Metal-Affinity Separations: A New Dimension in Protein Processing

Rapid growth in the preparative and high-resolution analytical applications of metal-affinity chromatography demonstrate the appeal of metal recognition as a basis for protein separations. Stable, inexpensive chelated metals effectively mimic bio-specific interactions, providing selective ligands for protein binding. This article reviews recent progress in understanding the mechanisms of metal-protein recognition that underlie metal-affinity separations. Also discussed are schemes for integrating metal-affinity purifications into the expression and bioprocessing of re-combinant proteins. Promising future developments include new metal-affinity processes for analytical and preparative-scale separations and a range of techniques for enhancing the selectivity of metal-affinity separations.

[1]  J. Porath.  IMAC—Immobilized metal ion affinity based chromatography , 1988 .

[2]  C. Pidgeon,et al.  Immobilized iminodiacetic acid metal peptide complexes. Identification of chelating peptide purification handles for recombinant proteins , 1987 .

[3]  E. Hochuli,et al.  New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. , 1987, Journal of chromatography.

[4]  J. Porath,et al.  Surface topography of histidine residues: a facile probe by immobilized metal ion affinity chromatography. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[5]  L. Post,et al.  Active Human Tissue Plasminogen Activator Secreted from Insect Cells Using a Baculovirus Vector , 1988, Biotechnology and applied biochemistry.

[6]  V. Davankov,et al.  Ligand exchange chromatography , 1988 .

[7]  J. Porath,et al.  Purification of Two Muscle Enzymes by Chromatography on Immobilized Ferric Ions , 1989, Biotechnology and applied biochemistry.

[8]  C. Horváth,et al.  Metal chelate-interaction chromatography of proteins with iminodiacetic acid-bonded stationary phases on silica support. , 1986, Journal of chromatography.

[9]  F. S. Stover,et al.  Capillary zone electrophoresis of histidine-containing compounds. , 1989, Journal of chromatography.

[10]  S. L. Le Grice,et al.  Rapid purification of homodimer and heterodimer HIV-1 reverse transcriptase by metal chelate affinity chromatography. , 1990, European journal of biochemistry.

[11]  J. Porath.  Metal Ion — Hydrophobic, Thiophilic and II‐Electron Governed Interactions and their Application to Salt‐Promoted Protein Adsorption Chromatography , 1987 .

[12]  E. Sulkowski.  Purification of proteins by IMAC , 1985 .

[13]  F. Arnold,et al.  Metal affinity extraction of human hemoglobin in an aqueous polyethylene glycol-sodium sulfate two-phase system , 1990 .

[14]  Mookambeswaran Vijayalakshmi,et al.  Cell surface interactions with metal chelates. , 1989, Journal of Chromatography A.

[15]  M. Vijayalakshmi.  Pseudobiospecific ligand affinity chromatography , 1989 .

[16]  M. Klapper,et al.  The independent distribution of amino acid near neighbor pairs into polypeptides. , 1977, Biochemical and biophysical research communications.

[17]  A. Plückthun,et al.  The Functional Expression of Antibody Fv Fragments in Ischhuchia coli: Improved Vectors and a Generally Applicable Purification Technique , 1991, Bio/Technology.

[18]  G. Wuenschell,et al.  Metal affinity precipitation of proteins , 1989, Biotechnology and applied biochemistry.

[19]  C. Pidgeon,et al.  Chelating peptide-immobilized metal ion affinity chromatography. A new concept in affinity chromatography for recombinant proteins. , 1988, The Journal of biological chemistry.

[20]  H. Hsiung,et al.  Chelating peptide-immobilized metal ion affinity chromatography , 1989 .

[21]  J. Porath,et al.  Metal chelate affinity chromatography, a new approach to protein fractionation , 1975, Nature.

[22]  B. Haymore.  Introducing strong metal-binding sites onto surfaces of proteins for facile and efficient metal-affinity purifications , 1992 .

[23]  G. Grandi,et al.  Immobilized metal-ion affinity chromatography of human growth hormone. , 1989, Journal of chromatography.

[24]  J. Porath,et al.  Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials. Serum protein affinities for gel-immobilized iron and nickel ions. , 1983, Biochemistry.

[25]  M. Uhlén,et al.  Gene fusions for purpose of expression: an introduction. , 1990, Methods in enzymology.

[26]  F. Arnold,et al.  Characterization of His-X3-His sites in α-helices of synthetic metal-binding bovine somatotropin , 1991 .

[27]  F. Arnold,et al.  Cu(II)‐Binding properties of a cytochrome c with a synthetic metal‐binding site: His‐X3‐His in an α‐helix , 1991, Proteins.

[28]  T. Yip,et al.  Multiple DNA-binding estrogen receptor forms resolved by interaction with immobilized metal ions. Identification of a metal-binding domain. , 1989, The Journal of biological chemistry.

[29]  F. Arnold,et al.  A mathematical model for metal affinity protein partitioning , 1990, Biotechnology and bioengineering.

[30]  C. Horváth,et al.  Combined lectin-affinity and metal-interaction chromatography for the separation of glycophorins by high-performance liquid chromatography. , 1988, Journal of chromatography.

[31]  R. Gentz,et al.  Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent , 1988, Bio/Technology.

[32]  E. Sulkowski.  The saga of IMAC and MIT. , 1989, BioEssays : news and reviews in molecular, cellular and developmental biology.

[33]  B. Karger,et al.  High-performance immobilized-metal affinity chromatography of proteins on iminodiacetic acid silica-based bonded phases. , 1986, Journal of chromatography.

[34]  T. Yip,et al.  Evaluation of the interaction of peptides with Cu(II), Ni(II), and Zn(II) by high-performance immobilized metal ion affinity chromatography. , 1989, Analytical biochemistry.

[35]  M. Uhlén,et al.  Immobilization and affinity purification of recombinant proteins using histidine peptide fusions. , 1989, European journal of biochemistry.

[36]  Richard J. Sundberg,et al.  Interactions of histidine and other imidazole derivatives with transition metal ions in chemical and biological systems , 1974 .

[37]  F. Arnold,et al.  Aqueous two-phase metal affinity extraction of heme proteins , 1990 .

[38]  F. Arnold,et al.  Chiral copper-chelate complexes alter selectivities in metal affinity protein partitioning. , 1991, Journal of chromatography.