The cDNA sequencing project.

The Dictyostelium discoideum cDNA sequencing project started in 1995, preceding the genome sequencing project. Altogether, 14 cDNA libraries, including full-length ones, were constructed from five different stages of growth and asexual and sexual development, from which nearly 100,000 randomly chosen clones were sequenced to yield over 150,000 expressed sequence tags (ESTs). The data have been publicized online to facilitate clone distribution and collaboration using the whole clone set for microarray analyses. The EST reads were assembled to 6700 independent genes, which constitute about 55% of the total estimated Dictyostelium genes. Utilization of wet and dry resources have contributed to the understanding of the genetic system controlling the multicellular development in Dictyostelium.

[1]  K. Maruyama,et al.  Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. , 1994, Gene.

[2]  Ezgi O. Booth,et al.  Epistasis analysis with global transcriptional phenotypes , 2005, Nature Genetics.

[3]  W. Loomis,et al.  Identification of Genes Dependent on the MADS Box Transcription Factor SrfA in Dictyostelium discoideum Development , 2004, Eukaryotic Cell.

[4]  M. Katoh,et al.  Spatial expression patterns of genes involved in cyclic AMP responses in Dictyostelium discoideum development , 2001, Development, growth & differentiation.

[5]  Y. Kohara,et al.  Construction of a gamete-enriched gene pool and RNAi-mediated functional analysis in Dictyostelium discoideum , 2003, Mechanisms of Development.

[6]  Aaron P. Campbell,et al.  Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[7]  A Suyama,et al.  Diverse transcriptional initiation revealed by fine, large‐scale mapping of mRNA start sites , 2001, EMBO reports.

[8]  T Hwa,et al.  Expression patterns of cell-type-specific genes in Dictyostelium. , 2001, Molecular biology of the cell.

[9]  M. Katoh,et al.  Developmental changes in the spatial expression of genes involved in myosin function in Dictyostelium. , 2000, Developmental biology.

[10]  R. Kay,et al.  The role of DIF-1 signaling in Dictyostelium development. , 2000, Molecular cell.

[11]  Hideko Urushihara,et al.  A transcriptional profile of multicellular development in Dictyostelium discoideum. , 2002, Development.

[12]  Wei Zhu,et al.  Optimal spliced alignment of homologous cDNA to a genomic DNA template , 2000, Bioinform..

[13]  M. Katoh,et al.  A STAT-regulated, stress-induced signalling pathway in Dictyostelium , 2003, Journal of Cell Science.

[14]  W. Loomis,et al.  Control of Cell Type Proportioning in Dictyostelium discoideum by Differentiation-Inducing Factor as Determined by In Situ Hybridization , 2004, Eukaryotic Cell.

[15]  T. Saito,et al.  The Dictyostelium developmental cDNA project: generation and analysis of expressed sequence tags from the first-finger stage of development. , 1998, DNA research : an international journal for rapid publication of reports on genes and genomes.

[16]  F. Rivero.  mRNA processing in Dictyostelium: sequence requirements for termination and splicing. , 2002, Protist.

[17]  M. Zvelebil,et al.  Dd-STATb, a Dictyostelium STAT protein with a highly aberrant SH2 domain, functions as a regulator of gene expression during growth and early development , 2003, Development.

[18]  David L. Steffen,et al.  The genome of the social amoeba Dictyostelium discoideum , 2005, Nature.

[19]  Hiderou Yoshida,et al.  dutA RNA functions as an untranslatable RNA in the development of Dictyostelium discoideum , 1994, Nucleic Acids Res..

[20]  L. Eichinger,et al.  Dictyostelium transcriptional host cell response upon infection with Legionella , 2006, Cellular microbiology.

[21]  C. Shaw,et al.  An orderly retreat: Dedifferentiation is a regulated process. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[22]  P. Green,et al.  Consed: a graphical tool for sequence finishing. , 1998, Genome research.

[23]  Y. Kohara,et al.  Analyses of cDNAs from growth and slug stages of Dictyostelium discoideum. , 2004, Nucleic acids research.

[24]  J. Bonner,et al.  Evidence for the formation of cell aggregates by chemotaxis in the development of the slime mold Dictyostelium discoideum. , 1947, The Journal of experimental zoology.