The Plant Dehydrins: Structure and Putative Functions

This review deals with recent data on the structure and biochemical properties of dehydrins, proteins that are normally synthesized in maturating seeds during their desiccation, and also in vegetative tissues of plants treated with abscisic acid or exposed to environmental stress factors that result in cellular dehydration. The dehydrins are considered as stress proteins involved in formation of plant protective reactions against dehydration. The generally accepted classification of dehydrins is based on their structural features, such as the presence of conserved sequences, designated as Y-, S-, and K-segments. The K-segment representing a highly conserved 15 amino acid motif (EKKGIMDKIKEKLPG) forming amphiphilic α-helix has been found in all dehydrins. The pathways of regulation of dehydrin gene expression, putative functions of dehydrins, and molecular mechanisms of their actions are discussed.

[1]  T. Close,et al.  Purification and partial characterization of a dehydrin involved in chilling tolerance during seedling emergence of cowpea. , 1999, Plant physiology.

[2]  M. Thomashow.  Role of cold-responsive genes in plant freezing tolerance. , 1998, Plant physiology.

[3]  L. Dure,et al.  Sequence and characterization of 6 Lea proteins and their genes from cotton , 1988, Plant Molecular Biology.

[4]  T. Close,et al.  Dehydrins: genes, proteins, and associations with phenotypic traits , 1997 .

[5]  K. Yamaguchi-Shinozaki,et al.  An Arabidopsis gene family encoding DRE/CRT binding proteins involved in low-temperature-responsive gene expression. , 1998, Biochemical and biophysical research communications.

[6]  K. Shinozaki,et al.  Gene Expression and Signal Transduction in Water-Stress Response , 1997, Plant physiology.

[7]  Stephen M. Mount,et al.  The genome sequence of Drosophila melanogaster. , 2000, Science.

[8]  T. Close,et al.  Expression of the barley dehydrin multigene family and the development of freezing tolerance , 2000, Molecular and General Genetics MGG.

[9]  T. Moritz,et al.  Independent Activation of Cold Acclimation by Low Temperature and Short Photoperiod in Hybrid Aspen1 , 2002, Plant Physiology.

[10]  L. Franco,et al.  A chromatin-associated protein from pea seeds preferentially binds histones H3 and H4. , 2002, European journal of biochemistry.

[11]  M. Van Montagu,et al.  Molecular and Physiological Responses to Abscisic Acid and Salts in Roots of Salt-Sensitive and Salt-Tolerant Indica Rice Varieties , 1995, Plant physiology.

[12]  C. Rock,et al.  Tansley Review No. 120: Pathways to abscisic acid-regulated gene expression. , 2000, The New phytologist.

[13]  H. Tuominen,et al.  Seasonal changes in bud dormancy in relation to bud morphology, water and starch content, and abscisic acid concentration in adult trees of Betula pubescens. , 1994, Tree physiology.

[14]  P. Mäkelä,et al.  Ectopic expression of ABI3 gene enhances freezing tolerance in response to abscisic acid and low temperature in Arabidopsis thaliana. , 2008, The Plant journal : for cell and molecular biology.

[15]  M. Mar Albà,et al.  The maize abscisic acid-responsive protein Rab17 is located in the nucleus and interacts with nuclear localization signals. , 1994, The Plant cell.

[16]  R. Finkelstein,et al.  Abscisic Acid Signaling in Seeds and Seedlings Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010441. , 2002, The Plant Cell Online.

[17]  M. Wisniewski,et al.  Cold Acclimation in Genetically Related (Sibling) Deciduous and Evergreen Peach (Prunus persica [L.] Batsch) (II. A 60-Kilodalton Bark Protein in Cold-Acclimated Tissues of Peach Is Heat Stable and Related to the Dehydrin Family of Proteins) , 1994, Plant physiology.

[18]  D. Bartels,et al.  Drought- and desiccation-induced modulation of gene expression in plants. , 2002, Plant, cell & environment.

[19]  J. Mullet,et al.  Modulation of Dehydration Tolerance in Soybean Seedlings (Dehydrin Mat1 Is Induced by Dehydration but Not by Abscisic Acid) , 1997, Plant physiology.

[20]  K. Shinozaki,et al.  cDNA cloning of ECP40, an embryogenic-cell protein in carrot, and its expression during somatic and zygotic embryogenesis , 1993, Plant Molecular Biology.

[21]  Håkan Wennerström,et al.  Role of hydration and water structure in biological and colloidal interactions , 1996, Nature.

[22]  K. Taulavuori,et al.  Soluble proteins and dehydrins in nitrogen-fertilized Scots pine seedlings during deacclimation and the onset of growth , 2000 .

[23]  P. Busk,et al.  Regulation of abscisic acid-induced transcription , 1998, Plant Molecular Biology.

[24]  R. Quatrano,et al.  Common amino acid sequence domains among the LEA proteins of higher plants , 1989, Plant Molecular Biology.

[25]  Bingru Huang,et al.  Protein Alterations in Tall Fescue in Response to Drought Stress and Abscisic Acid. , 2002, Crop science.

[26]  S. Uknes,et al.  Cloning and characterization of a cDNA encoding a mRNA rapidly-induced by ABA in barley aleurone layers , 2004, Plant Molecular Biology.

[27]  R. Arora,et al.  Chill‐responsive dehydrins in blueberry: Are they associated with cold hardiness or dormancy transitions? , 1997 .

[28]  M. Robertson,et al.  Allelic variation in the dehydrin gene family of ‘Himalaya’ barley (Hordeum vulgare L.) , 1998, Theoretical and Applied Genetics.

[29]  F. Tremblay,et al.  Induction of tolerance to fast desiccation in black spruce (Picea mariana) somatic embryos: relationship between partial water loss, sugars, and dehydrins. , 2002, Physiologia plantarum.

[30]  O. Schabenberger,et al.  Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. , 1998, Science.

[31]  T. Kazuoka,et al.  Purification and Characterization of COR85-Oligomeric Complex from Cold-Acclimated Spinach , 1994 .

[32]  T. Artlip,et al.  Seasonal expression of a dehydrin gene in sibling deciduous and evergreen genotypes of peach (Prunus persica [L.] Batsch) , 2004, Plant Molecular Biology.

[33]  J. Svensson,et al.  Stress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana , 2001, Plant Molecular Biology.

[34]  R. Arora,et al.  A 25-kDa dehydrin associated with genotype- and age-dependent leaf freezing-tolerance in Rhododendron: a genetic marker for cold hardiness? , 1999, Theoretical and Applied Genetics.

[35]  T. Close,et al.  Temporal accumulation and ultrastructural localization of dehydrins in Zea mays , 1997 .

[36]  L. Jouanin,et al.  Isolation and characterization of a dehydrin gene from white spruce induced upon wounding, drought and cold stresses , 2000, Plant Molecular Biology.

[37]  A. Wood,et al.  Characterization and expression of dehydrins in water‐stressed Sorghum bicolor , 1997 .

[38]  E. Bray.  Molecular Responses to Water Deficit , 1993, Plant physiology.

[39]  F. Neidhardt,et al.  Escherichia Coli and Salmonella: Typhimurium Cellular and Molecular Biology , 1987 .

[40]  T. Close,et al.  Barley Cbf3 Gene Identification, Expression Pattern, and Map Location1 , 2002, Plant Physiology.

[41]  E. Rassart,et al.  Cloning, characterization, and expression of a cDNA encoding a 50-kilodalton protein specifically induced by cold acclimation in wheat. , 1992, Plant physiology.

[42]  G. Galau,et al.  Developmental biochemistry of cottonseed embryogenesis and germination: changing messenger ribonucleic acid populations as shown by in vitro and in vivo protein synthesis. , 1981, Biochemistry.

[43]  M. Robertson,et al.  Sequence analysis and hormonal regulation of a dehydrin promoter from barley, Hordeum vulgare , 1995 .

[44]  T. Close,et al.  The barley (Hordeum vulgare L.) dehydrin multigene family: sequences, allele types, chromosome assignments, and expression characteristics of 11 Dhn genes of cv Dicktoo , 1999, Theoretical and Applied Genetics.

[45]  A. Levi,et al.  Complementary DNA cloning, sequencing and expression of an unusual dehydrin from blueberry floral buds , 1999 .

[46]  Jian-Kang Zhu,et al.  Salt and drought stress signal transduction in plants. , 2002, Annual review of plant biology.

[47]  T. Close,et al.  Nucleotide Sequence of a Gene Encoding a 58.5-Kilodalton Barley Dehydrin That Lacks a Serine Tract , 1995, Plant physiology.

[48]  F. Sarhan,et al.  The wheat wcs120 gene family. A useful model to understand the molecular genetics of freezing tolerance in cereals , 1997 .

[49]  T. Close,et al.  A newly identified barley gene, Dhn12, encoding a YSK2 DHN, is located on chromosome 6H and has embryo-specific expression , 2000, Theoretical and Applied Genetics.

[50]  Jean Danyluk,et al.  Accumulation of an Acidic Dehydrin in the Vicinity of the Plasma Membrane during Cold Acclimation of Wheat , 1998, Plant Cell.

[51]  T. Close,et al.  Purification, immunolocalization, cryoprotective, and antifreeze activity of PCA60: A dehydrin from peach (Prunus persica) , 1999 .

[52]  T. Close.  Dehydrins: A commonalty in the response of plants to dehydration and low temperature , 1997 .

[53]  T. Close,et al.  A ca. 40 kDa maize (Zea mays L.) embryo dehydrin is encoded by the dhn2 locus on chromosome 9 , 1998, Plant Molecular Biology.

[54]  W. Hartung,et al.  Abscisic acid and the induction of desiccation tolerance in the extremely xerophilic liverwort Exormotheca holstii , 1994, Planta.

[55]  T. Close,et al.  A view of plant dehydrins using antibodies specific to the carboxy terminal peptide , 1993, Plant Molecular Biology.

[56]  P. Chandler,et al.  Pea dehydrins: identification, characterisation and expression , 1992, Plant Molecular Biology.

[57]  L. Franco,et al.  A pea nuclear protein that is induced by dehydration belongs to the vicilin superfamily. , 2000, European journal of biochemistry.

[58]  E. Stockinger,et al.  Role of the Arabidopsis CBF transcriptional activators in cold acclimation , 2001 .

[59]  D. Gonzalez,et al.  Hahb‐4, a homeobox‐leucine zipper gene potentially involved in abscisic acid‐dependent responses to water stress in sunflower , 2002 .

[60]  A. Jensen,et al.  Phosphorylation mediates the nuclear targeting of the maize Rab17 protein. , 1998, The Plant journal : for cell and molecular biology.

[61]  G. Galau,et al.  Developmental biochemistry of cottonseed embryogenesis and germination: changing messenger ribonucleic acid populations as shown by reciprocal heterologous complementary deoxyribonucleic acid--messenger ribonucleic acid hybridization. , 1981, Biochemistry.

[62]  S. Torres-Schumann,et al.  Expression, tissue distribution and subcellular localization of dehydrin TAS14 in salt-stressed tomato plants , 1994, Plant Molecular Biology.

[63]  K. Shinozaki,et al.  Two Transcription Factors, DREB1 and DREB2, with an EREBP/AP2 DNA Binding Domain Separate Two Cellular Signal Transduction Pathways in Drought- and Low-Temperature-Responsive Gene Expression, Respectively, in Arabidopsis , 1998, Plant Cell.

[64]  P. Lammers,et al.  An Osmotic Stress Protein of Cyanobacteria Is Immunologically Related to Plant Dehydrins , 1993, Plant physiology.

[65]  F. Parcy,et al.  Interactions between the ABI1 and the ectopically expressed ABI3 genes in controlling abscisic acid responses in Arabidopsis vegetative tissues. , 1997, The Plant journal : for cell and molecular biology.

[66]  C. Vitagliano,et al.  Expression of a dehydrin gene during embryo development and drought stress in ABA-deficient mutants of sunflower (Helianthus annuus L.) , 1999, Plant Molecular Biology.

[67]  R. W. Davis,et al.  Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis. , 1998, Science.

[68]  D. McCarty,et al.  The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator , 1991, Cell.

[69]  F. Cellier,et al.  Molecular and physiological responses to water deficit in drought-tolerant and drought-sensitive lines of sunflower. Accumulation of dehydrin transcripts correlates with tolerance. , 1998, Plant physiology.

[70]  R. Quatrano,et al.  The nucleotide sequence of a cDNA clone encoding the wheat Em protein. , 1987, Nucleic acids research.

[71]  T. Close,et al.  A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn , 1989, Plant Molecular Biology.

[72]  P. Rinne,et al.  Dehydrins in cold-acclimated apices of birch (Betula pubescens Ehrh.): production, localization and potential role in rescuing enzyme function during dehydration , 1999, Planta.

[73]  T. Close,et al.  PROTEINS IMMUNOLOGICALLY RELATED TO DEHYDRINS IN FUCOID ALGAE , 1998 .