Isolation and molecular typing of Cronobacter spp. in commercial powdered infant formula and follow-up formula.

Cronobacter spp. (Enterobacter sakazakii) are important foodborne pathogens. Infections with this pathogen can lead to neonatal meningitis, necrotizing enterocolitis, and bacteremia. This study examined Cronobacter spp. contamination in commercial powdered infant formulas (PIFs) and follow-up formulas (FUFs) in China. Forty-nine of 399 samples were contaminated with Cronobacter spp. and 10.2% of the isolates were resistant to cefotaxime; in contrast, all of the tested isolates were susceptible to amikacin, amoxicillin/clavulanic acid, cefepime, ciprofloxacin, imipenem, and meropenem. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) analyses produced a total of 16 PFGE banding patterns and 11 sequence types (STs), including 7 novel STs. In summary, the rates at which Cronobacter spp. were isolated from commercial PIF and FUF samples in China were relatively high, and the isolated strains exhibited high susceptibility in vitro to most antibiotics. The PFGE method exhibited higher typing capability than the MLST method, and molecular typing results revealed that the contamination of PIF and FUF with Cronobacter spp. in China may be mainly due to the addition of contaminated materials. Thus, the development of more effective control strategies during the manufacturing process is needed.

[1]  S. Forsythe,et al.  Cronobacter sakazakii ST4 Strains and Neonatal Meningitis, United States , 2013, Emerging infectious diseases.

[2]  S. Forsythe,et al.  Microbiological assessment and evaluation of rehydration instructions on powdered infant formulas, follow-up formulas, and infant foods in Malaysia. , 2013, Journal of dairy science.

[3]  N. Binsztein,et al.  Development and validation of a PulseNet standardized protocol for subtyping isolates of Cronobacter species. , 2012, Foodborne pathogens and disease.

[4]  P. Desai,et al.  Diversity of the Cronobacter Genus as Revealed by Multilocus Sequence Typing , 2012, Journal of Clinical Microbiology.

[5]  R. P. Ross,et al.  Cronobacter spp. in powdered infant formula. , 2012, Journal of food protection.

[6]  M. Rhee,et al.  Microbial contamination of food products consumed by infants and babies in Korea , 2011, Letters in applied microbiology.

[7]  K. Nahar,et al.  Isolation and molecular identification of Cronobacter spp. from powdered infant formula (PIF) in Bangladesh. , 2010, International journal of food microbiology.

[8]  P. Whyte,et al.  Cronobacter (Enterobacter sakazakii): an opportunistic foodborne pathogen. , 2010, Foodborne pathogens and disease.

[9]  R. Leggiadro.  Cronobacter species isolation in two infants - New Mexico, 2008. , 2010, MMWR. Morbidity and mortality weekly report.

[10]  S. Forsythe,et al.  International survey of Cronobacter sakazakii and other Cronobacter spp. in follow up formulas and infant foods. , 2009, International journal of food microbiology.

[11]  G. Duffy,et al.  Surveillance and characterisation by pulsed-field gel electrophoresis of Cronobacter spp. in farming and domestic environments, food production animals and retail foods. , 2009, International journal of food microbiology.

[12]  F. Pagotto,et al.  Cronobacter spp. (Enterobacter sakazakii): advice, policy and research in Canada. , 2009, International journal of food microbiology.

[13]  N. Binsztein,et al.  Characterization and subtyping of Cronobacter spp. from imported powdered infant formulae in Argentina. , 2009, International journal of food microbiology.

[14]  M. Friedemann.  Epidemiology of invasive neonatal Cronobacter (Enterobacter sakazakii) infections , 2009, European Journal of Clinical Microbiology & Infectious Diseases.

[15]  S. Forsythe,et al.  Survival and growth of Cronobacter species (Enterobacter sakazakii) in wheat‐based infant follow‐on formulas , 2009, Letters in applied microbiology.

[16]  S. Heu,et al.  Prevalence and genetic diversity of Enterobacter sakazakii in ingredients of infant foods. , 2008, International journal of food microbiology.

[17]  T. Osaili,et al.  Isolation of Enterobacter sakazakii and other Enterobacter sp. from food and food production environments , 2007 .

[18]  M. Friedemann.  Enterobacter sakazakii in food and beverages (other than infant formula and milk powder). , 2007, International journal of food microbiology.

[19]  P. Whyte,et al.  Application of pulsed-field gel electrophoresis to characterise and trace the prevalence of Enterobacter sakazakii in an infant formula processing facility. , 2007, International journal of food microbiology.

[20]  E. Usleber,et al.  Enterobacteriaceae in dehydrated powdered infant formula manufactured in Indonesia and Malaysia. , 2006, Journal of food protection.

[21]  C. Braden,et al.  Invasive Enterobacter sakazakii Disease in Infants , 2006, Emerging infectious diseases.

[22]  S. Forsythe,et al.  Isolation of Enterobacter sakazakii and other Enterobacteriaceae from powdered infant formula milk and related products , 2004 .

[23]  J. Farber.  Enterobacter sakazakii—new foods for thought? , 2004, The Lancet.

[24]  K. K. Lai.  Enterobacter sakazakii Infections among Neonates, Infants, Children, and Adults: Case Reports and a Review of the Literature , 2001, Medicine.

[25]  C. Block,et al.  Enterobacter sakazakii infection in the newborn , 2001, Acta paediatrica.

[26]  S. Lauwers,et al.  Outbreak of Necrotizing Enterocolitis Associated with Enterobacter sakazakii in Powdered Milk Formula , 2001, Journal of Clinical Microbiology.

[27]  J. Farber,et al.  Incidence, Survival, and Growth of Enterobacter sakazakii in Infant Formula. , 1997, Journal of food protection.

[28]  H L Muytjens,et al.  Quality of powdered substitutes for breast milk with regard to members of the family Enterobacteriaceae , 1988, Journal of clinical microbiology.

[29]  S. Hewitt,et al.  1987 , 1987, Literatur in der SBZ/DDR.

[30]  A. M. Urmenyi,et al.  Neonatal death from pigmented coliform infection. , 1961, Lancet.