7-methylpterin in methanogenic bacteria

A blue fluorescent compound was extracted and purified from cells of Methanobacterium thermoautotrophicum. The compound was identified as 7-methylpterin on the basis of its (physico-) chemical properties and by comparison with 7-methylpterin prepared by organic synthesis. The compound is present in all methanogenic bacteria studied so far and it provides methanogenic bacteria the characteristic blue fluorescence observed upon fluorescence microscopy.