Biomedical imaging by means of linear and non-linear Raman microspectroscopy

Biomedical imaging by means of nonlinear Raman microspectroscopy offers novel approaches towards individualized decisions on therapy routines as diagnosis is possible on the chemical composition of tissue and cells even during the pre-clinical phase of a disease. As Raman-based microspectroscopy allows for non-invasive, contact-free and label-free investigation of living tissue not disturbed by the presence of water, it contains high potential for biomedical diagnostics. Our experimental approach towards CARS-based in vitro tissue characterization is discussed. The focus lies on the joint use of linear Raman microspectroscopy and CARS microscopy. While linear Raman microspectroscopy is used to obtain the information on critical Raman marker bands at selected spatial positions within the sample, CARS microscopy is focussing on fast image generation using the previously defined Raman marker bands. Complementarily, secondharmonic generation imaging was applied to the samples. Furthermore, we present innovative concepts for CARS-image analysis.

[1]  Andreas Zumbusch,et al.  Coherent anti-Stokes Raman scattering microscopy , 1999 .

[2]  Hung-Che Chen,et al.  Quantitative assessment of hepatic fat of intact liver tissues with coherent anti-stokes Raman scattering microscopy. , 2009, Analytical chemistry.

[3]  T. Gustavsson,et al.  Image analysis in nonlinear microscopy. , 2008, Journal of the Optical Society of America. A, Optics, image science, and vision.

[4]  Maxwell Zimmerley,et al.  Quantitative detection of chemical compounds in human hair with coherent anti-Stokes Raman scattering microscopy. , 2009, Journal of biomedical optics.

[5]  Jürgen Popp,et al.  Different contrast information obtained from CARS and nonresonant FWM images , 2009 .

[6]  K Svanberg,et al.  Fluorescence diagnostics and kinetic studies in the head and neck region utilizing low-dose delta-aminolevulinic acid sensitization. , 1998, Cancer letters.

[7]  H Rigneault,et al.  Coherent anti-Stokes Raman scattering (CARS) microscopy imaging at interfaces: evidence of interference effects. , 2007, Optics express.

[8]  B. Dietzek,et al.  Raman and CARS microspectroscopy of cells and tissues. , 2009, The Analyst.

[9]  Jürgen Popp,et al.  Analysis of the cytochrome distribution via linear and nonlinear Raman spectroscopy. , 2010, The Analyst.

[10]  Jürgen Popp,et al.  A comparative Raman and CARS imaging study of colon tissue , 2009, Journal of biophotonics.

[11]  Riccardo Cicchi,et al.  Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy , 2009, Journal of biophotonics.

[12]  E. Petricoin,et al.  The Vision for a New Diagnostic Paradigm , 2003 .

[13]  W. Marsden.  I and J , 2012 .

[14]  Jürgen Popp,et al.  Raman spectroscopy--a prospective tool in the life sciences. , 2003, Chemphyschem : a European journal of chemical physics and physical chemistry.

[15]  Jürgen Popp,et al.  Quantitative CARS microscopic detection of analytes and their isotopomers in a two-channel microfluidic chip. , 2009, Small.

[16]  Roberto Pini,et al.  Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging. , 2009, Optics express.

[17]  Jürgen Popp,et al.  Separation of CARS image contributions with a Gaussian mixture model. , 2010, Journal of the Optical Society of America. A, Optics, image science, and vision.

[18]  R. Stephenson.  A and V , 1962, The British journal of ophthalmology.

[19]  Jürgen Popp,et al.  Raman spectroscopy at the beginning of the twenty-first century , 2006 .

[20]  Annika Enejder,et al.  Coherent Anti-Stokes Raman Scattering Microscopy , 2012 .

[21]  Christoph Krafft,et al.  Raman and FTIR microscopic imaging of colon tissue: a comparative study , 2008, Journal of biophotonics.

[22]  S. Lockett,et al.  Coherent anti‐stokes Raman scattering microscopy: A biological review , 2006, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[23]  Jürgen Popp,et al.  Tunable narrow band filter for CARS microscopy , 2010 .

[24]  Kazuyoshi Itoh,et al.  Three-Dimensional Unstained Live-Cell Imaging Using Stimulated Parametric Emission Microscopy , 2009 .

[25]  Markus J. Buehler,et al.  Nature designs tough collagen: Explaining the nanostructure of collagen fibrils , 2006, Proceedings of the National Academy of Sciences.

[26]  Jürgen Popp,et al.  Tunable light source for narrowband laser excitation: application to Raman spectroscopy , 2009 .

[27]  Annika Enejder,et al.  Image analysis in non-linear microscopy , 2008 .

[28]  Jürgen Popp,et al.  Three-dimensional molecular mapping of a multiple emulsion by means of CARS microscopy. , 2008, The journal of physical chemistry. B.