of the American Chemical Society.

[48]  Monty Liong,et al.  Multifunctional inorganic nanoparticles for imaging, targeting, and drug delivery. , 2008, ACS nano.

[49]  S. Nie,et al.  Therapeutic Nanoparticles for Drug Delivery in Cancer , 2008, Clinical Cancer Research.

[50]  Byeong-Su Kim,et al.  Hydrogen-bonding layer-by-layer-assembled biodegradable polymeric micelles as drug delivery vehicles from surfaces. , 2008, ACS nano.

[51]  Monty Liong,et al.  Mesoporous silica nanoparticles as a delivery system for hydrophobic anticancer drugs. , 2007, Small.

[52]  Hung-Ting Chen,et al.  Synthesis and functionalization of a mesoporous silica nanoparticle based on the sol-gel process and applications in controlled release. , 2007, Accounts of chemical research.

[53]  Sang Cheon Lee,et al.  Controlled release of guest molecules from mesoporous silica particles based on a pH-responsive polypseudorotaxane motif. , 2007, Angewandte Chemie.

[54]  D. Schmaljohann.  Thermo- and pH-responsive polymers in drug delivery. , 2006, Advanced drug delivery reviews.

[55]  Jia Guo,et al.  Poly(N-isopropylacrylamide)-Coated Luminescent/Magnetic Silica Microspheres: Preparation, Characterization, and Biomedical Applications , 2006 .

[56]  María Vallet-Regí,et al.  Functionalization of mesoporous materials with long alkyl chains as a strategy for controlling drug delivery pattern , 2006 .

[57]  Giulio F. Paciotti,et al.  Colloidal gold nanoparticles: a novel nanoparticle platform for developing multifunctional tumor‐targeted drug delivery vectors , 2006 .

[58]  M. Ferrari.  Cancer nanotechnology: opportunities and challenges , 2005, Nature Reviews Cancer.

[59]  A. Kassner,et al.  Molecular Imaging of Angiogenesis in Nascent Vx-2 Rabbit Tumors Using a Novel ανβ3-targeted Nanoparticle and 1.5 Tesla Magnetic Resonance Imaging , 2003 .

[60]  Victor S-Y Lin,et al.  A mesoporous silica nanosphere-based carrier system with chemically removable CdS nanoparticle caps for stimuli-responsive controlled release of neurotransmitters and drug molecules. , 2003, Journal of the American Chemical Society.

[61]  P. Gupta,et al.  Hydrogels: from controlled release to pH-responsive drug delivery. , 2002, Drug discovery today.

[62]  G. Evan,et al.  Proliferation, cell cycle and apoptosis in cancer , 2001, Nature.

[63]  E. Tahara,et al.  Scirrhous argyrophil cell carcinoma of the stomach with multiple production of polypeptide hormones, amine, CEA, lysozyme, and HCG , 1982, Cancer.

[64]  M. Gottesman.  Mechanisms of cancer drug resistance. , 2002, Annual review of medicine.